flow cytometry

Minimal impact of ivermectin on immune response and transcriptional profiles in naïve adults with mild COVID-19

This study evaluates the immunomodulatory effects of ivermectin in mild COVID-19 patients and finds no significant immune response changes, consistent with clinical trial results.

Triple Negative Breast Cancer Cells Acquire Lymphocyte Proteins and Genomic DNA During Trogocytosis with T Cells

This study shows TNBC cells acquire CD45 proteins and T cell genomic DNA through trogocytosis, altering their gene expression and expressing CD45 on and inside the cells.

Single-cell profiling of CAR-T CD19 cell phenotypes and immune system dynamics in pediatric BCP-ALL

This study characterizes CAR+ T-cell subsets and immune system dynamics in pediatric BCP-ALL patients treated with tisagenlecleucel, finding correlations between immune cell changes and cytokine release syndrome.

Longitudinal cellular and humoral immune responses following Covid-19 BNT162b2-mRNA-based booster vaccination of craft and manual workers in Qatar.

This study examines immune responses in craft and manual workers after a BNT162b2 booster, showing enhanced Th1 and cytotoxic T cell responses, and durable humoral immunity, with prior infection boosting B cell counts.

Bacterial vaginosis-driven changes in cervicovaginal immunity that expand the immunological hypothesis for increased HIV susceptibility

This study investigates how bacterial vaginosis alters cervicovaginal immunity, identifying immune cell changes that may contribute to increased HIV susceptibility.

Comprehensive longitudinal profiling of SARS-CoV-2-specific CD8+ T-cells reveal strong functional impairment and recognition bias as markers for disease severity

This study identifies antigen-specific CD8+ T-cell responses linked to disease severity in SARS-CoV-2 infection, showing functional impairment and recognition bias.

Lactobacillus acidophilus ameliorates inflammatory bone loss under post-menopausal osteoporotic conditions via preventing the pathogenic conversion of gut resident pTregs into Th17 cells

CD45-mediated apoptosis and IL2R downregulation by serine proteases from diarrheagenic bacteria

This paper shows that serine proteases from diarrheagenic bacteria cleave CD45 and induce T-cell apoptosis, mimicking IL-2 deprivation and contributing to bacterial virulence.

ApoE ε4-dependent alteration of CXCR3+CD127+ CD4+ T cells is associated with elevated plasma neurofilament light chain in Alzheimer's disease

The paper finds that ApoE ε4-dependent changes in CXCR3+CD127+ CD4+ T cells in peripheral blood correlate with elevated neurofilament light chain in Alzheimer's disease.

BASTA, a simple whole blood assay for measuring beta-cell antigen specific CD4+ T-cell responses in type 1 diabetes

Novel cytometry-based characterization of lysosomal storage disease affected patient's cells

This paper characterizes lysosomal storage disease using imaging and cytometry to assess lipid homeostasis in patient cells and validates findings in alternative models.

An early functional adaptive NK cell signature drives optimal CD8+ T-cell activation and predicts sustained HIV-1 viral control.

The paper identifies an early expansion of adaptive NK cells during acute HIV-1 infection that correlates with long-term viral control.

Inferring cell dynamics in stress-induced neuroblastoma cell cultures

The paper quantifies cell dynamics and interactions in neuroblastoma cell cultures under stress, finding that cell-to-cell interactions drive phenotypic adaptation, which accelerates with lower nutrient levels.

LSD1 inhibition corrects dysregulated MHC-I and dendritic cells activation through IFNγ-CXCL9-CXCR3 axis to promote antitumor immunity in HNSCC

This study shows LSD1 inhibition enhances antitumor immunity in HNSCC by upregulating MHC-I and activating the IFNγ-CXCL9-CXCR3 axis through dendritic cells and CD8+ T cells.

Immune-profiling of T helper 1 (Th1), Th2 and Th17 signatures in murine splenocytes by targeting intracellular cytokines

The paper develops a 17-parameter spectral flow cytometry panel to profile Th1, Th2, and Th17 cytokine signatures in murine splenocytes under steady-state and stimulated conditions.

Immunometabolic analysis of primary murine Group 2 Innate Lymphoid Cells: a robust step-by-step approach

This paper provides a step-by-step framework to analyze immunometabolic states of primary murine ILC2s and link them to phenotypes and functionality.

Predictive Modeling of acute Graft-versus-Host-Disease using Machine Learning on Immune Cell and Cytokine Profiles at Engraftment

This study uses machine learning to predict acute Graft-versus-Host-Disease (aGvHD) based on immune cell and cytokine profiles at engraftment, finding high accuracy with cytokine and T-cell data.

Functional Dichotomy of Developmental Foxp3+ Treg Cell Subsets in the Visceral Adipose Tissue of Lean and Obese Mice

This study investigates the functional differences between thymic and peripheral Treg cells in visceral adipose tissue, showing that tTreg instability contributes to inflammation and obesity while pTreg cells protect against metabolic disorders.

In vivo antibody labeling route and fluorophore dictate labeling efficiency, sensitivity, and longevity

This study shows that changing antibody administration route and fluorophore improves leukocyte labeling efficiency, sensitivity, and duration for tracking migration.

A hepatocyte-specific cytochrome c oxidase deficiency in mice leads to a lymphopenia owing to deficiencies in bone marrow progenitors

This study shows that hepatocyte-specific cytochrome c oxidase deficiency in mice causes lymphopenia due to bone marrow progenitor defects and early autoimmunity.

Cultured endothelial cells present organ-specific RTK distributions: advancing receptor measurement and data standardization via quantitative flow cytometry

The paper quantifies plasma membrane and whole-cell RTK expression in cultured human endothelial cells from different organs, finding organ-specific distributions that may not fully reflect angiogenic capacities in vitro.

Pediatric cerebrospinal fluid immune profiling distinguishes pediatric-onset multiple sclerosis from other pediatric-onset acute neurological disorders

This study identifies immune profiles in pediatric cerebrospinal fluid to differentiate pediatric-onset multiple sclerosis from other inflammatory neurological disorders.

Single-cell mass cytometry maps the evolution of immune cell signatures predictive of acute graft-versus-host disease

The paper uses mass cytometry to identify immune cell signatures predictive of acute graft-versus-host disease (aGvHD) and shows how these signatures evolve over time.

High-dimensional spectral flow cytometry of activation and phagocytosis by peripheral human polymorphonuclear leukocytes

The paper introduces a 22-color spectral flow cytometry panel to profile human PMNs for activation, degranulation, phagocytosis, and other responses using phorbol ester and bacterial challenges.

Comprehensive analysis of myeloid reporter mice

This study presents lineage tracing results from 18 myeloid-specific fate-mapping models in mouse peripheral blood and synovial tissue, validating synovial macrophage identification through flow cytometry and CITE-seq.

Natural Killer Cell Receptor Signaling and Activation Depend on Cell Cycle Stages

This study shows that NK cell receptor signaling and activation vary with cell cycle stages, with some proteins showing non-monotonic abundance changes.

Stochastic Regression and Peak Delineation with Flow Cytometry Data

The paper introduces a method to separate signal and noise in flow cytometry data to improve genome copy number estimation in E. coli.

Isolation of individual natural killer cells from deep, high-aspect ratio microwell arrays - an evaluative study

This study evaluates a microwell system for isolating individual NK cells, demonstrating its viability and potential for functional screening but noting its limited throughput.

Immunological characterization of peritoneal exudate cells in liver cirrhosis patients

This study characterizes peritoneal exudate cells in liver cirrhosis patients using single-cell and bulk RNA sequencing, revealing shifts in immune cell populations and gene expression with disease severity.

Distinct metabolic profiles of circulating plasmacytoid dendritic cells in systemic sclerosis patients stratified by clinical phenotypes

The paper examines metabolic profiles of circulating plasmacytoid dendritic cells (pDCs) in systemic sclerosis (SSc) patients, finding differences based on clinical phenotypes.