Bruno Weber

PURELIGHT: a quantitative photon-counting framework unifying intensity and lifetime imaging at video rate across detector technologies

PURELIGHT improves photon efficiency and accuracy in fluorescence microscopy by recovering undistorted intensity and lifetime measurements at high count rates across multiple detector technologies.

A longitudinal two-photon imaging platform for focal astrocyte ablation in vivo

This paper introduces an in vivo model for focal astrocyte ablation combined with longitudinal two-photon imaging to study CNS responses to astrocyte loss.

Brain pericytes exhibit spatially organized and dynamically regulated molecular heterogeneity

This study identifies spatial and dynamic molecular heterogeneity in adult mouse brain pericytes across anatomical regions, vascular positions, aging, ischemia, and circadian phases.

Opportunistic neuronal fuelling

This study shows neurons switch fuel sources from glucose to lactate when extracellular lactate increases, regardless of activity, revealing lactate availability as the key factor.

ECgo: All-Optical Induction of Single Endothelial Cell Injury and Capillary Occlusion in the Brain

The paper introduces ECgo, an optical method to induce single endothelial cell injury and capillary occlusion in the mouse brain with high spatial precision, enabling studies of capillary repair and dysfunction.

Adolescent Astrocyte Dysregulation Impairs Prefrontal Interneuron Maturation and Adult Cognition

This study shows that abnormal astrocyte activity during adolescent prefrontal cortex development leads to synaptic loss, interneuron dysfunction, and cognitive deficits.

Developmental Trajectory of Synaptic Remodeling in the Mouse Prefrontal Cortex

The paper characterizes synaptic dynamics during normal mouse prefrontal cortex maturation using combined imaging and quantification methods.

Streak-Aware Localization Microscopy Enables High-Throughput Brain Imaging Across Platforms

SALM uses deep learning to convert motion-blurred streaks into high-resolution brain imaging, reducing reconstruction time and enabling scalable, cross-platform applications.

Photoreceptors have a dual dependency on both aerobic glycolysis and OXPHOS and diverge metabolically from other retinal neurons

The paper examines energy metabolism in retinal neurons, showing photoreceptors use both aerobic glycolysis and OXPHOS while inner retinal neurons rely on OXPHOS, and identifies metabolic changes in a retinitis pigmentosa mutation.

Reprogrammed neutrophils with impaired transit mechanics drive multi-organ capillary stalling after stroke

This study identifies reprogrammed neutrophils with impaired transit mechanics as a driver of multi-organ capillary stalling after stroke, showing therapeutic potential through Fgr inhibition.

Pial collaterals propagate neurovascular responses across functional units

The paper shows pial collaterals propagate neurovascular signals between cortical territories, synchronizing blood flow responses beyond activated areas.

APOE genotype-dependent differences in human astrocytic energy metabolism

This study investigates how different APOE genotypes affect human astrocytic energy metabolism, finding reduced ATP production in APOE4 astrocytes but increased mitochondrial respiration and glycolytic capacity.

In Vivo Network-Level Cerebrovascular Mapping Reveals the Impact of Flow Topology on Capillary Stalls After Stroke

The paper develops OMNIMap to analyze cerebrovascular flow and identifies flow topology as a marker of capillary vulnerability after stroke.

A low-cost FPGA-based approach for pile-up corrected high-speed in vivo FLIM imaging

This paper introduces a low-cost FPGA-based method to correct pile-up distortions in TCSPC-FLIM, enabling high-speed, accurate in vivo imaging with subcellular resolution.

Transcranial cortex-wide imaging of murine ischemic perfusion with large-field multifocal illumination fluorescence microscopy

This paper develops a new imaging technique to study brain perfusion in mice during ischemic stroke, showing its ability to map vascular changes and assess therapeutic effects.

Overactivation of prefrontal astrocytes impairs cognition through the metabolic pathway of central kynurenines

This study shows that overactivation of prefrontal astrocytes in mice impairs cognition and PV interneuron function through increased kynurenine pathway metabolite KYNA.

Bessel Beam Optical Coherence Microscopy Enables Multiscale Assessment of Cerebrovascular Network Morphology and Function

This study introduces Bessel beam optical coherence microscopy to assess cerebrovascular network morphology and function in mice at high resolution and across multiple scales.

Lactate-carried Mitochondrial Energy Overflow

The paper investigates mitochondrial lactate metabolism, finding that mitochondria produce lactate as an overflow mechanism to regulate redox levels and conserve energy.

Pia-FLOW: Deciphering hemodynamic maps of the pial vascular connectome and its response to arterial occlusion

Pia-FLOW maps hemodynamic properties of the murine pial vascular network and shows its unified response to arterial occlusion.

Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide release

This paper develops a genetically encoded sensor, NOPLight, to detect endogenous N/OFQ release with high spatial and temporal resolution in vitro and in vivo.

Astrocytes adopt a progenitor-like migratory strategy for regeneration in adult brain

The paper shows that mature astrocytes can transform into progenitor-like cells to regenerate astrocytic networks after injury in mice.

L-Type calcium channels and TRPC3 channels shape brain pericyte calcium signaling and hemodynamics throughout the arteriole to capillary network in vivo

This study investigates the role of L-type and TRPC3 calcium channels in pericyte calcium signaling and hemodynamics across different cerebrovascular regions in vivo.

Potassium regulates axon-oligodendrocyte signaling and metabolic coupling in white matter

This study shows that oligodendrocytes use potassium signaling to regulate axonal metabolism and maintain axonal health through metabolic coupling.

Leptomeningeal collaterals regulate reperfusion in ischemic stroke

This study shows that leptomeningeal collaterals regulate reperfusion in ischemic stroke, with poor collaterals leading to hemorrhage and poor outcomes.

Pericyte remodeling is deficient in the aged brain and contributes to impaired capillary flow and structure

This study shows that pericyte loss in aged brains leads to capillary dilation, flow heterogeneity, and structural disruption, highlighting pericyte remodeling as a therapeutic target.

Myelin lipids as nervous system energy reserves

The paper shows that myelin acts as an energy reserve in the brain under glucose deprivation, supporting oligodendrocytes and axons through fatty acid oxidation.

The severity of microstrokes depends on local vascular topology and baseline perfusion

The paper uses blood flow simulations in mouse cortical microvascular networks to show that microstroke severity depends on local vascular topology and baseline perfusion.

Differential impact of GABAA receptors and gephyrin post-translational modifications on layer 2/3 pyramidal neuron responsiveness in vivo

The paper investigates how GABAA receptor subtypes and gephyrin post-translational modifications influence layer 2/3 pyramidal neuron responsiveness in mouse barrel cortex during sensory processing.

Distinct signatures of calcium activity in brain pericytes

This study identifies distinct calcium activity patterns in brain pericyte subtypes, showing differences in dynamics and responses to neuronal activity and extracellular potassium.

Rapid Reconfiguration of the Functional Connectome after Chemogenetic Locus Coeruleus Activation

This study uses chemogenetic activation of the locus coeruleus in mice combined with resting-state fMRI to show rapid changes in functional connectivity, particularly in salience and amygdala networks.