Heat shock proteins research

RiboTag RNA Sequencing Identifies Local Translation of HSP70 In Astrocyte Endfeet After Cerebral Ischemia

The paper identifies HSP70 local translation in astrocyte endfeet after cerebral ischemia using RiboTag RNA sequencing and finds it correlates with proteostasis and ubiquitination changes.

Structural insights into GrpEL1-mediated nucleotide and substrate release of human mitochondrial Hsp70

The paper determines the structure of human mortalin-GrpEL1 complexes using cryoEM and identifies mechanisms of nucleotide and substrate release in the Hsp70 system.

Localized synthesis of molecular chaperones sustains neuronal proteostasis

The paper shows that neuronal stress response involves localized synthesis of HSPA8 mRNA in dendrites to maintain proteostasis and prevent neurodegeneration.

The Novel Chaperone Activity of p47 Modulates Force-Mediated Retrotranslocation from the Endoplasmic Reticulum to the Cytosol

This study investigates p47's role in retrotranslocation by enhancing talin's mechanical stability and generating pulling forces to facilitate ER-to-cytosol protein export.

Arabidopsis EID1 E3 ubiquitin ligase regulates acquired thermotolerance by modulating HSBP translocation

This study identifies EID1 as an E3 ubiquitin ligase that regulates acquired thermotolerance by modulating HSBP translocation during heat stress.

Mechanistic Insights into Periplasmic Chaperones as Regulators of Protein Folding and Translocation in Gram-Negative Bacteria

This study investigates the roles of periplasmic chaperones in Gram-negative bacteria, showing some act as foldases and others as holdases during protein translocation.

Acetylation of the yeast Hsp40 chaperone protein Ydj1 fine-tunes proteostasis and translational fidelity

This study identifies acetylation sites on yeast Ydj1 that affect its interactions with chaperones and client proteins, impacting proteostasis and translational fidelity.

HSP70 is upregulated after heat but not freezing stress in the freeze-tolerant cricket Gryllus veletis

This study examines HSP70 expression in a freeze-tolerant cricket under heat and freezing stress, finding that HSP70 is upregulated by heat but not freezing.

ClpS directs degradation of primary N-end rule substrates in Mycolicibacterium smegmatis

This study identifies ClpS as a key regulator of primary N-end rule proteolysis in Mycolicibacterium smegmatis, showing it binds canonical N-degrons and enhances substrate specificity for ClpC1P1P2 protease.

Design principles to tailor Hsp104 therapeutics

This paper identifies design principles for modifying Hsp104 to enhance its therapeutic potential by tuning its collaboration with Hsp70 and Hsp40.

Dynamic regulation of RNA Polymerase III transcription in mouse embryonic stem cells during heat shock stress

The paper examines how RNA Polymerase III transcription changes in mouse embryonic stem cells during heat shock, showing initial downregulation followed by upregulation, with HSF1 involvement in the latter.

A pharmacological chaperone stabilizer rescues the expression of the vast majority of pathogenic variants in a G protein-coupled receptor

The paper tests a pharmacological chaperone's ability to rescue expression of destabilizing variants in the V2R gene, finding it effective for most variants.

HSP90 buffers deleterious genetic variations in BRCA1

This study shows HSP90 buffers BRCA1 mutations, reducing their clinical severity and contributing to PARP inhibitor resistance in cancer cells.

Calmodulin acts as a chaperone during co-translational folding of the Kv7.2 channel Calcium Responsive Domain.

This study shows calmodulin acts as a chaperone during co-translational folding of the Kv7.2 channel's calcium responsive domain by stabilizing metastable hairpins.

Allosteric modulation of the Lon protease by effector binding and local charges

This study shows that ssDNA binding and charge changes at DNA binding sites regulate Lon protease activity through allosteric effects and oligomerization.

Proteostasis Associated Variants in HSPB5 Destabilize the αB-crystallin Domain

This study identifies three deleterious SNPs in HSPB5 that disrupt the αB-crystallin domain's structure and function, contributing to proteotoxicity-related diseases.

A proteolytic AAA+ machine poised to unfold a protein substrate

The paper presents a cryo-EM structure of a ClpXP-substrate complex, revealing an intermediate state in protein unfolding by the AAA+ proteolytic machine.

Preferential binding of ADP-bound mitochondrial HSP70 to the nucleotide exchange factor GRPEL1 over GRPEL2

This study shows ADP-bound mtHSP70 binds preferentially to GRPEL1 over GRPEL2, with structural insights into their functional differences.

Dynamic Insights into Hsp90 Inhibitor Binding: Uncovering Intermediate States and Conformational Plasticity

This study uses MD simulations and machine learning to reveal a two-stage binding mechanism of Hsp90 inhibitor Geldanamycin, showing stabilization of the loop-out conformation and involvement of both conformational selection and induced fit.

Stabilization of HSA32, an aggregation-prone protein, by the protein disaggregase HSP101 plays a critical role in maintaining acquired thermotolerance in Arabidopsis

This study shows that HSP101 stabilizes HSA32, an aggregation-prone protein, which is essential for maintaining heat acclimation memory in Arabidopsis.

The ATP-dependent protease ClpYQ degrades cell division proteins DivIVA and Mbl in Bacillus subtilis

The paper identifies DivIVA and Mbl as substrates of the ClpYQ protease in Bacillus subtilis, showing their degradation in vitro and in vivo.

Short heat shock factor A2 confers heat sensitivity in Arabidopsis: Insights into heat resistance and growth balance

This study identifies S-HsfA2 in Arabidopsis as a factor that prevents hyperactivation of the heat stress response, balancing heat resistance and growth.

Age-dependent Changes in a Chaperone Complex in the Mouse Heart

This study shows that in aging mouse hearts, Unc45b and Hsp70 levels decline at the protein level, while Hsp90 remains stable, potentially contributing to age-related cardiomyopathies.

Fitness defects due to cytosolic protein misfolding in S. cerevisiae can be alleviated by decreasing mitochondrial protein import capacity

The paper identifies that reducing mitochondrial protein import capacity alleviates cytosolic protein misfolding fitness defects in yeast by enhancing proteostasis.

A dynamic redox switch turns TRC40 into a chaperone protecting human cells against ATP-depleting, oxidative stress

This study shows TRC40 switches from an ATP-dependent targeting factor to an ATP-independent chaperone under oxidative stress, preventing protein aggregation and supporting proteostasis.

Loss of αBa-crystallin, but not αA-crystallin, increases age-related cataract in the zebrafish lens

This study shows that loss of zebrafish Ba-crystallin (cryaba) increases age-related cataract, while loss of A-crystallin (cryaa) does not, highlighting an ontogenetic shift in crystallin function during lens development.

Modification of regulatory tyrosines biases human Hsp90α for interaction with cochaperones and clients

This study investigates how phosphorylation of tyrosine residues in human Hsp90 affects its interactions with cochaperones and clients using yeast and cell-based assays.

Structural and mechanistic studies on human LONP1 redefine the hand-over-hand translocation mechanism

This study redefines the hand-over-hand translocation mechanism of LONP1 using cryo-EM structures and biochemical assays, showing substrate binding regulates activity and ADP-bound ATPases can support translocation.

Inhibition of the HSP90 homodimerization and HSP90-HIF1α interactions by employing small molecules at C-terminal ATP binding site of HSP90

This study identifies indole-containing compounds that disrupt HSP90-HIF1α interactions by binding to the C-terminal ATP site, showing potential for repurposing tryptamine derivatives to treat hypoxic tumors.

Driving proteomic imbalance in malignancy provokes proteomic catastrophe and confers tumor suppression

This study shows that loss of HSF1 in MPNST cells causes proteomic imbalance and tumor suppression by inducing proteomic catastrophe through amyloidogenesis and mTORC1 inhibition.