Zebrafish Biomedical Research Applications

Phytochemical evaluation, Embryotoxicity and Teratogenic effects of Curcuma longa extract in Zebrafish (Danio rerio).

This study evaluates the embryotoxicity and teratogenic effects of Curcuma longa extract on zebrafish embryos and larvae, finding dose-dependent toxicity and physical deformities at higher concentrations.

Multi-sample SPIM image acquisition, processing and analysis of vascular growth in zebrafish

The paper presents a multi-sample imaging, processing, and analysis platform for studying vascular growth in zebrafish over time.

Chronology-based architecture of descending circuits that underlie the development of locomotor repertoire after birth.

The paper examines how new spinal pathways from hindbrain V2a neurons develop in larval zebrafish to support the emergence of diverse locomotor behaviors postnatally.

Distinct Spinal V2a and V0d Microcircuits Distribute Locomotor Control in Larval Zebrafish

The paper identifies two distinct types of V2a interneurons in larval zebrafish that distribute locomotor control through different functional roles, challenging hierarchical models of spinal circuitry.

Neurotrophin Receptor Ntrk2b Function In The Maintenance Of Dopamine And Serotonin Neurons In Zebrafish

This study investigates the role of Ntrk2b in maintaining dopamine and serotonin neurons in zebrafish, showing its specific impact on these aminergic systems.

Human macrophages survive and adopt activated genotypes in living zebrafish

This study shows human macrophages survive and adopt activated genotypes in zebrafish, mimicking cancer-related immune responses.

zGrad: A nanobody-based degron system to inactivate proteins in zebrafish

This paper introduces zGrad, a nanobody-based degron system for inducible and reversible protein inactivation in zebrafish, demonstrating its utility in studying protein function across different spatial and temporal contexts.

Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping

The paper generates and annotates new Cre transgenic zebrafish lines for intersectional genetic experiments and integrates them with a brain atlas for spatial search.

Visual intensity ratio modulates operant learning responses in larval zebrafish

This study develops a high-throughput operant learning system for larval zebrafish and shows that lower visual intensity ratios enhance learning responses and memory extinction.

Deep learning image recognition enables efficient genome editing in zebrafish by automated injections

This paper develops a deep learning system for automated microinjection in zebrafish embryos, achieving efficiency comparable to manual methods and enabling high-throughput genome editing.

Genome-wide strategies reveal target genes of Npas4l associated with cardiovascular development in zebrafish

The paper identifies Npas4l target genes involved in cardiovascular development using zebrafish models and genome-wide approaches.

Dissection of intestines from larval zebrafish for molecular analysis

The paper introduces a method to isolate larval zebrafish intestines for molecular analysis, showing that 10 and 25 intestines are sufficient for RNA-sequencing and ChIP-sequencing, respectively.

Rearranged zebrafish genomic DNA induces zebrafish mutant after microinjection into fertilized egg and preliminary study of the mechanism

This paper describes the creation of rearranged zebrafish genomic DNA through enzymatic digestion and adaptor linking, leading to red flesh mutants and analysis of the genomic and transcriptomic effects.

Dynamic control of proinflammatory cytokines Il-1β and Tnf-α by macrophages is necessary for functional spinal cord regeneration in zebrafish

The paper shows that macrophage-mediated dynamic control of Il-1β and Tnf-α is essential for spinal cord regeneration in zebrafish.

Biological motion as an innate perceptual mechanism driving social affiliation

The paper identifies biological motion as an innate perceptual cue driving shoaling in zebrafish using a virtual reality assay.

Generation of Mouse-Zebrafish Hematopoietic Tissue Chimeric Embryos for Hematopoiesis and Host-Pathogen Interaction Studies

This study xenografts murine bone marrow cells into zebrafish embryos to study hematopoiesis and host-pathogen interactions, showing successful integration and immune response.

Damage-induced reactive oxygen species enable zebrafish tail regeneration by repositioning of Hedgehog expressing cells.

This study shows that ROS-induced repositioning of Hedgehog-expressing notochord cells drives zebrafish tail regeneration, highlighting a regeneration-specific role for developmental signaling.

Acute fluoxetine differently affects aggressive display in zebrafish phenotypes

This study compares aggressive display differences between leopard and longfin zebrafish and shows varying effects of acute fluoxetine on aggression in these phenotypes.

Zebrabase: An intuitive tracking solution for aquatic model organisms

Zebrabase provides a scalable database for tracking aquatic model organisms, offering management, reporting, and visualization tools for research facilities.

Morphogenesis and differentiation of embryonic vascular smooth muscle cells in zebrafish

The paper traces the morphogenesis and transcriptome of early vascular smooth muscle cells in zebrafish using foxc1b as a marker.

Connectomics of the zebrafish’s lateral-line neuromast reveals wiring and miswiring in a simple microcircuit

The paper maps the connectome of zebrafish neuromasts, revealing specific, redundant, and hierarchical wiring patterns, and identifies Notch signaling as mediating wiring specificity.

Secretogranin-II Plays A Critical Role In Zebrafish Neurovascular Modeling

The paper shows that sgIIb is critical for hindbrain neurovascular modeling in zebrafish through MAPK and PI3K/AKT signaling.

Generation Of A Double Binary Transgenic Zebrafish Model To Study Myeloid Gene Regulation In Response To Melanocyte Transformation

The paper develops a double binary zebrafish model to study myeloid gene regulation during melanocyte transformation and melanoma initiation.

An optimized pipeline for parallel image-based quantification of gene expression and genotyping after in situ hybridization

The paper describes an optimized pipeline for genotyping zebrafish embryos after in situ hybridization and digitally quantifying gene expression, improving accuracy and reducing bias.

A brain wide circuit model of heat evoked swimming behavior in larval zebrafish

The paper develops a dynamic circuit model to explain how heat sensation is transformed into swimming behavior in larval zebrafish.

Analysis of behavioral variables across domains and strains in zebrafish: Role of brain monoamines

The paper identifies neurochemical and behavioral differences across zebrafish strains, linking behavioral traits to monoamine levels such as serotonin and dopamine.

Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

This study develops a technique to image whole brain neural activity in freely behaving larval zebrafish during specific behaviors.

A novel mechanism for volitional locomotion in larval zebrafish

This study shows larval zebrafish use independent volitional and reflexive movements for locomotion, revealing a simple control scheme that enables effective but inflexible swimming.

microCT-Based Phenomics in the Zebrafish Skeleton Reveals Virtues of Deep Phenotyping in a Distributed Organ System

This study uses microCT to analyze zebrafish skeletons, identifying phenotypes linked to human diseases and showing how deep phenotyping improves mutant discrimination.

High precision registration between zebrafish brain atlases using symmetric diffeomorphic normalization

This study aligns two zebrafish brain atlases using symmetric diffeomorphic normalization, improving registration precision and preserving morphology.