Microbial Natural Products and Biosynthesis

Drift, selection and convergence in the evolution of a nonribosomal peptide

The paper studies the evolution of nonribosomal peptides in entomopathogenic bacteria, finding evidence of both neutral drift and natural selection driving structural convergence and toxicity diversification.

Tryptophan Chemistry Driven by a Widespread Cytochrome P422 Enzyme Family

The paper identifies a widespread cytochrome P422 enzyme family that performs four novel tryptophan transformations, revealing new enzymatic capabilities and introducing a computational tool for HBP discovery.

Establishing Conserved Biosynthetic Gene Clusters of the Phylum Myxococcota

The paper identifies five conserved biosynthetic gene clusters in Myxococcota using pan-genome analysis of 195 genomes, revealing ecological roles for their metabolites.

Molecular characterisation of the acyltransferase-acyl carrier protein interface in a fungal highly reducing polyketide synthase.

The paper characterizes the AT:ACP interface in a fungal polyketide synthase using mutagenesis, mass spectrometry, and simulations to reveal structural and functional insights.

The Biosynthetic Pathway to the Pyrroloiminoquinone Marine Natural Product Ammosamide C

This paper identifies the biosynthetic pathway for ammosamide C, revealing novel enzymatic steps and roles for PEARLs beyond traditional PTMs.

Metabolic engineering and late-stage functionalization expand the chemical space of the antimalarial premarineosin A

This study uses metabolic engineering and late-stage functionalization to expand the chemical diversity of the antimalarial compound premarineosin A, identifying potent analogs.

Tiarins, a diverse family of natural Trojan-horse aminoacyl-tRNA synthetase inhibitors discovered by genome mining

The paper identifies TFX as an antibiotic that inhibits arginyl-tRNA synthetase by acting as a Trojan-horse molecule, revealing it belongs to a broader family of natural inhibitors targeting various tRNA synthetases.

GENOMIC ANALYSIS OF THE STREPTOMYCES SP. LV42-5 ISOLATED FROM INDUSTRIAL MINE DUMPS IN SHEPTYTSKYI

This study reports the whole-genome sequencing and analysis of a novel Streptomyces strain isolated from a mine dump, revealing its heavy metal resistance and potential for bioremediation and drug discovery.

LsrL modulates Lsr2-induced chromatin structure to tune biosynthetic gene cluster regulation in Streptomyces venezuelae

This study investigates how Lsr2 and LsrL regulate chromatin structure and biosynthetic gene clusters in Streptomyces venezuelae using multi-omics approaches.

Streptavidins play a multifunctional role within a biotin-pathway antibiotic network encoded in a biosynthetic supercluster

The paper reveals streptavidin's dual role in sequestering biotin and neutralizing acidomycin, balancing an antibiotic network in microbial competition.

Nurture over nature in new Micromonospora spp. strains metabolism: multi-omics analyses reveal fermentation conditions as the dominant driver over phylogeny

This study investigates Micromonospora strains' biosynthetic potential through multi-omics analysis, finding that culture conditions strongly influence metabolic profiles over phylogeny.

Structural and mechanistic insights into bacterial hydrazine biosynthesis

This study determines the crystal structures of bacterial hydrazine synthetases and elucidates their mechanism, enabling the expansion of hydrazine biosynthesis through novel enzyme discovery and chemoenzymatic synthesis.

Characterization of a novel fatty acid-modifying pathway toward the biosynthesis of tambjamine BE-18591 in Streptomyces

This study characterizes four novel Streptomyces proteins involved in the biosynthesis of the alkylamine component of the tambjamine BE-18591, revealing distinct biosynthetic mechanisms compared to Proteobacteria.

Norditerpene natural products from subterranean fungi with anti-parasitic activity

This study identifies two norditerpene lactones from subterranean fungi with potent anti-parasitic activity against Cryptosporidium parvum and Toxoplasma gondii, with low cytotoxicity and in vivo efficacy.

High-Yield Production of Cytotoxic Actinomycin D from Endophytic Streptomyces parvulus AL036 through Nutritional Optimization

This study isolates Streptomyces parvulus AL036 from Alpinia galanga roots, optimizes its growth medium to increase actinomycin D production, and demonstrates its cytotoxic effects on cancer cell lines.

Discovery of late intermediates in methylenomycin biosynthesis active against drug-resistant Gram-positive bacterial pathogens

This study identifies key biosynthetic enzymes in methylenomycin production and discovers more potent intermediates active against drug-resistant Gram-positive bacteria.

S9 Protease WprP Catalyzes Uniform and Sequential Cleavage on the Precursor Peptide in RiPP Biosynthesis

The paper identifies WprP2 as a serine protease that uniformly and sequentially cleaves the precursor peptide WprA2 in RiPP biosynthesis, expanding the known functions of S9 proteases.

Large-scale insights into the biosynthetic potential of the Bacillus cereus group

The paper analyzes the biosynthetic potential of the Bacillus cereus group using genomic data, identifying many novel biosynthetic gene clusters.

High-throughput engineering and modification of non-ribosomal peptide synthetases based on Golden Gate assembly

This paper describes a Golden Gate assembly method for high-throughput engineering of non-ribosomal peptide synthetases (NRPS) to generate novel peptides and modify natural products.

A ubiquitous Streptomyces biosynthetic megacluster encodes an arsenal of synergistic biotin-targeting antibiotics

The paper identifies a conserved Streptomyces biosynthetic megacluster producing biotin-targeting antibiotics, effective against multidrug-resistant E. coli.

Integrating genomics and metabolomics to accelerate the discovery of anti-MRSA natural products from the endophytic fungus Neocucurbitaria sp. VM-36

The paper integrates genomics and metabolomics to identify anti-MRSA natural products from the endophytic fungus Neocucurbitaria sp. VM-36, discovering a phomasetin analog with potent bactericidal activity against S. aureus.

Isolation and genomic analysis of secondary metabolism in cave Actinomycetota

The paper isolates and genomes nine Actinomycetota from cave environments, revealing their secondary metabolism diversity and taxonomic distribution.

LassoESM: A tailored language model for enhanced lasso peptide property prediction

This study develops LassoESM, a language model tailored for lasso peptide property prediction, showing improved performance in substrate compatibility and biological activity prediction.

Parallel Evolution of a Drimenol Biosynthetic Gene Cluster Unique to Marine Bacteria

The paper investigates the biosynthesis of drimenol in marine bacteria, identifies key genes and regulatory elements, and reveals parallel evolution of drimenol pathways in bacteria compared to plants and fungi.

A versatile enzymatic pathway for modification of peptide C-termini

The paper reconstitutes daptide biosynthesis in vitro to reveal enzymatic requirements and demonstrates broad substrate tolerance for modifying peptide C-termini.

Bioinformatics-based stereochemical elucidation of MM 46115: an unusual antiviral spirotetronate that inhibits clathrin-mediated endocytosis

This paper identifies and characterizes pellemicin, a spirotetronate with antiviral activity against influenza A and B viruses by inhibiting clathrin-mediated endocytosis.

Anticancer potential of soil-associated actinobacterium DHE 6-7 isolated from Enggano Island, Indonesia

This study evaluates the anticancer activity of DHE 6-7, an actinobacterium from Enggano Island, and identifies Actinomycin-D as a key metabolite, with synergistic effects when combined with 5-FU.

Overcoming Biosynthetic Limitations to Enhance Bacterial Polyketide Production

This study enhances bacterial polyketide production by optimizing medium composition and engineering enzymes, showing medium optimization is more effective than enzyme mutation.

Tectonic setting shapes microbial biosynthetic potential across global geothermal environments

The paper analyzes microbial biosynthetic potential across geothermal environments, finding that tectonic settings influence the diversity and types of biosynthetic gene clusters.

The genome assembly and detection of biosynthetic gene clusters among four novel microbial isolates from deep subsurface rock biofilms

This paper reports the genome assemblies and biosynthetic gene cluster analysis of four deep subsurface microbial isolates, identifying potential natural product pathways and shared/strain-specific enzymes.