Glycosylation and Glycoproteins Research

Ratios in Disguise, Truths Arise: Glycomics Meets Compositional Data Analysis

This paper introduces a compositional data analysis framework for glycomics to address statistical issues in comparative glycan data, reducing false positives and revealing new biological insights.

Immunoglobulin A Carries Sulfated N-glycans Primarily at the Tailpiece Site -- An Oxonium-Ion-Guided Approach for Site-Specific N-glycan Identification

This study identifies sulfated and O-acetylated N-glycans at specific sites in human IgA using a targeted N-glycoproteomic approach, revealing their potential as biomarkers and therapeutic targets.

Global view of domain-specific O-linked mannose glycosylation in glycoengineered cells

The paper maps O-Man glycoproteins in human cells and identifies substrate specificities of O-Man glycosylation pathways.

Neuronal activity-driven O-GlcNAcylation promotes mitochondrial plasticity

This study shows that neuronal activity increases mitochondrial O-GlcNAcylation, which helps neurons meet energy demands based on fuel availability.

Enrichment of glycoRNAs using galactose oxidase, hydrazide chemistry, and glycosidase digestion

The paper develops and optimizes methods to enrich and identify glycoRNAs, demonstrating efficient digestion and preservation techniques for studying their role in immune responses and disease.

The human and mouse dendritic cell receptor DCIR binds to LRP1 N-glycans containing terminal galactoses, including the α-Gal antigen

This study identifies LRP1 N-glycans with terminal galactoses as ligands for DCIR, revealing its specificity in immune regulation and potential therapeutic applications.

Decoding glycosylation potential from protein structure across human glycoproteins with a multi-view recurrent neural network

The paper trains a hybrid neural network to predict N-glycosylation events and structures from protein sequences using glycosite-flanking data, demonstrating its ability to predict glycan composition and abundance.

Protein structure, a genetic encoding for glycosylation

The paper identifies a relationship between protein structure and glycan structure, introducing a glycoimpact matrix to predict glycosylation patterns and validate it through disease variants and engineered antibodies.

O-GalNAc glycans enrich in white matter tracts and regulate nodes of Ranvier

The paper identifies O-GalNAc glycans' spatial enrichment in white matter and their role in regulating nodes of Ranvier through glycoproteomic analysis and functional experiments.

A Boltzmann model predicts glycan structures from lectin binding

This paper uses a Boltzmann model to predict glycan structures from lectin binding data, achieving 88% accuracy for N-glycans and 87% for O-glycans in a test set.

GPnotebook: A pan-cancer glycoproteomic database and toolkit for analysis of protein glycosylation changes associated with cancer phenotypes

The paper introduces GPnotebook, a glycoproteomic database and toolkit analyzing cancer-associated glycosylation changes using data from CPTAC.

Human brain glycoform co-regulation network and glycan modification alterations in Alzheimer's disease

This study identifies over 10,000 N-glycoforms in human brain and finds reduced sialylation and N-glycan branching, along with increased mannosylation and truncation in Alzheimer's disease.

Neurodevelopmental defects in a mouse model of O-GlcNAc transferase intellectual disability

This paper describes mouse models with OGT mutations that exhibit neurodevelopmental defects, including microcephaly and cognitive impairments, to study OGT-CDG mechanisms and potential treatments.

Combinatorial approach with mass spectrometry and lectin microarray dissected glycoproteomic features of virion-derived spike protein of SARS-CoV-2

The paper identifies site-specific glycan compositions of SARS-CoV-2 spike protein using mass spectrometry and lectin microarray, revealing key glycan structures relevant to vaccine design.

GlycansToGraphs: visualizing and simplifying complex mass spectra

GlycansToGraphs is a tool that identifies and visualizes mass relationships in glycopeptide spectra without requiring databases.

N-glycosylation as a eukaryotic protective mechanism against protein aggregation

This study shows N-glycosylation prevents protein aggregation by steric hindrance, especially in proteins prone to misfolding, using computational and biochemical analyses.

Perturbing nuclear glycosylation in the mouse preimplantation embryo slows down embryonic growth

This study removes O-GlcNAc from mouse preimplantation embryos and finds it slows embryonic growth without affecting genome activation.

The genetics and epidemiology of N- and O- IgA glycomics

This study presents the first large-scale IgA glycomics dataset from twins, identifies shared and specific genetic loci influencing IgA and IgG glycosylation, and shows genetic conservation across ethnicities.

Syntactic Sugars: Crafting a Regular Expression Framework for Glycan Structures

The paper introduces a regular expression framework for glycan structures to automate analysis of glycan sequences and annotate motifs, enabling differential expression and lectin binding specificity studies.

Computationally guided insights into substrate recognition and specificity of Alg1

This study reveals the substrate recognition and specificity mechanisms of Alg1 using molecular dynamics simulations and mutant assays.

Mapping of the gene network that regulates glycan clock of ageing

The paper identifies 16 genomic loci associated with IgG galactosylation through GWAS and validates three genes using CRISPR/dCas9 in an in vitro system.

Systems Analysis Deciphers Transcriptional Regulation and Glycosylation Links in Alzheimer's Disease

The paper identifies dysregulation of PLOD3 in oligodendrocytes as an upstream regulator of COL4A5, linked to amyloid fiber formation in Alzheimer's disease.

Human-specific features and developmental dynamics of the brain N-glycome

The paper characterizes the evolution of the brain N-glycome across primates, finding increased complexity and human-specific sialic acid linkage changes.

New avenues for human blood plasma biomarker discovery via improved in-depth analysis of the low-abundant N-glycoproteome

The paper develops an improved N-glycoproteomic workflow to analyze low-abundant glycoproteins in human blood plasma, enabling the identification of 1,929 N-glycopeptides and 942 N-glycosites.

EOGT Enables Residual Notch Signaling in Mouse Intestinal Cells Lacking POFUT1

The paper shows that EOGT and O-GlcNAc glycans provide residual Notch signaling, enabling viability in mice lacking Pofut1 in the intestine.

N-glycans show distinct spatial distribution in mouse brain

The paper identifies distinct spatial distributions of N-glycans in the mouse brain using lectin-based staining.

Neuroectoderm phenotypes in a human stem cell model of O-GlcNAc transferase intellectual disability

This study investigates OGT-CDG using stem cells, finding disrupted O-GlcNAc homeostasis and morphology during neuroectoderm differentiation but preserved stemness and differentiation potential.

A draft of human N-glycans of glycoRNA

The paper reports a draft of human tissue N-glycans of glycoRNA across 12 organs using mass spectrometry-based N-glycomics.

Deep quantitative glycoproteomics reveals gut microbiome induced remodeling of the brain glycoproteome

The paper introduces a high-throughput glycoproteomics method to quantify brain glycoproteome changes induced by gut microbiome composition.

Plasma glycoproteomics delivers high-specificity disease biomarkers by detecting site-specific glycosylation abnormalities

The paper develops a plasma N-glycoproteomics method to identify high-specificity disease biomarkers by detecting site-specific glycosylation abnormalities in genetic diseases.