Extracellular vesicles in disease

Distinct melanoma EV subpopulations reflect immune- and stress-induced tumor states

This study identifies immune- and stress-induced changes in melanoma EV subpopulations and demonstrates their detection in human plasma.

Aldehyde dehydrogenase 1A3 detection in extracellular vesicles from breast cancer cell lines by nano-flow cytometry

This study detects ALDH1A3 enzymatic activity in exosomes from breast cancer cell lines using nano-flow cytometry, providing functional evidence of its presence in cancer-derived exosomes.

Impact of Isolation and Storage Methods on the Properties of Neural Extracellular Vesicles

This study evaluates how different isolation and storage methods affect neural extracellular vesicles' properties and immunomodulatory activity.

Super-Resolved Single Small Extracellular Vesicle Assay enabled by a Plasmonic Nanohole Array

This paper introduces a nanoplasmonic platform for high-resolution single small extracellular vesicle quantification and subpopulation differentiation, achieving a detection limit of 143 sEVs/μL.

Analysis of Plasma Extracellular Vesicles in Normal-Weight and Overweight Type 2 Diabetes Using Multimodal SERS and RNA-Seq

The paper uses multimodal SERS and RNA-Seq to analyze plasma extracellular vesicles in T2DM patients, identifying subtype-specific molecular features and miRNA expression patterns.

Plasma-derived extracellular vesicles as potential biomarkers and mediators of functional alterations in MELAS

This study investigates plasma-derived extracellular vesicles as biomarkers for MELAS and their functional impact on mitochondrial respiration in skeletal muscle myotubes.

Lyophilization Maintains the Storage Stability and Bioactivity of Mesenchymal Stem Cell–Derived Extracellular Vesicles

This study shows lyophilization preserves the structural and functional properties of mesenchymal stem cell-derived extracellular vesicles across various storage temperatures.

Lyophilized Extracellular Vesicles Retain Regenerative Activity and Accelerate Wound Healing

This study shows lyophilized extracellular vesicles stored at -80°C retain structural integrity and therapeutic efficacy in wound healing.

HMEC-1 extracellular vesicles as regulators of endothelial cell activation under inflammation

This study isolates and characterizes HMEC-1 extracellular vesicles, showing they enhance wound closure and have dual inflammatory effects on endothelial cells.

Glioblastoma-derived extracellular vesicles released after radiation promote cognitive impairment through NFκB-mediated microglial activation

This study shows that radiation-induced glioblastoma-derived extracellular vesicles impair cognition by activating microglia via NFκB and causing neurotoxic H2O2 release.

Comparison of extracellular vesicles isolation methods reveals method-dependent protein and miRNA profiles in saliva

This study compares EV isolation methods in saliva, showing method-dependent differences in protein and miRNA profiles, with immunoaffinity capture isolating distinct EV populations.

Stable Vesicle-Associated BDNF from Embryonic and Young Cortical Extracellular Vesicles

This study shows that BDNF in extracellular vesicles from embryonic and young cortex maintains neuroprotective signaling, while aged vesicles lack BDNF and fail to trigger protection.

Red Blood Cell-derived Extracellular Vesicles enable Cisplatin and Cetuximab combined Therapy against Triple-Negative Breast Cancer

This paper develops a nanoplatform using red blood cell-derived extracellular vesicles to deliver Cetuximab and cisplatin, enhancing cancer cell killing and reducing toxicity in triple-negative breast cancer.

Recipient cell identity governs intracellular transport and membrane interactions of extracellular vesicles across species

This study investigates how recipient cell identity influences intracellular transport and membrane interactions of extracellular vesicles across species, finding that recipient-cell environment has a greater impact than EV origin.

Tumor extracellular vesicle RNA profiling predicts treatment response in pediatric diffuse midline glioma

This paper shows that analyzing RNA in tumor-derived extracellular vesicles from blood can predict treatment response in pediatric diffuse midline glioma.

Monocytes shape the neuroprotective and immunomodulatory effects of mesenchymal stromal cell-derived extracellular vesicles

This study shows that monocytes are essential for the neuroprotective effects of MSC-EVs in stroke, as their depletion negates these benefits and leads to immune system dysregulation.

Extracellular Vesicle Biomarkers in the Aqueous Humor Correlate with Retinoblastoma Seeding

The paper correlates CD133 levels in retinoblastoma aqueous humor extracellular vesicles with disease severity and seeding patterns.

Engineering Carbon Nanotube Quantum Well Defects with Recognition Tripeptides for Optical Detection of Extracellular Vesicles in Plasma

This paper develops a carbon nanotube sensor with RGD tripeptides to detect extracellular vesicles in plasma with high sensitivity.

Hollow-fibre biomanufacturing and cell-free engineering of HEK293 extracellular vesicles

This paper demonstrates cell-free engineering of HEK293 extracellular vesicles with membrane fusion proteins and evaluates methods for assessing engineering efficiency.

Therapeutic poxviruses induce the secretion of immunostimulating and anti-tumoral extracellular vesicles

This study shows that therapeutic poxviruses induce the secretion of immunostimulating and anti-tumoral extracellular vesicles, which can reduce tumor growth in mice.

Secreted phospholipase PLA2G12A-driven lysophospholipid signaling via lipolytic modification of extracellular vesicles facilitates pathogenic Th17 differentiation

This study identifies PLA2G12A's role in Th17 differentiation through lysophospholipid signaling via extracellular vesicles, showing therapeutic potential for Th17-related diseases.

Extracellular vesicles from Manila clam (Ruditapes philippinarum): tailored isolation from hemolymph and insights into water-derived vesicles

This study isolates extracellular vesicles from Manila clam hemolymph and conditioned water, revealing distinct metabolic signatures and demonstrating their potential as biomarkers.

Electrokinetic active grooves for liposome capture, confinement, trajectory analysis and controlled release

This paper introduces RECON grooves, an electrokinetic platform for controlled capture, retention, and release of nanoscale vesicles with optical trajectory analysis.

Characterization of Rosa damascena Callus-derived Exo-some-like Vesicles and Their Multifunctional Activities in Skin-related Cellular Models

This study characterizes Rosa damascena callus-derived exosome-like vesicles and shows they enhance collagen synthesis, reduce melanin production, and suppress inflammation in skin-related cellular models.

CD133 shapes extracellular vesicle cargo and angiogenic function in basal-like triple-negative breast cancer

This study identifies CD133 as a regulator of EV cargo and angiogenic function in BL-TNBC, showing its role in shaping EV composition and promoting tumor angiogenesis.

Benchmarking five extracellular vesicle proteomics workflows, including low-input Exo-insert and Exo-SP3, for deep mass spectrometry profiling

The paper benchmarks five proteomics workflows for sEV analysis, showing Exo-insert and Exo-SP3 can identify ~1100 proteins from low-input samples, with performance varying by sEV source.

Isolation and Characterization of Extracellular Vesicles from Mouse Retina Tissue

This paper provides a standardized protocol for isolating and characterizing extracellular vesicles from mouse retinal tissue, improving the study of in vivo EV biology.

The molecular architecture of tunneling nanotubes

This study characterizes the molecular architecture of tunneling nanotubes (TNTs) using live-cell imaging and cryo-ET, revealing a complex structure with actin, microtubules, and organelles.

Intranasal Delivery of Hypoxia Primed Whartons Jelly MSC Derived sEVs Reprograms Neuroimmune Signalling and Ameliorates Behavioural Deficits in a Valproic Acid Induced Mouse Model of Autism Spectrum Disorder

This study shows that hypoxia-primed Wharton's jelly MSC-derived sEVs improve behavioral and neuroinflammatory outcomes in a mouse model of ASD through enhanced neuroprotective effects.

Plasma Extracellular Vesicle and miRNA Profiles in Pregnancies Complicated by Fetal Congenital Heart Defects

This study examines extracellular vesicles and miRNA profiles in maternal and umbilical cord plasma from pregnancies with fetal congenital heart defects compared to controls, finding altered EV counts and miRNA levels.