Extracellular vesicles in disease

EVRCEPT: EV RNA Cargo Enrichment Prediction Tool to predict enrichment of RNA into Extracellular Vesicles

EVRCEPT predicts RNA enrichment in extracellular vesicles using sequence features and provides RBP interactions, aiding in therapeutic RNA design.

RNY1 partitions into extracellular vesicles and ribonucleoprotein particles during airway inflammation to regulate macrophage programming

This study identifies RNY1 as a YRNA that partitions into extracellular vesicles and ribonucleoprotein particles during airway inflammation, influencing macrophage activation through distinct mechanisms.

A meta-analysis of age-dependent changes in extracellular vesicle proteins in C. elegans

This study performs a meta-analysis of age-dependent changes in extracellular vesicle proteins in C. elegans, revealing age-related abundance changes, aggregation, and overlap with Alzheimer's disease-related proteins.

A single extracellular vesicle-based platform supporting both RBD protein and mRNA vaccination against SARS-CoV-2

This paper develops an extracellular vesicle-based vaccine platform that supports both RBD protein and mRNA vaccination against SARS-CoV-2, showing robust immune responses in mice.

Formation of extracellular vesicles depends on mechanical feedback of the cortex and the glycocalyx

This paper develops a biophysical model to study how glycocalyx and membrane-cortex adhesion influence extracellular vesicle formation, identifying two instabilities and critical conditions for EV generation.

Secreted exosomes induce filopodia formation

This study identifies exosomes carrying THSD7A as regulators of filopodia formation in both cancer cells and neurons, mediated through Cdc42 activation.

Micronuclear collapse under oxidative stress drives amphisome-mediated export of DNA in Parkinson disease

The paper shows that oxidative stress causes micronuclear collapse in Parkinson's fibroblasts, leading to DNA export via amphisomes and pro-inflammatory EVs, which can be mitigated by a mitochondria-targeted antioxidant.

Therapeutic Potential of Neural Rosette Stem Cell-Derived Small Extracellular Vesicles in the Management of Brain Edema

This study characterizes sEV cargo from NRSCs and evaluates their therapeutic effects in a rat brain edema model, showing improved cell viability and faster edema resolution.

Proteolysis targeting chimera extracellular vesicles for therapeutic development treating triple negative breast cancer

This paper demonstrates that microfluidic droplet-based electroporation (microDES) enhances PROTAC drug delivery via extracellular vesicles, improving therapeutic efficacy and safety in a TNBC mouse model.

Exosome tethering requires tetherin homodimerisation

This study identifies tetherin homodimerisation as essential for exosome tethering, showing that mutations affecting tetherin trafficking to ILVs have minimal impact on exosome retention.

Vesicular Rps6 released by astrocytes regulate local translation and enhance synaptic markers in neurons

This study shows astrocytes regulate local protein synthesis in neurons by releasing extracellular vesicles containing Rps6, enhancing synaptic markers.

Non-targeted Analysis of Extracellular Vesicle-Enriched Plasma Proteome between Early and Late Rugby Playing Career

The paper compares the plasma extracellular vesicle proteome of professional rugby players, academy players, and cross-fit athletes to identify neurodegenerative risk biomarkers.

Impact of storage on the stability and the protective effect of extracellular vesicles released by Candida albicans

This study evaluates the stability and protective effect of Candida albicans extracellular vesicles under various storage conditions and isolation methods, finding they maintain biological function but are affected by freeze-thaw cycles.

Rapid and efficient isolation of intact engineered extracellular vesicles encapsulating functional proteins via internal twin Strep tetraspanin tagging and Strep technology

The paper develops a method to isolate intact engineered extracellular vesicles with high purity using twin-Strep tagging and Strep technology, enabling efficient and selective capture and functional delivery of proteins.

Genetically-programmed Hypervesiculation of Lactiplantibacillus plantarum Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model

This study genetically engineers Lactiplantibacillus plantarum to produce 66-fold more extracellular vesicles, which show superior therapeutic efficacy in a mouse model of inflammatory bowel disease.

p62 sorts Lupus La and selected microRNAs into breast cancer-derived exosomes

The paper identifies p62 as a factor that sorts La and miR-122 into exosomes, showing its role in modulating exosomal miRNA composition and promoting tumor growth.

Red blood Cell-derived Extracellular Vesicles as biomaterials: the opportunity of freezing-induced accelerated aging

This study uses freezing-induced aging to produce homogeneous RBC-EVs, demonstrating improved functionality and storage stability for EV-based nanomedicine.

EVtrace: tracing extracellular vesicles-associated proteins in recipient cells using stable isotope labeling

EVtrace uses SILAC to trace EV-associated proteins in recipient cells, showing non-stochastic targeting of EV subpopulations to specific cell types.

Extracellular vesicles as indicators of environmental stress response in Lactiplantibacillus plantarum: a multi-platform study

This study investigates how bile exposure affects EV production and properties in Lactiplantibacillus plantarum, revealing strain-specific adaptations and molecular changes in EVs.

Serum Exosomes from a Uniquely Defined Early-Adult Cohort Reprogram Endothelial Transcriptomes Linked to Alzheimer's Pathogenesis.

The paper shows that exosomes from young adults reverse aging-related transcriptomic changes in endothelial cells linked to Alzheimer's disease.

Proteomic analysis of exosomes from lymphatic affluents reveals their implications in developing premetastatic niche in melanoma.

This study identifies unique proteomic signatures in melanoma-derived lymphatic exosomes that contribute to premetastatic niche formation in sentinel lymph nodes.

Extracellular vesicles regulate metastable phenotypes of lymphangioleiomyomatosis cells via shuttling ATP synthesis to pseudopodia and activation of integrin adhesion complexes.

This study shows that extracellular vesicles from LAM cells promote metastasis by delivering ATP to pseudopodia and activating integrin adhesion complexes.

Copper-Free Click Chemistry Enables High-Fidelity Engineering of Mitochondria Targeted Brain-Derived Exosomes

This paper uses copper-free click chemistry to engineer mitochondria-targeted brain-derived exosomes, preserving their structure and function while enabling targeted delivery and imaging.

Harnessing hydrodynamics for high-yield production of extracellular vesicles from stem cells spheroids with specific cargo profiling

This paper introduces a hydrodynamic method to produce extracellular vesicles from stem cell spheroids, showing increased yield and distinct protein cargo profiles under hydrodynamic stimulation.

Light-induced Extracellular Vesicle Adsorption

This paper introduces LEVA, a label-free, high-resolution, tunable platform for EV adsorption and characterization, demonstrating its versatility with various EV sources and applications.

Stress-induced brain extracellular vesicles ameliorate anxiety behaviour.

This study shows that stress-induced brain extracellular vesicles reduce anxiety in mice, and three miRNAs within these vesicles contribute to the anxiolytic effect by suppressing Mecp2 in neurons.

DMEM, or Opti-MEM, that is the Question: An Important Consideration for Extracellular Vesicle Isolation and their Downstream Applications.

This study compares EV release and composition in different media, finding that Opti-MEM promotes more EV release and distinct pathway usage compared to EV-DEP DMEM.

Validation of Extracellular Vesicles in FACTORFIVEs EXOFECTA LyoBeads

The paper validates the presence of extracellular vesicles in FACTORFIVEs EXOFECTA LyoBeads after flash freezing and lyophilization using multiple characterization techniques.

Investigating milk-derived extracellular vesicles as mediators of maternal stress and environmental intervention

This study shows that maternal stress during lactation alters milk-derived extracellular vesicle miRNAs, which affect offspring hippocampal gene expression, and that environmental enrichment can reverse these effects.

Tunneling nanotubes propagate preneoplastic cues in a BMP-dependent manner.

The paper shows that tunneling nanotubes (TNTs) transfer preneoplastic signals via BMP-dependent pathways in breast cells, promoting transformation in non-transformed cells.