DNA Repair Mechanisms

This study identifies a novel transgenerational DNA repair mechanism in the asexual bdelloid rotifer Adineta vaga that uses meiotic recombination to repair fragmented chromosomes across generations.

The paper compares DNA combing and spreading techniques to determine their ability to resolve sister chromatids during DNA replication, finding that DNA combing can detect strand-specific alterations while DNA spreading cannot.

This study shows that loss of Aag in mice reduces DNA damage and alters gene expression in the brain, leading to reduced anxiety and improved memory.

The paper shows EXO1-mediated ssDNA gap expansion is critical for ATR activation and cell viability in BRCA1-deficient cells, revealing a therapeutic target for BRCA1-mutant cancers.

The paper reveals an asymmetric response of DNA damage tolerance mechanisms to leading and lagging strand polymerase stalling.

The paper shows BRCA2 stabilizes RAD51 filaments for fork protection and gap suppression, but HDR deficiency primarily drives chemotherapeutic sensitivity.

The paper investigates the temporal and functional relationship between synaptonemal complex (SC) morphogenesis and recombination during meiosis in budding yeast, showing SC's role in regulating recombination transitions.

This study shows PSIP1 reduces R-loops at transcription sites, preventing DNA damage and maintaining genome integrity in human and mouse cells.

The paper shows that NONO relocalizes to the nucleolus via diNARs to shield DNA breaks from aberrant transcripts, promoting repair.

This study identifies FANCJ's interaction with AND-1 as critical for replisome recruitment and genome stability, showing that mutations in the CIP box impair this function and contribute to cancer risk.

This study uses single-molecule imaging in E. coli to show that RecB facilitates efficient DNA repair without activating the SOS response, while a modified RecB leads to less efficient repair and high SOS induction.

The paper identifies Ku-binding motifs in RAG2, XLF, PAXX, and MRI that mediate their interactions with Ku and support functional redundancy during V(D)J recombination.

This study shows that PCNA-K164 ubiquitination is essential for DNA gap filling during S/G2 phases and regulates mitotic DNA synthesis by influencing FANCD2 and MCM2-7 loading.

This study identifies a naturally occurring MBD4 variant in rhesus macaques that causes maternal germline hypermutation, leading to increased de novo mutations in offspring.

The paper shows that ATR and DDK control TopBP1-mediated locking of dormant origins at the pre-IC stage, with compensation involving both passive and active processes.

The paper shows that the SOSS1 complex and RNA polymerase II form phase-separated compartments at double-strand breaks to facilitate DNA repair.

This study identifies the SMC5/6 complex as essential for genome stability in cells with active APOBEC3A, showing that its loss leads to increased DNA damage and replication stress.

This study identifies how Mec1/ATR kinase controls homologous recombination to suppress gross chromosomal rearrangements through Rad53 and Sgs1 pathways.

This study investigates the role of the ANKRD31-REC114 interaction in meiotic recombination and spermatogenesis, showing its critical importance for DSB formation, repair, and location in mice.

This study identifies GNL3 as a protein that prevents DNA resection at stalled replication forks, highlighting its role in protecting the genome from damage during replication stress.

This study identifies catalytic and non-catalytic functions of DNA polymerase kappa in translesion DNA synthesis using CRISPR screening and TLS analysis.

This study characterizes the structure and function of TOPOVIBL, a subunit of the TOPOVIL complex involved in meiotic DSB formation, revealing its DNA interaction properties and dynamic conformation.

The paper shows that DNA double-strand breaks near inverted repeats drive inverted duplications in Sae2 and Mre11-deficient cells through a mechanism involving DNA polymerase delta and Rad51.

The paper shows that HMCES-DNA protein crosslinks are resolved through self-reversal, which is crucial for managing ssDNA abasic sites and maintaining genome stability.

This study shows 53BP1 interacts with RNA primers from Okazaki fragments during DNA replication, supporting their processing and highlighting an RNA-protein interaction at replication forks.

The paper introduces CC-seq, a method to map Spo11 DNA double-strand breaks with nucleotide-resolution in S. cerevisiae meiotic cells.

This study shows FIGNL1 regulates RAD51/DMC1 filament dynamics in meiotic recombination by dismantling non-productive assemblies during pre-meiotic S-phase and meiotic prophase I.

This study shows PKCδ regulates DNA repair and chromatin structure through SIRT6, affecting genomic stability and radiation-induced apoptosis.

The paper induces DSBs in Arabidopsis using a heat-inducible restriction enzyme and analyzes the chromatin remodeling dynamics of H4K16ac and H2A.Z at these sites.

The paper shows that CTCF/cohesin binding during DNA replication causes replication stress and genomic instability at CBSs in cancer.