flow cytometry

Lysyl oxidase promotes actin-dependent neutrophil activation and cytotoxicity in diabetes: Implications for diabetic retinopathy

This study shows LOX promotes actin-dependent neutrophil activation and cytotoxicity in diabetes, contributing to diabetic retinopathy through F-actin remodeling and granule clustering.

Lymphocytes and monocytes undergo swift suppression of IL-10R, IL-6R, and IL-2Rβγ signaling under high concentrations of different cytokines

The paper shows that lymphocytes and monocytes suppress IL-10R, IL-6R, and IL-2Rβγ signaling under high cytokine concentrations, mediated by cytokines that do not induce their own pSTATs.

CD24 regulates the formation of ectosomes in B lymphocytes

This study identifies a novel pathway involving CD24, aSMase, PI3K/mTORC2/ROCK/actin that regulates bioactive ectosome formation in B lymphocytes.

Transcriptomic alterations including p53 pathway dysregulation prime DNMT3A mutant cells for transformation

This study shows DNMT3A mutations reduce DNA methylation and disrupt p53 pathway, increasing self-renewal and cancer risk in hematopoietic cells.

Using artificial intelligence-based software for an unbiased discrimination of immune cell subtypes in the fracture hematoma and bone marrow of non-osteoporotic and osteoporotic mice

The paper uses AI-based clustering to identify immune cell subtypes in fracture hematoma and bone marrow of mice, revealing differences between osteoporotic and non-osteoporotic groups.

A Characterization of the Immune Cells in Immunocompetent and Immunodeficient Mice with Orthotopic Brain Tumors.

This study characterizes immune cell composition in immunocompetent and immunodeficient mice with orthotopic brain tumors, finding significant differences in immune cell frequencies across tissues and after irradiation.

Angiogenic and Immune Predictors of Neoadjuvant Axitinib Response in Renal Cell Carcinoma with Venous Tumour Thrombus

The paper assesses neoadjuvant axitinib response in RCC with VTT using multiparametric analysis and identifies angiogenic and immune predictors of treatment response.

Memory T and B cells with recognition of avian influenza hemagglutinins are poorly responsive to existing seasonal influenza vaccines

The paper quantifies pre-existing human B cell responses to avian influenza H5 and H7 hemagglutinins and shows limited boosting by seasonal vaccines.

Dexamethasone-Induced p57-Mediated Quiescence Drives Chemotherapy Resistance in Sonic Hedgehog Medulloblastoma

Low Dose Methotrexate has Divergent Effects on Cycling and Resting Human Hematopoietic Stem and Progenitor Cells

This study shows low dose methotrexate (LD-MTX) differentially affects cycling and resting human hematopoietic stem and progenitor cells (HSPC), reducing proliferation while preserving clonogenicity in non-cycling cells.

HIGH THROUGHPUT QUANTITATION OF HUMAN NEUTROPHIL RECRUITMENT AND FUNCTIONAL RESPONSES IN AN AIR-BLOOD BARRIER ARRAY

The paper develops a high-throughput assay to study neutrophil recruitment and function in an air-blood barrier array, showing improved throughput and sample efficiency compared to existing methods.

Deconvoluting TCR-dependent & -independent activation is vital for reliable Ag-specific CD4+ T cell characterization by AIM assay

The paper shows that AIM assays overestimate Ag-specific CD4+ T cells due to TCR-independent activation and introduces a modified assay using CXCR4-CD137+ cells for accurate characterization.

Apparent cooperativity between human CMV virions introduces errors in conventional methods of calculating multiplicity of infection

The paper investigates apparent cooperativity between human CMV virions and shows it affects multiplicity of infection calculations.

Influenza virus infection in the lungs leads to pancytopenia and defective immune cell differentiation program in the thymus and bone marrow

This study shows that influenza virus infection causes reduced immune cell counts and impaired differentiation in the thymus and bone marrow, linked to systemic inflammation and oxidative stress.

Fluorescence-barcoded cell lines stably expressing membrane-anchored influenza neuraminidases

The paper develops fluorescence-barcoded cell lines expressing membrane-anchored influenza neuraminidases to study antibody responses and vaccine efficacy.

Whole-Proteome Screening and Multi-Modal Profiling of Antigen-Specific CD4+ T Cells at Single-Cell Resolution

The paper develops a high-throughput platform to profile antigen-specific CD4+ T cells at single-cell resolution, identifying immunogenic features and functional heterogeneity in viral and cancer contexts.

Alpha-1 Antitrypsin Overexpressing Mesenchymal Stem/Stromal Cells Reverses Type 1 Diabetes via Promoting Treg Function and CD8+ T cell exhaustion

This study shows that AAT-overexpressing MSCs reverse T1D in NOD mice by enhancing Treg function and inducing CD8+ T cell exhaustion.

Neutrophils respond with pathogen-specific defenses during bacterial pneumonia

This study identifies pathogen-specific neutrophil surface protein phenotypes in bacterial pneumonia, revealing tissue- and infection-type dependent immune responses.

Meningococcal vaccine Bexsero elicits a robust cellular immune response that targets but is not consistently protective against Neisseria gonorrhoeae during murine vaginal infection

This study analyzes the immune response to Bexsero vaccine in mice, showing robust humoral and cellular responses but inconsistent protection against Neisseria gonorrhoeae.

An Optimized LIVE/DEAD Assay Using Flow Cytometry to Quantify Post-Stress and Antifungal-Treatment Survival in Diverse Yeasts

The paper optimizes a LIVE/DEAD assay using flow cytometry to quantify post-stress and antifungal-treatment survival in yeasts, identifying an intermediate cell population with enhanced SYTO 9 uptake.

Glycosylated IgG antibodies contribute to the recovery of haemorrhagic fever with renal syndrome patients

This study investigates IgG glycosylation patterns in HFRS patients, finding increased fucosylated IgG and decreased bisecting N-acetylglucosamine during recovery, with correlations between glycosylation and B cell subsets.

Protection against N. gonorrhoeae induced by OMV-based Meningococcal Vaccines are associated with cross-species directed humoral and cellular immune responses

This study investigates cross-species immune responses induced by meningococcal OMV vaccines and their association with protection against N. gonorrhoeae.

Membrane vesicles in freshwater ecosystems: Quantification by flow cytometry and interferometric microscopy

This study quantifies membrane vesicles in freshwater ecosystems using flow cytometry and interferometric microscopy, finding agreement in nanoparticle abundance and suggesting associations with virus particles or dense compounds.

Polysialylated NCAM distinguishes differentiated myoblasts from proliferating myoblasts prior to fusion

The paper identifies polysialylated NCAM as a marker distinguishing differentiated from proliferating myoblasts before fusion.

FLASH radiotherapy spares lymphocytes in tumor-draining lymph nodes and increases infiltration of immune cells in tumors

This study shows FLASH radiotherapy preserves lymphocytes and increases immune cell infiltration in tumors, potentially improving antitumor immune responses.

PLCg2 Controls Neutrophil Sensitivity through Calcium Oscillation and Gates Chemoattractant Concentration Range for Chemotaxis

This study shows that PLCγ2 controls neutrophil sensitivity via calcium oscillations and regulates chemoattractant concentration range for chemotaxis.

Bacterial colony biopsies: spatial discrimination of heterogeneous cell types by cytometric fingerprinting

This study introduces a biopsy technique combined with cytometric fingerprinting to analyze spatial heterogeneity in bacterial colonies, revealing distinct physiological states across different locations.

Diverse priming outcomes under conditions of very rare precursor B cells

The paper investigates how vaccine antigen delivery and adjuvant selection affect the priming of rare HIV broadly neutralizing antibody-precursor B cells in non-human primates, finding that specific dosing and adjuvant combinations enhance their detection in germinal centers.

Rare Subset of T Cells Form Heterotypic Clusters with Circulating Tumor Cells to Foster Cancer Metastasis

The paper identifies a rare subset of CD4 and CD8 double positive T cells that form clusters with circulating tumor cells, promoting cancer metastasis through immune suppression and integrin-mediated interactions.

Two distinct durable human class-switched memory B cell populations are induced by vaccination and infection