Glycosylation and Glycoproteins Research

Functional Organization of Glycolytic Metabolon on Mitochondria

This study identifies OGT-mediated O-GlcNAcylation of HK1 as a mechanism regulating glycolytic metabolon assembly on mitochondria, enhancing ATP production and synaptic function.

Decoding Glycomics: Differential Expression Reimagined

This paper introduces a computational framework for differential glycomics expression analysis, demonstrating improved robustness and reliability through specialized methods and an open-source tool.

GlycoDraw: A Python Implementation for Generating High-Quality Glycan Figures

GlycoDraw generates high-quality, SNFG-compliant glycan figures in Python, enabling automated annotation and integration into glycan analysis workflows.

RAMZIS: a bioinformatic toolkit for rigorous assessment of the alterations to glycoprotein structure that occur during biological processes

RAMZIS is an R package that improves the interpretation of glycoproteomics data by assessing the significance of glycosylation changes using similarity metrics and quality evaluation.

The GlycoPaSER prototype as a real-time N-glycopeptide identification tool based on the PaSER parallel computing platform

The paper introduces GlycoPaSER, a real-time glycopeptide identification tool that decomposes spectra and uses specialized algorithms for real-time analysis, matching performance with existing software.

Glia-neuron coupling via a bipartite sialylation pathway promotes neural transmission and stress tolerance in Drosophila

The paper identifies a bipartite sialylation pathway in Drosophila where glia and neurons perform distinct roles in regulating neural transmission and stress tolerance.

An O-GlcNAc transferase pathogenic variant that affects pluripotent stem cell self-renewal

The paper shows that an OGT variant causes reduced pluripotent stem cell self-renewal and lower stem cell marker levels in mouse embryonic stem cells.

Preparing glycomics data for robust statistical analysis with GlyCompareCT

GlyCompareCT preprocesses glycomics data to address sparsity and non-independence, improving statistical power for downstream analysis.

Cell-free expression and characterization of multivalent rhamnose-binding lectins using biolayer interferometry

This paper presents a cell-free expression method for multivalent rhamnose-binding lectins and demonstrates their direct analysis using biolayer interferometry for binding affinity and specificity.

Glycoproteome remodelling and granule-specific N-glycosylation accompany neutrophil granulopoiesis

The paper maps N-glycoproteome changes during neutrophil granulopoiesis, showing granule-specific glycosylation and remodelling driven by protein expression shifts.

Exome sequencing reveals aggregates of rare variants in glycosyltransferase and other genes influencing immunoglobulin G and transferrin glycosylation

The paper uses exome sequencing to identify rare variants in glycosyltransferase genes affecting transferrin and IgG glycosylation, linking them to disease-related traits.

GlyLES: Grammar-based Parsing of Glycans from IUPAC-condensed to SMILES

GlyLES converts IUPAC-condensed glycan notation to SMILES using a grammar-based approach, enabling atomic-level representation and applications in molecular modeling and machine learning.

Cell-specific Bioorthogonal Tagging of Glycoproteins

The paper introduces BOCTAG, a method to label glycoproteins in specific cell types within mixed cultures, enabling analysis of cell-specific glycosylation.

Discovery of a MUC3B gene reconstructs the membrane mucin gene cluster on human chromosome 7

The paper identifies a previously unmapped MUC3B gene within a conserved membrane mucin gene cluster on human chromosome 7 and shows its exclusive expression in intestinal epithelial cells.

A genome-wide CRISPR screen identifies the glycosylation enzyme DPM1 as a modifier of DPAGT1 deficiency and ER stress

The paper identifies DPM1 as a therapeutic target for DPAGT1-CDG by showing its inhibition rescues ER stress and glycoprotein levels in Drosophila models.

Deep Learning Explains the Biology of Branched Glycans from Single-Cell Sequencing Data

The paper uses deep learning to predict branched glycan phenotypes from single-cell transcriptomic data, identifying key genes influencing glycan biology.

Simple and practical sialoglycan encoding system reveals vast diversity in nature and identifies a universal sialoglycan-recognizing probe derived from AB5 toxin B subunits

The paper introduces a 9-digit code system for sialyltrisaccharides, revealing vast sialoglycan diversity and identifying a universal probe from AB5 toxin B subunits.

This is GlycoQL

This paper introduces GlycoQL, a query language for glycan structure search, and demonstrates its use in analyzing SARS-CoV-2 spike protein glycosylation.

Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome

The paper develops sialoglycan-recognizing probes (SGRPs) to systematically study mammalian sialoglycome dynamics using established laboratory techniques.

Highly fucosylated N-glycans at the synaptic vesicle and neuronal plasma membrane

The paper characterizes N-glycosylation at synaptic vesicles and neuronal plasma membranes in mouse brain, identifying highly fucosylated N-glycans enriched in synaptic vesicles.

A mutant bacterial O-GlcNAcase visualizes a progressive decline of protein O-GlcNAcylation in early Drosophila embryos critical for neurodevelopment

The paper develops a fluorescent probe to visualize O-GlcNAcylation in Drosophila embryos, showing its decline during early development and its role in neurodevelopmental gene regulation.

OxoScan-MS: Oxonium ion scanning mass spectrometry facilitates plasma glycoproteomics in large scale

OxoScan-MS enables sensitive and scalable glycoproteome profiling of plasma samples, revealing differential glycosylation in severe COVID-19 patients.

JiangShi: a widely distributed Mucin-like protein essential for Drosophila development

This paper identifies JiangShi (JS), a Mucin-like protein in Drosophila, and shows its essential role in development through loss and over-production studies.

Changes in Tissue Protein N-Glycosylation and Associated Molecular Signature Occur in the Human Parkinsonian Brain in a Region-Specific Manner

The paper characterizes changes in N-glycosylation and related molecular signatures in the Parkinsonian human nigro-striatal region, linking them to endoplasmic reticulum stress and potential therapeutic implications.

Specific N-glycans regulate an extracellular adhesion complex during somatosensory dendrite patterning

The paper shows that specific N-glycans regulate an extracellular adhesion complex involved in somatosensory dendrite patterning in C. elegans.

Aberrant Fucosylation of Saliva Glycoprotein Defining Lung Adenocarcinomas Malignancy

The paper identifies increased fucosylation patterns in saliva glycoproteins of lung cancer patients, suggesting potential for early diagnosis of lung adenocarcinoma.

The homodimeric structure of the LARGE1 dual glycosyltransferase

This paper determines the homodimeric structure of LARGE1, a glycosyltransferase, revealing its dimerization mechanism and how disease mutations affect its function.

CarboGrove: a resource of glycan-binding specificities through analyzed glycan-array datasets from all platforms

CarboGrove integrates and analyzes glycan-array data from multiple sources to provide insights into glycan-binding specificities and support experimental and computational research.

Same role but different actors: genetic regulation of post-translational modification of two distinct proteins

The paper identifies genetic loci regulating glycosylation of transferrin and IgG, finding both shared and protein-specific associations and proposing tissue-specific transcription factor regulation.

A systematic comparison of current bioinformatic tools for glycoproteomics data

This study compares glycoproteomics search algorithms, finding that O-Pair excels in O-glycopeptide identification while Byonic and pGlyco perform best for N-glycoproteomics.