Glycosylation and Glycoproteins Research

Identification and comparison of N-glycome profiles from common dietary protein ingredients

The paper identifies and compares N-glycan structures from various dietary protein sources, showing differences in glycan composition based on host organism and biosynthetic pathways.

Spatiotemporal control of subcellular O-GlcNAc signaling using Opto-OGT

This study introduces Opto-OGT, an optogenetic tool to control O-GlcNAc modification, showing its ability to modulate mTORC and AKT signaling in response to light.

Substrate-directed control of N-glycosylation in the endoplasmic reticulum calibrates signal reception at the cell-surface

The paper identifies an ER pathway that regulates N-glycosylation by modulating OST-A activity, impacting cell-surface signaling and tissue development.

Quantification and site-specific analysis of co-occupied N- and O-glycopeptides

This study quantifies co-occupancy of N- and O-glycopeptides in mucins and other glycoproteins, showing higher prevalence in mucins and the need for electron-based methods for accurate site-specific analysis.

Core Tri-fucosylation of Nematode N-glycans Requires Golgi α-mannosidase III Activity that Impacts Animal Growth and Behaviours

This study identifies AMAN-3 as a Golgi α-mannosidase essential for tri-fucosylation of nematode N-glycans, which affects growth and behavior in C. elegans.

A drug repurposing screen reveals dopamine signaling as a critical pathway underlying potential therapeutics for the rare disease DPAGT1-CDG

A drug repurposing screen in a Drosophila model of DPAGT1-CDG identified dopamine D2 receptor inhibition as a potential therapeutic strategy.

Rescuable sleep and synaptogenesis phenotypes in a Drosophila model of O-GlcNAc transferase intellectual disability.

This study identifies synaptogenesis and sleep stability defects in a Drosophila model of OGT intellectual disability and shows partial rescue by modulating OGA activity.

The crystal structure of Nictaba reveals its carbohydrate-binding properties and a new lectin dimerization mode

This paper reports the crystal structure of Nictaba, revealing its carbohydrate-binding properties and a novel lectin dimerization mode.

The restricted N-glycome of neurons is programmed during differentiation

The paper investigates the N-glycome changes during neuronal differentiation and finds that brain-specific N-glycans like Man-5 are programmed independently of the transcriptome.

Aberrant glycosylation reveals unexpected clinical outcomes between Luminal B and Basal high stemness index breast cancer cohorts

The paper investigates glycosylation's role in breast cancer stemness, finding distinct glycosylation pathways in Basal and Luminal B subtypes that correlate with different clinical outcomes.

nsGSLs on tumors impair anti-tumor immune responses by OT-I T cells in vitro and support tumor growth in vivo

This study shows that tumor-expressed nsGSLs impair OT-I T cell activation in vitro and promote tumor growth in vivo by evading immune responses.

The N-glycosylation defect in Lec5 and Lec9 CHO cells is caused by absence of the DHRSX gene

This study identifies DHRSX gene absence as the cause of dolichol synthesis defect in Lec5 and Lec9 CHO cells, correcting N-glycosylation defects through DHRSX complementation.

Scalable Extraction of Airway Mucins from Porcine Trachea

This paper describes a method to isolate airway mucins from pig trachea at a large scale for pulmonary disease research, showing higher mucin content and microviscosity compared to gastric mucins and human mucus.

The wondrous and worrying diversity of the N-glycans of Chlorella food supplements

The paper analyzes N-glycan diversity in Chlorella supplements, finding mismatches between product labels and glycan patterns, highlighting concerns about product authenticity.

Neuronal loss of Galnt2 in Mice Impairs O-glycosylation and Recapitulates Neurological Features of GALNT2-CDG

This study shows that loss of Galnt2 in mice leads to neurological symptoms similar to GALNT2-CDG and identifies O-glycosylation changes in the brain.

Cell and tissue-specific glycosylation pathways informed by single-cell transcriptomics

The paper analyzes single-cell glycogene expression in human tissues to identify cell- and tissue-specific glycosylation pathways and transcription factor regulation.

Evolutionary rate covariation is pervasive between glycosylation pathways and points to potential disease modifiers

The paper identifies potential genetic modifiers of Congenital Disorders of Glycosylation (CDG) using evolutionary rate covariation analysis across glycosylation pathways and other biological processes.

Glycoengineering with neuraminic acid analogs to label lipooligosaccharides and detect native sialyltransferase activity in Gram-negative bacteria

The paper develops a method to label lipooligosaccharides in Gram-negative bacteria using neuraminic acid analogs and detects sialyltransferase activity in live bacteria.

O-glycosylation contributes to mammalian glycoRNA biogenesis

This study demonstrates that O-glycosylation contributes to sialoglycoRNA biogenesis and shows increased sialylation in ulcerative colitis-derived organoids.

Navigating the Maze of Mass Spectra: A Machine-Learning Guide to Identifying Diagnostic Ions in O-Glycan Analysis

This paper develops a machine learning workflow to identify generalizable diagnostic ions for O-glycan isomer differentiation using a curated dataset of 237,000 spectra.

GRable version 1.0: A software tool for site-specific glycoform analysis with improved MS1-based glycopeptide detection with parallel clustering and confidence evaluation with MS2 information

Remodelling of the glycome of B-cell precursor acute lymphoblastic leukemia cells developing drug-tolerance

The paper identifies glycotraits in drug-tolerant BCP-ALL cells that correlate with chemotherapy resistance.

The modified RNA base acp3U is an attachment site for N-glycans in glycoRNA

The paper identifies acp3U as the site where N-glycans attach to RNA in glycoRNA using rPAL and SWATH-MS.

Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in Escherichia coli

The paper identifies a single-subunit oligosaccharyltransferase from Desulfovibrio marinus that enables glycosylation of full-length IgG antibodies in E. coli.

Deep Learning Prediction of Glycopeptide Tandem Mass Spectra Powers Glycoproteomics

DeepGP predicts glycopeptide MS/MS spectra and retention time using a hybrid deep learning framework, improving glycoproteomics analysis by distinguishing similar glycoforms and increasing detection sensitivity.

Adipose microenvironment promotes hypersialylation of ovarian cancer cells

This study shows that the adipose microenvironment increases sialylation in ovarian cancer cells, promoting tumor growth in immune-dependent settings.

N-Glycosylation of MRS2 balances aerobic and anaerobic energy production by reducing rapid mitochondrial Mg2+ influx in conditions of high glucose or impaired respiratory chain function

This study shows that N-glycosylation of MRS2, a mitochondrial Mg2+ transporter, regulates Mg2+ influx and balances energy production under high glucose or impaired respiration.

GlyTrait Brings Insights into Functional Glycosylation

GlyTrait is a Python framework that calculates and interprets functional glycan traits from N-glycome data, improving interpretability and disease association analysis.

Predicting glycan structure from tandem mass spectrometry via deep learning

The paper introduces CandyCrunch, a deep learning model that predicts glycan structures from tandem mass spectrometry data with high accuracy, enabling high-throughput glycomics.

Aberrant N-glycosylation is a therapeutic target in carriers of a common and highly pleiotropic mutation in the manganese transporter ZIP8

This study shows that a ZIP8 mutation causes aberrant N-glycosylation in Crohn's disease, which can be treated with N-acetylglucosamine supplementation.