G. P. Nolan

Epstein-Barr Virus Orchestrates Spatial Reorganization and Immunomodulation within the Classic Hodgkin Lymphoma Tumor Microenvironment

This study uses spatial multi-omics to compare EBV-positive and EBV-negative cHL, identifying distinct TME features and immunological signatures linked to EBV.

A Spatial Multi-Modal Dissection of Host-Microbiome Interactions within the Colitis Tissue Microenvironment

This study introduces MicroCart, a framework for spatial multi-omics analysis of host-microbiome interactions in colitis, revealing tissue-level immune responses and microbial shifts.

Multimodal and spatially resolved profiling identifies distinct patterns of T-cell infiltration in nodal B-cell lymphoma entities

A single-cell multi-omic and spatial atlas of B cell lymphomas reveals differentiation drives intratumor heterogeneity

This study reveals that cellular differentiation drives intratumor heterogeneity in B-cell lymphomas, with distinct maturation states associated with different survival outcomes.

Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

MAPS: Pathologist-level cell type annotation from tissue images through machine learning

MAPS provides pathologist-level cell type annotation from spatial proteomics data using machine learning, outperforming existing methods in speed and accuracy.

Concerted epithelial and stromal changes during progression of Barrett's Esophagus to invasive adenocarcinoma exposed by multi-scale, multi-omics analysis

This study uses multi-omics analysis to show coordinated changes in epithelial, stromal, and immune compartments during Barrett's Esophagus progression to adenocarcinoma, revealing early appearance of cancer-associated fibroblasts and immunosuppressive microenvironment.

A spatial map of human macrophage niches links tissue location with function

The paper identifies distinct macrophage niches in human breast and colon tissues using single-cell RNA sequencing and imaging, linking their spatial distribution to functional roles.

A single cell spatial temporal atlas of skeletal muscle reveals cellular neighborhoods that orchestrate regeneration and become disrupted in aging

The paper constructs a single cell spatial temporal atlas of skeletal muscle to reveal cellular neighborhoods involved in regeneration and their disruption in aging.

Aldehyde dehydrogenase 3A1 deficiency leads to mitochondrial dysfunction and impacts salivary gland stem cell self-renewal, differentiation and survival

This study shows that ALDH3A1 deficiency causes mitochondrial dysfunction and impairs salivary gland stem cell self-renewal, differentiation, and survival.

SARS-CoV-2 infects human adipose tissue and elicits an inflammatory response consistent with severe COVID-19

This study shows SARS-CoV-2 infects human adipose tissue, causing inflammation linked to severe COVID-19 outcomes in obese individuals.

Spatial epitope barcoding reveals subclonaltumor patch behaviors

The paper develops a method to track subclonal tumor patch behaviors using spatial epitope barcoding and imaging, revealing emergent properties in cancer cell states.

Virus-Dependent Immune Conditioning of Tissue Microenvironments

The paper develops PANINI-MIBI to analyze DNA, RNA, and protein in SIV-infected tissues, revealing IL10 upregulation and macrophage polarization linked to viral latency.

Integrated plasma proteomic and single-cell immune signaling network signatures demarcate mild, moderate, and severe COVID-19

This study integrates plasma proteomics and single-cell immune features to classify COVID-19 severity and identify biological signatures of disease progression.

Single-cell profiling of Ebola virus infection in vivo reveals viral and host transcriptional dynamics

This study uses single-cell transcriptomics and protein profiling to analyze EBOV infection in rhesus monkeys, revealing changes in immune cell dynamics and viral/host gene expression.

Activation of JUN in fibroblasts promotes pro-fibrotic programme and modulates protective immunity

This study shows JUN activation in fibroblasts drives fibrosis and modulates immunity, with therapeutic potential through targeting IL-6, CD47, and PD-L1.

Mitogen-independent cell cycle progression in B lymphocytes

This study shows B lymphocytes can undergo multiple mitosis-independent divisions after the first mitosis, driven by unique G1 phase characteristics and limited by apoptosis, with Birc5 playing an essential role.

Trajectories from Snapshots: Integrated proteomic and metabolic single-cell assays reveal multiple independent adaptive responses to drug tolerance in a BRAF-mutant melanoma cell line

The paper uses integrated proteomic and metabolic single-cell assays to identify two distinct adaptive pathways in BRAF-mutant melanoma cells during drug treatment.

Coordinated cellular neighborhoods orchestrate antitumoral immunity at the colorectal cancer invasive front

The paper identifies cellular neighborhoods in colorectal cancer that correlate with antitumoral immunity and patient survival.

A Systems Immunology Approach Identifies Cytokine-Induced STAT Signaling Pathways Critical to Rheumatoid Arthritis Disease Activity and Treatment Response

Profiling Myelodysplastic Syndromes by Mass Cytometry Demonstrates Abnormal Progenitor Cell Phenotype and Differentiation

This paper uses mass cytometry to analyze MDS patient samples, identifying abnormal surface markers and differentiation patterns not detectable with traditional methods.

Phospho-tuning immunity through Denisovan, modern human, and mouse TNFAIP3 gene variants

The paper identifies TNFAIP3 variants in humans, Denisovans, and mice that modulate A20 phosphorylation, revealing a genetic mechanism for balancing immunity and microbial tolerance.

Cell type-specific monoclonal antibody cross-reactivity screening in non-human primates and development of comparative immunophenotyping panels for CyTOF

The paper screens monoclonal antibodies for cross-reactivity in non-human primates and develops immunophenotyping panels for CyTOF.

A comprehensive atlas of immunological differences between humans, mice and non-human primates

The paper compares immunological differences between humans, mice, and non-human primates using CyTOF mass cytometry, identifying species-specific immune responses and correlations with viral serostatus.

Variation of immune cell responses in humans reveals sex-specific coordinated signaling across cell types

The paper profiles immune cell responses in humans to identify sex-specific signaling patterns and provides reference ranges for immune markers.