Trypanosoma species research and implications

Roles and interactions of the specialized initiation factors EIF4E2, EIF4E5 and EIF4E6 in Trypanosoma brucei: EIF4E2 maintains the abundances of S-phase mRNAs

The paper investigates the roles of EIF4E2, EIF4E5, and EIF4E6 in Trypanosoma brucei, finding that EIF4E2 maintains S-phase mRNA abundance and is involved in mRNA stabilization.

Core-shell DNA-cholesterol nanoparticles exert lysosomolytic activity in African trypanosomes

The paper describes DNA-cholesterol nanoparticles that kill Trypanosoma brucei by disrupting lysosomal membrane integrity and demonstrate a dual-target approach using DNAzymes.

Genomic occupancy of the bromodomain protein Bdf3 is dynamic during differentiation of African trypanosomes from bloodstream to procyclic forms

The paper investigates dynamic changes in Bdf3 chromatin occupancy during Trypanosoma brucei differentiation from bloodstream to insect stage, finding increased occupancy at genes involved in surface protein remodeling.

The genomic basis of host and vector specificity in non-pathogenic trypanosomatids

This paper sequences and compares the genomes of two non-pathogenic trypanosomes to identify genomic features associated with host and vector specificity.

Several different sequences are implicated in bloodstream-form-specific gene expression in Trypanosoma brucei

The paper identifies multiple regulatory sequences in the 3'-untranslated regions of mRNAs that control bloodstream-form-specific gene expression in Trypanosoma brucei.

Single-cell transcriptomics reveals expression profiles of Trypanosoma brucei sexual stages

The paper uses single-cell RNA sequencing to identify and characterize sexual stages in Trypanosoma brucei, revealing gene expression patterns associated with gamete formation.

Oxidative phosphorylation is required for powering motility and development of the sleeping sickness parasite Trypanosoma brucei within the tsetse fly vector

This study shows that oxidative phosphorylation is essential for Trypanosoma brucei migration within tsetse flies but not for initial colonization.

Intramolecular interactions enhance the potency of gallinamide A analogs against Trypanosoma cruzi

The paper evaluates gallinamide A and its analogs against Trypanosoma cruzi, finding that intramolecular π-π stacking interactions enhance potency by improving solubility and permeability.

The Trypanosoma cruzi kinetoplast DNA minicircle sequences transfer biomarker of the multidrug treatment of Chagas disease

The paper demonstrates that T. cruzi kinetoplast DNA minicircle sequences integrate into host genomes, and shows that multidrug treatment reduces their integration rate in mice.

A novel high-content phenotypic screen to identify inhibitors of mitochondrial DNA maintenance in trypanosomes

The paper develops a high-content screen to identify inhibitors of mitochondrial DNA maintenance in trypanosomes and identifies a novel kDNA-targeting compound.

The spliced leader RNA silencing (SLS) pathway in Trypanosoma brucei is induced by perturbations of endoplasmic reticulum, Golgi, or mitochondrial proteins factors and functional analysis of SLS inducing kinase, PK3

The paper shows that the SLS pathway in Trypanosoma brucei is triggered by disruptions in ER, Golgi, or mitochondrial protein handling and identifies PK3 phosphorylation as critical for its activation.

Mechanisms of translation repression by the EIF4E1-4EIP cap-binding complex of Trypanosoma brucei: potential roles of the NOT complex and a terminal uridylyl transferase

This study investigates the role of the EIF4E1-4EIP cap-binding complex in translation repression in Trypanosoma brucei, showing that 4EIP stabilizes EIF4E1 and recruits TUT3, but NOT complex may be involved in 4EIP function.

DNA double strand break position leads to distinct gene expression changes and regulates VSG switching pathway choice.

The paper investigates how the position of DNA double strand breaks influences gene expression and VSG switching pathway choice in Trypanosoma brucei.

A DOT1B/Ribonuclease H2 protein complex is involved in R-loop processing, genomic integrity and antigenic variation in Trypanosoma brucei

Site-specific epigenetic marks in Trypanosoma brucei transcription termination, antigenic variation, and proliferation

This study identifies the roles of histone variants H3v and H4v and base J in transcription termination, antigenic variation, and proliferation in Trypanosoma brucei.

The establishment of variant surface glycoprotein monoallelic expression revealed by single-cell RNA-seq of Trypanosoma brucei in the tsetse fly salivary glands.

This study uses scRNA-seq to analyze Trypanosoma brucei development in tsetse fly salivary glands, revealing dynamic VSG gene activation and monoallelic expression establishment.

The RNA-binding protein DRBD18 regulates processing and export of the mRNA encoding Trypanosoma brucei RNA-binding protein 10

The paper shows DRBD18 regulates RBP10 mRNA processing and export in Trypanosoma brucei by interacting with its 3-UTR, affecting mRNA stability and localization.

In vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.

The paper describes two new Trypanosoma brucei brucei strains with minimal in vitro passage and reports gene copy-number changes associated with culture adaptation.

Maxicircle architecture and evolutionary insights into Trypanosoma cruzi complex

The paper sequences maxicircles from T. cruzi strains using long-read sequencing, revealing their architecture, evolutionary relationships, and proposing a new nomenclature.

Monomorphic Trypanozoon: towards reconciling phylogeny and pathologies

The paper re-analyzes genomic data to define clades of monomorphic Trypanozoon species and identifies lineage-specific variation, challenging traditional classifications and suggesting evolutionary relationships.

The genomic basis of domestic colonisation and dispersal in Chagas disease vectors

This study investigates the genomic basis of domestic colonization in Chagas disease vectors, finding that landscape elevation drives population structure and identifying genetic loci associated with adaptation to domestic environments.

Repeat-driven generation of antigenic diversity in a major human pathogen, Trypanosoma cruzi

The paper presents an improved genome assembly of Trypanosoma cruzi and reveals a unique genomic structure involving subtelomeric regions and retrotransposons contributing to antigenic variation.

Chromatin-associated protein complexes link DNA base J and transcription termination in Leishmania

The paper identifies JBP3, a J-binding protein in Leishmania, which associates with complexes involved in transcription termination and chromatin remodeling, enabling polycistronic transcription and post-transcriptional regulation.

Antigenic variation by switching inter-chromosomal interactions with an RNA splicing locus in trypanosomes

The paper identifies a novel mechanism in trypanosomes where inter-chromosomal interactions between an antigen coding gene and an mRNA splicing locus ensure monogenic antigen expression.

Tsetse blood-meal sources, endosymbionts, and trypanosome infections provide insight into African trypanosomiasis transmission in the Maasai Mara National Reserve, a wildlife-human-livestock interface

The paper identifies tsetse fly blood-meal sources, endosymbiont infections, and trypanosome infections in the Maasai Mara National Reserve, finding that hippopotamus and buffalo are significant hosts and that Sodalis glossinidius endosymbionts correlate with higher trypanosome infection rates.

Unusual Features of the Membrane Kinome of Trypanosoma brucei

The paper identifies and characterizes the membrane-associated kinome of Trypanosoma brucei, revealing unique structural features and subcellular localization compared to other eukaryotes.

Identification of Trypanosoma brucei gambiense and T. b. rhodesiense in vectors using multiplexed high-resolution melt analysis.

The paper develops a high-resolution melt assay to identify Trypanosoma brucei gambiense and T. b. rhodesiense in tsetse flies, offering a rapid and specific alternative to multiple PCR methods.

A Trypanosoma cruzi Zinc Finger protein that controls expression of epimastigote specific genes and affects metacyclogenesis

The paper identifies a T. cruzi zinc finger protein, TcZC3H12, that regulates epimastigote gene expression and negatively affects metacyclogenesis.

What happens after a blood meal? A transcriptome response from the main tissues involved in egg production in Rhodnius prolixus, an insect vector of Chagas disease

The paper examines how a blood meal affects mRNA expression in the CNS, fat body, and ovaries of Rhodnius prolixus to promote egg production, identifying tissue-specific responses related to metabolism, signaling, and immunity.

The trypanosome Variant Surface Glycoprotein mRNA is stabilized by an essential unconventional RNA-binding protein

This study identifies CFB2 as an essential RNA-binding protein that stabilizes VSG mRNA in trypanosomes, crucial for antigenic variation and cell survival.