S. Chen

Perturbomics of tumor-infiltrating NK cells

This study identifies genetic factors limiting NK cell function in tumors using CRISPR screens and single-cell sequencing, highlighting CALHM2 as a target for improving NK cell-based therapies.

CTLA-4 tail fusion enhances CAR-T anti-tumor immunity

This study enhances CAR-T cell efficacy by fusing CTLA-4 cytoplasmic tails to CARs, improving anti-tumor responses in a leukemia model.

Rational design of immune gene therapy combinations via in vivo CRISPR activation screen of tumor microenvironment modulators

This study uses in vivo CRISPR activation screens to identify immunostimulatory factors that enhance anti-tumor immunity and develop effective gene therapy combinations.

Therapeutic immune cell engineering with an mRNA : AAV-Sleeping Beauty composite system

The paper introduces a composite gene delivery system called MAJESTIC for efficient and stable engineering of therapeutic immune cells, showing improved cell viability and transgene expression compared to existing methods.

Immunogenetic metabolomics revealed key enzymes that modulate CAR-T metabolism and function

The paper identifies ADA as a metabolic regulator that enhances CAR-T cell function and tumor clearance through improved metabolism and reduced exhaustion.

Analyzing genomic alterations involved in fluoroquinolone-resistant development in Staphylococcus aureus

This study investigates genomic and gene expression changes in Staphylococcus aureus after fluoroquinolone exposure, identifying mutations and overexpressed genes linked to multidrug resistance.

NS5A domain I antagonises PKR to facilitate the assembly of infectious hepatitis C virus particles

The paper identifies NS5A domain I mutations that block HCV virus assembly and shows this effect is mediated through interaction with PKR, an antiviral pathway.

Optical tools for visualizing and controlling human GLP-1 receptor activation with high spatiotemporal resolution

This paper introduces GLPLight1, a genetically-encoded sensor for high spatiotemporal resolution visualization of GLP1R activation, and demonstrates its use in optical control of receptor activation.

Image-guided deployment and monitoring of a novel tungsten nanoparticle-infused radiopaque absorbable inferior vena cava filter in pig

This study evaluates tungsten nanoparticle-infused radiopaque absorbable inferior vena cava filters in pigs, showing improved radiopacity but reduced in vivo visibility over time.

ABI5-FLZ13 Module Transcriptionally Represses Growth-related Genes to Delay Seed Germination in Response to ABA

This study identifies FLZ13 as an ABI5-interacting protein that co-represses growth-related genes to delay seed germination in response to ABA.

Identification and characterization of a proliferative cell population in estrogen receptor-positive metastatic breast cancer through spatial and single-cell transcriptomics

The paper identifies a proliferative cell population in ER+ metastatic breast cancer using spatial and single-cell transcriptomics, showing its role in estrogen-dependent growth and clinical outcomes.

Redefining hematopoietic progenitor cells and reforming the hierarchy of hematopoiesis

This study redefines hematopoietic progenitor cells and reformulates the hematopoiesis hierarchy using a transcriptional atlas, identifying key transcription factors and distinct lineage commitment processes.

Phenotypically Complex Living Materials Containing Engineered Cyanobacteria

This paper develops a 3D-printed biocomposite material containing engineered cyanobacteria that respond to chemical stimuli by producing functional outputs like bioremediation and inducible cell death.

LDL Receptor-Related Protein 5 is a Selective Transporter for Unesterified Polyunsaturated Fatty Acids

This study identifies LRP5 as a selective transporter for unesterified PUFAs, showing its role in mTORC1 inhibition and protection against myocardial injury.

Active Gαi/o mutants accelerate breast tumor metastasis via the c-Src pathway

This study shows that active Gαi/o mutants enhance breast cancer metastasis via the c-Src pathway without affecting primary tumor growth.

Spike substitution T813S increases Sarbecovirus fusogenicity by enhancing the usage of TMPRSS2.

The paper identifies the T813S mutation in the S2 subunit of SARS-CoV Spike protein enhances fusogenicity by improving TMPRSS2 cleavage, increasing viral replication.

Spatial transcriptomics reveals a conserved segment polarity program that governs muscle patterning in Nematostella vectensis

This study uses spatial transcriptomics to identify conserved segment polarity genes in Nematostella vectensis, suggesting polarized metameric structures existed in the Cnidaria-Bilateria common ancestor.

A converged ubiquitin-proteasome pathway for the degradation of TOC and TOM tail-anchored receptors

The paper identifies a shared ubiquitin-proteasome pathway for degrading TOC and TOM tail-anchored receptors in Arabidopsis thaliana.

Intramuscular injection of nerve growth factor as a model of temporomandibular disorder: nature, time-course, and sex differences characterising the pain experience

This study characterizes pain, functional impairment, and hyperalgesia from NGF injections in a TMD model, revealing sex differences in pain experience.

Hepatitis E virus genome replication is independent of cyclophilins A and B

This study investigates the role of cyclophilins A and B in HEV genome replication and finds they are not essential for replication of genotype 1 or 3 HEV, though CypB silencing reduces genotype 1 replication in some cells.

Polyvalent mRNA vaccination elicited potent immune response to monkeypox surface antigens

This study develops a polyvalent mRNA vaccine against monkeypox virus and shows it elicits strong antibody responses to key surface antigens in animal models.

High-throughput tagging of endogenous loci for rapid characterization of protein function

This paper introduces HITAG, a method for high-throughput tagging of endogenous loci to study protein function, and demonstrates its use in analyzing stress granule-associated proteins.

Network topology enables efficient response to environment in Physarum polycephalum

This study compares the avoidance response of Physarum polycephalum in I-shaped and Y-shaped forms, finding that the Y-shaped network topology allows for more efficient retraction under adverse conditions.

Mechanically manipulate glymphatic transportation by ultrasound combined with microbubbles

This study shows that ultrasound with microbubbles can enhance glymphatic transport in mouse brains by mechanically stimulating perivascular fluid movement.

GPR84-Mediated Signal Transduction Promotes Brown Adipocyte Function

This study shows that GPR84 promotes brown adipocyte function by regulating mitochondrial calcium and respiration, with implications for metabolic disease treatment.

Gating interactions steer loop conformational changes in the active site of the L1 metallo-β-lactamase

The paper investigates how gating interactions between loops in the L1 metallo-β-lactamase affect active site conformational changes and identifies key residues critical for enzyme activity.

FGFR1 governs iron metabolism via regulating post-translational modification of IRP2 in prostate cancer cells

This study shows FGFR1 promotes prostate cancer by enhancing iron metabolism through regulation of IRP2 post-translational modification and TFR1 expression.

Gene inversion triggered origination of brackish archaeal heterotrophs in the aftermath of the Cryogenian Snowball Earth

The paper traces the evolutionary origin of brackish archaea, showing gene rearrangements enabled their adaptation to coastal environments after the Cryogenian Snowball Earth.

Polylactide Degradation Activates Immune Cells by Metabolic Reprogramming

This study shows that PLA degradation products reprogram cell metabolism, leading to immune activation and inflammation, and demonstrates that targeting metabolism can reduce inflammation and improve biocompatibility.

Plant and prokaryotic TIR domains generate distinct cyclic ADPR NADase products

This study investigates whether prokaryotic Thoeris TIRs can cross-activate plant TIR-immunity and finds they generate overlapping cADPR isomers but have distinct signaling requirements.