Advanced biosensing and bioanalysis techniques

A Master-Key DNA System Enabling Programmable Cross-Talks in Biomimetic Networks via An Artificial Chaperone

An EpCAM-Targeted Mirror-Image DNA Nanostructure for Precise Drug Delivery in Triple-Negative Breast Cancer

This paper develops an EpCAM-targeted L-DNA nanostructure for targeted doxorubicin delivery in TNBC, showing improved stability and reduced cytotoxicity in normal cells.

Resolving Sub-Microsecond Conformational Dynamics of Vertical Nucleic Acids on Graphene

This study observes sub-microsecond dynamics of single DNA molecules using graphene-based fluorescence measurements and links findings to structural and environmental factors.

Strand Displacement Activity of Mimiviral Polymerase X Enables Rapid Detection of Sequence-Specific DNA Targets

The paper demonstrates a DNA detection method using Mimiviral polymerase X (mvPolX) that is sequence-specific and sensitive, capable of detecting as few as 10 copies of target DNA in 15 minutes.

A Nanoscale Jitterbug Transformer from DNA

This paper designs a DNA-based nanoscale Jitterbug transformer that reconfigures its structure in response to chemical and optical signals, maintaining a Poissons ratio of -1.

Programmable Nucleic Acid Sensing in Human Cells Using Circularizable ssDNA

This paper introduces SONAR, a platform for detecting nucleic acids and triggering gene expression in human cells, demonstrating its ability to sense DNA and RNA and express various protein payloads.

FINDER converts zero-background kinetic fingerprinting into area-scalable attomolar biomarker detection

FINDER enables attomolar biomarker detection by converting kinetic fingerprinting into area-scalable sensitivity without amplification.

Bayesian optimisation and graph-based rheology enable sequence-dependent modelling of DNA materials

This paper uses Bayesian optimisation and graph-based rheology to model DNA materials by linking sequence design to material properties.

DNA condensate-based organelles for spatially regulated gene expression and protein targeting in synthetic cells

The paper demonstrates DNA condensate-based organelles that enable spatially regulated gene expression and protein targeting in synthetic cells, showing higher protein yield compared to standard PURE expression.

Biofunctional 2D Graphitic Carbon Nitride-Hydrogel Heterointerfaces for Electrochemical Detection of Interleukin-6 toward Septic Cardiomyopathy Diagnostics in Clinical Biofluids

This paper develops an electrochemical aptasensor for ultrasensitive detection of IL-6 in clinical biofluids, showing high sensitivity and biocompatibility for septic cardiomyopathy diagnostics.

Expanding High-Fidelity Multiplexing in Ultrasensitive Single-Molecule Protein Detection via Proximity Barcoding

This paper introduces PRO-MOSAIX, a high-accuracy, ultrasensitive multiplex immunoassay that enables 15-plex detection in human plasma with low femtomolar sensitivity.

Adverse Effects of UV-Exposure on DNA Strand Displacement Reactions

This study identifies UV-shadowing as a source of errors in DNA strand displacement reactions and proposes a UV-free purification protocol to improve circuit performance.

Screening Molecular Recognition Element-Based SWCNT Optical Sensors for the Inflammatory Cytokine TNF-α

This study evaluates aptamer and antibody-based sensors for detecting TNF-α in serum using SWCNT optical sensors, finding that modifications improve sensitivity and selectivity.

Monitoring in vivo transcription with synthetic serum markers

This paper introduces a platform called INTACT that non-invasively monitors specific mRNA levels in vivo by using synthetic serum markers and RNA-based sensors.

Aptamer charge-amplified field-effect transistor biosensors achieve picomolar detection limits for small-molecule biomarkers in complex biological matrices

This paper develops a charge-amplified FET biosensor for detecting small-molecule biomarkers with picomolar sensitivity in complex biological samples.

A 3D printed mini-gel electrophoresis system for rapid and inexpensive DNA nanoswitch biosensing

This paper presents a 3D-printed mini-gel electrophoresis system for DNA nanoswitch biosensing, achieving rapid, low-cost, and portable detection of target molecules.

Ligation-assisted target recycling for DNA nanoswitch biosensors

This paper introduces a method to enhance DNA nanoswitch biosensor sensitivity by recycling targets through enzymatic ligation and cyclic annealing, enabling signal amplification without target amplification.

Falafel-Style Wrapping of AuNPs for DNA Origami Barcodes

This paper introduces a method to encapsulate gold nanoparticles within DNA origami tubes to improve barcode stability and functionality for multiplexed biomolecular detection.

Design of DNA Aptamers for Lyme disease Diagnosis Combining experimental and numerical approaches

The paper designs DNA aptamers for Lyme disease detection by combining in silico selection using a Restricted Boltzmann Machine with experimental validation of selected sequences.

Influence of Physicochemical Parameters on the in vitro Stability of DNA Tetrahedral Nanostructures

This study evaluates how physicochemical factors affect the stability of DNA tetrahedral nanostructures in vitro.

Homogeneous antibody-DNA-conjugates using unmodified oligonucleotides and photo-crosslinkable protein G-HUH endonuclease fusion proteins

This paper introduces a method to create homogeneous antibody-DNA conjugates using unmodified oligonucleotides and photo-crosslinkable protein G-HUH endonuclease fusion proteins, achieving 1:1 stoichiometry.

Fluorogenic Aptamer Optimizations on a Massively Parallel Sequencing Platform

This paper optimizes a DNA-based fluorogenic aptamer (D-FAP) using a screening-and-simulation pipeline on a repurposed sequencing platform, achieving improved fluorescence properties.

Programmable DNA Origami Caps for Site-Selective Functionalization of Microtubule Tips and Lattice Defects

This paper develops DNA origami caps to selectively functionalize microtubule tips and lattice defects, enabling controlled cargo capture and dynamic visualization.

A critical assessment of aptamer and CRISPR-Cas12a-based biosensors for small molecule detection

This study evaluates the effectiveness of aptamer-Cas12a biosensors for small molecule detection, finding limited success and highlighting the need for aptamer validation.

NI2+ AND ZN2+-BINDING DNA MOTIFS REVEALED IN DNA APTAMERS TO AFRICAN SWINE FEVER VIRUS

This study identifies DNA aptamers that bind ASFV and show antiviral activity, with Ni2+ enhancing their affinity by 100-fold through a non-canonical DNA motif.

In vitro evolution of DNA operators enables multivalency protein-DNA interactions: towards programmable transcription factor regulation

This paper evolves DNA operators to enhance protein-DNA interactions for better transcriptional control in cell-free systems.

Structure-Guided Biochemical Design of DNA Tweezers As A Dual Target of the Primary Glioblastoma Biomarkers S100A4 and Midkine

This study designs DNA tweezers that selectively bind S100A4 and midkine, biomarkers of glioblastoma invasiveness, using computational methods and molecular docking.

Global Quantitative Analysis of Ligation Reactions in Self-Assembled DNA Nanostructures at the Single-Nick Level

The paper quantitatively analyzes individual ligation reactions in DNA origami nanostructures using qPCR, revealing edge preference and solvent-dependent behavior.

Probing Molecular Diversity and Ultrastructure of Brain Cells with Fluorescent Aptamers

This paper introduces CAptVE, a method for fluorescent aptamer-based labeling that enables molecular analysis in ultrastructurally reconstructable electron micrographs.

Identification of ICAM-1-targeting DNA aptamers as a host-directed strategy to inhibit Human Rhinovirus infection

This paper identifies DNA aptamers targeting ICAM-1 to inhibit HRV infection by blocking viral entry, showing concentration-dependent antiviral effects in vitro.