Antibiotic Resistance in Bacteria

Plasmidome, resistome, and virulence-associated genes characterization of Acinetobacter johnsonii in NASA cleanrooms and a clinical setting.

This study characterizes the plasmidome, resistome, and virulence genes of Acinetobacter johnsonii from NASA cleanrooms and a clinical setting, revealing adaptability traits and antimicrobial resistance.

Single point mutations in global regulatory genes restore cephalosporin resistance in a low-MIC Enterococcus faecium natural isolate

Resistome, Virulome, Mobilome, And Biosynthetic Gene Clusters Adaptations of Acinetobacter Baumannii Mexican Strains Across the Pre- and of the COVID-19 Period: Insights from Whole-Genome Sequencing

This study compares the genomic adaptations of Acinetobacter baumannii strains in Mexico before and during the COVID-19 period using whole-genome sequencing, revealing increased resistance genes, virulence factors, and mobilome elements.

Occurrence of Carbapenem-resistant Enterobacterales in swine wastewater in Shandong Province, China

This study identifies carbapenemase-producing E. coli in swine wastewater in Shandong, China, highlighting their multidrug resistance and genetic diversity.

NCY-I beta-lactamase activity correlates with antimicrobial susceptibility of a clinical strain of Nocardia cyriacigeorgica

The paper investigates the beta-lactam susceptibility of a clinical Nocardia cyriacigeorgica strain and links it to the activity of its NCY-I beta-lactamase enzyme.

Cost and benefits of gene amplification-mediated antibiotic resistance

This study shows that gene amplification increases antibiotic resistance but also imposes growth costs and increases susceptibility to high antibiotic concentrations.

TraN variants mediate conjugation species specificity of IncA/C, IncH and Acinetobacter baumannii plasmids

Emergence of a novel hypervirulent extensively drug-resistant ST383 Klebsiella pneumoniae lineage carrying ICEKp5 in Lebanon

This study identifies a novel hypervirulent extensively drug-resistant ST383 K. pneumoniae lineage in Lebanon carrying ICEKp5 and multiple resistance genes.

Within-Patient Evolution of Pseudomonas aeruginosa Populations During Antimicrobial Treatment

This study tracks genomic changes in Pseudomonas aeruginosa during antimicrobial treatment using single-isolate and metagenomic sequencing, revealing resistance mutations and population diversity.

In vitro activity of combination formulations of the novel metallo-β-lactamase (MBL) inhibitor APC148 with comparator treatments against 176 MBL-containing Enterobacterales isolates from the SENTRY Antimicrobial Surveillance Program (2019-2022)

This study evaluates the in vitro activity of APC148 combined with carbapenems against MBL-producing Enterobacterales isolates, showing high susceptibility rates.

Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation

This study shows plasmids contribute to antimicrobial resistance by promoting gene inactivation via insertion sequences in Klebsiella pneumoniae.

A novel mechanism of ceftolozane-tazobactam resistance in Pseudomonas aeruginosa mediated by L2 β-lactamase

The paper identifies a novel mechanism of ceftolozane-tazobactam resistance in Pseudomonas aeruginosa mediated by an L2 β-lactamase gene, independent of its canonical regulator.

Rapid and Interpretable AMR Diagnostics via Genomics and Cell Painting using Differential Geometry-based Directed-Simplicial Neural Networks on Multimodal Data

This paper develops a multimodal AI model for rapid AMR prediction using genomic and cellular data, identifying key genomic motifs and phenotypic features associated with antimicrobial resistance.

Active destabilization of the integron synaptic complex reduces bacterial adaptation to antibiotics

The paper shows that destabilizing the integron synaptic complex with peptides reduces bacterial adaptation to antibiotics without antimicrobial activity.

Forecasting multi-trait resistance evolution under antibiotic stress

This study uses a mathematical framework to simulate in silico evolution of Pseudomonas aeruginosa under antibiotic stress, revealing mutational patterns and resistance mechanisms.

AMR-GNN: A multi-representation graph neural network framework to enable genomic antimicrobial resistance prediction

AMR-GNN predicts antimicrobial resistance using a graph neural network framework that integrates multiple genomic representations from whole-genome sequencing data.

Fitness effects of antimicrobial resistance genes in changing environments

This study measures the fitness effects of 136 antimicrobial resistance genes in changing environments and finds that oxygen availability modulates their cost.

In-vitro and in-vivo efficacy of a novel broad spectrum β-lactamase inhibitor APC24-7 against Enterobacterales

This study evaluates APC24-7, a novel β-lactamase inhibitor, showing it restores meropenem activity against CRE in vitro and in vivo.

In vivo de-amplification of a multi-resistance pseudo-compound transposon in Escherichia coli

This study tracks the in vivo evolution of an antibiotic-resistant E. coli strain in an infant gut, showing a reduction in IS26-mediated resistance gene arrays without fitness or susceptibility changes.

Analysing antimicrobial resistance mobility patterns using a diverse dataset of over 8000 bacterial species

The paper analyzes antimicrobial resistance gene mobility across 8000 bacterial species, finding that certain resistance mechanisms are more associated with mobile elements than others.

Molecular characterisation of a Klebsiella pneumoniae neonatal sepsis outbreak in a rural Gambian hospital: a retrospective genomic epidemiology investigation

This paper uses genomic epidemiology to characterize a K. pneumoniae sepsis outbreak in The Gambia, identifying the outbreak strain and its environmental sources.

Magnesium depletion unleashes two unusual modes of colistin resistancewith different fitness costs

This study identifies two distinct mechanisms of colistin resistance in P. aeruginosa driven by magnesium depletion, each with different fitness costs.

Bridging disciplines towards wastewater-based surveillance of antimicrobial resistance: frequency, local dynamics, and genomic characteristics of carbapenemase-producing Klebsiella pneumoniae

This paper develops a workflow for detecting carbapenemase-producing Klebsiella pneumoniae in wastewater using wet-lab methods and genomics, finding high prevalence and specific genetic markers.

Allosteric Inhibition of NDM-1 by Thanatin Preserves the Di-Zinc Center While Restricting Dynamics

This paper reveals that Thanatin inhibits NDM-1 by preserving its zinc center while restricting dynamics, contrasting with zinc-displacement models and showing reduced bacterial viability.

Overexpression of Escherichia coli yaiX Confers Multidrug Resistance and Enhances Virulence in the Silkworm Infection Model

This study identifies YaiX overexpression in E. coli as a factor conferring multidrug resistance and enhanced virulence in silkworms.

Plant associated bacteria are a rich reservoir for multidrug efflux pumps

The paper analyzes RND efflux pump genes in plant-associated bacteria, finding increased diversity and horizontal gene transfer contributions to adaptation.

Metagenomic analysis of antimicrobial resistance, virulence, and mobile genetic elements in the gut microbiota of Caprinae species

This study analyzes the gut microbiota of Caprinae species to identify antimicrobial resistance genes, virulence factors, and mobile genetic elements, revealing potential zoonotic transmission pathways.

A One Health study of Klebsiella pneumoniae species complex plasmids shows a highly diverse and ecologically adaptable plasmidome

This study characterizes the diversity and niche associations of plasmids in Klebsiella pneumoniae species complex isolates from human, animal, and marine sources, revealing distinct patterns of antimicrobial resistance and virulence gene distribution.

Structural and Computational Analysis of Pseudomonas Aeruginosa DNA Gyrase Reveals Molecular Characteristics That May Contribute to Ciprofloxacin Resistance

The paper analyzes the molecular mechanisms of ciprofloxacin resistance in Pseudomonas aeruginosa by solving the gyrase structure and performing MD simulations of wild-type and mutant systems.

Whole-genome sequencing reveals inter-household networks of gut-colonising ESBL-producing Escherichia coli in two rural Malawian districts

The paper uses whole-genome sequencing to study ESBL-Ec transmission networks in rural Malawi, finding high colonization rates and multi-household spread.