Erik A. Sahai

Substrate heterogeneity promotes cancer cell dissemination through interface roughening

This study shows that substrate disorder promotes single-cell cancer invasion, comparable to cadherin loss, by altering invasion dynamics through spatial heterogeneity.

Multi-Site Reproducibility Study of 3D High-Content Analysis with Dual-View Oblique Plane Microscopy

This study assesses the reproducibility of a 3D spheroid-based assay across four institutes using dual-view oblique plane microscopy and finds high consistency in treatment effects with low technical noise.

Intravital imaging uncovers remodelling of humanised bone marrow-like niches

The paper uses intravital imaging to study dynamic remodelling of humanised bone marrow-like niches in mice, revealing vascular network formation and collagen matrix changes.

Cooperative and antagonistic interactions between sub-clones favour the co-existence of multiple resistance mechanisms in melanoma

This study shows that cooperative and antagonistic interactions between sub-clones in melanoma allow multiple resistance mechanisms to coexist, and identifies GSK-J4 as a potential therapeutic target.

Multicellular Calcium Waves in Cancer-Associated Fibroblasts Regulate Neuronal Mimicry and Anisotropy Leading to Immune Exclusion

The paper identifies neuronal mimicry in cancer-associated fibroblasts driven by calcium waves, leading to immune exclusion, and shows that blocking this process restores immune control.

Inhibition of neutrophil degranulation by Nexinhib20 delays the development of radiation-induced pulmonary fibrosis

The paper shows that inhibiting neutrophil degranulation with Nexinhib20 delays radiation-induced pulmonary fibrosis without affecting cancer radiotherapy efficacy.

Rapid expansion of podoplanin-positive fibroblasts following radiation limits the anti-tumour CD8+ T-cell response to radiotherapy.

This study shows that radiation-induced podoplanin-positive fibroblasts limit CD8+ T-cell access to tumour cells, reducing radiotherapy effectiveness, and that disrupting PDPN signaling enhances tumour control.

Splenic granulopoiesis and S100A9 drive resistance to checkpoint inhibitors conferred by liver metastases

This study shows liver metastases reduce checkpoint inhibitor efficacy by increasing splenic granulopoiesis and S100A9hi neutrophils, which suppress T-cell responses, and demonstrates that targeting S100A9 restores efficacy.

Cell migration sculpts evolutionary dynamics favouring therapy resistance in lung cancer

This study shows that cell migration and stromal fibroblasts influence subclone competition in lung cancer, promoting therapy resistance through increased cell mixing.

VISIBLE: An imaging-driven system for sampling, biofabrication, and manipulation of complex biological models

VISIBLE is a system for real-time manipulation and bioprinting of complex in vitro models, enabling precise interventions and improving reproducibility in biomedical research.

Multimodal profiling unveils a reversible breast basal-like cell state in AKT-inhibitor resistant tumours

This study identifies a reversible basal-like cell state in AKT-inhibitor resistant triple negative breast cancer using multimodal profiling and lineage tracing.

AI4CellFate: Interpretable Early Cell Fate Prediction with Generative AI

AI4CellFate predicts cell fate from microscopy timelapses using generative AI and contrastive learning, enabling early prediction with limited annotation.

Imaging of MAP kinase dynamics reveals endocytic regulation of pulsatile signalling and network re-wiring in response to targeted therapy in EGFR-mutant non-small cell lung cancer

The paper uses single-cell imaging to show that drug-tolerant cells in EGFR-mutant lung cancer rewired ERK signaling through endocytic pathways and adhesion complexes, driven by YAP and PTPRS.

Oncogenic PIK3CA corrupts growth factor signaling specificity

The paper uses single-cell analyses to show that oncogenic PIK3CAH1047R reduces IGF1 signaling specificity and enhances EGF responses, leading to increased phenotypic heterogeneity.

Actin and Src-family kinases regulate nuclear YAP1 and its export

This study identifies mechanisms regulating YAP1 nuclear shuttling in fibroblasts, showing that Src and actomyosin suppress YAP1 export, and nuclear export is central to YAP1 regulation.