Reproductive Biology and Fertility

A sperm-oocyte protein partnership required for egg activation in Caenorhabditis elegans

This study identifies a sperm-oocyte protein complex, SPE-11 and OOPS-1, essential for meiotic completion, polyspermy block, and eggshell formation in C. elegans.

Biological characteristics of rabbit KLF12 and its regulation of proliferation and apoptosis of ovarian granulosa cells

This study investigates the role of KLF12 in regulating proliferation and apoptosis of rabbit ovarian granulosa cells, revealing its impact on follicular development and hormone secretion.

Disrupted MOS signaling alters meiotic cell cycle regulation and the egg transcriptome

This study shows that MOS signaling is essential for meiotic cell cycle regulation and maintaining the egg transcriptome required for developmental competence.

Exploring the Dynamics of Follicle Development and Hormone Synthesis: The Role of Oxygen Tension in Rhesus Macaque Follicle Culture

This study investigates the effects of reduced oxygen tensions on follicle survival, growth, and hormone production in cultured rhesus macaque follicles, finding that lower oxygen levels improve outcomes.

Fertility recovery after combined adriamycin, bleomycin, vinblastine and dacarbazine chemotherapy in mice

This study shows that ABVD chemotherapy in mice leads to temporary ovarian dysfunction but allows recovery of fertility and ovarian reserve.

Aging-associated vacuolation of multi-ciliated cells in the distal mouse oviduct reflects unique cell identity and luminal microenvironment

This study identifies age-related vacuolation in multi-ciliated cells of the distal mouse oviduct, linked to reduced blood flow and unique cellular identity in the infundibulum and ampulla.

Three-dimensional culture in a bioengineered matrix and somatic cell complementation to improve growth and survival of bovine preantral follicles

This study shows that 3D PEG hydrogels improve bovine preantral follicle growth compared to 2D culture, and bESCs can differentiate into gonadal somatic cell precursors.

Generation and Assessment of High-Quality Fat-Tailed Dunnart Oocytes Following Superovulation in Prepubertal Animals

This study develops a hormonal regimen to induce ovulation and collect mature oocytes from prepubertal fat-tailed dunnarts, achieving high success rates in oocyte generation and embryo development.

Single cell analysis of ovarian immune cells during homeostasis and hormonal flux reveals dynamic changes in NK and B cell populations in the periovulatory phase

The paper uses single-cell RNA sequencing and flow cytometry to analyze ovarian immune cells in mice under high and low estradiol states, finding dynamic changes in NK and B cell populations during the periovulatory phase.

Cell-penetrating peptide-mediated mouse oocyte activation

This study uses a cell-penetrating peptide fused to Emi2 to activate mouse oocytes, showing it can induce pronuclear formation and blastocyst development.

Nanosecond pulsed electric field precisely manipulates cytoplasmic Ca2+ oscillations during oocyte activation

This study uses nanosecond pulsed electric fields to manipulate Ca2+ signaling in oocytes, improving activation efficiency and developmental potential compared to traditional methods.

DILP8 serves as a mature follicle sensor to prevent excessive accumulation of maturefollicles in Drosophila ovaries and oocyte aging.

This study identifies DILP8 as a sensor for mature follicle accumulation in Drosophila ovaries, preventing excessive follicles and maintaining oocyte quality through the Lgr3 receptor.

Oxygen level alters energy metabolism in bovine preimplantation embryos

This study shows that hypoxia (6% O2) improves blastocyst formation in bovine embryos compared to normoxia (20% O2) and ultrahypoxia (2% O2), with normoxia altering energy metabolism and reducing blastocyst rates.

Long-term iododeoxyuridine labelling analysis finds no evidence of ovarian germline stem cell activity in adult or ageing mice

This study uses IdU labeling to investigate ovarian germline stem cell activity in mice and finds no evidence of such activity in adult or aging mice.

Computational Modeling of the Biphasic Depletion of Ovarian Follicle Reserve and the Chemical Effects on Ovarian Aging

This paper develops a mechanistic model of ovarian follicle depletion and evaluates how chemical exposures affect the biphasic depletion pattern and menopausal timing.

Absence of CDK12 in oocyte leads to female infertility

This study shows that CDK12 absence in oocytes causes female infertility by disrupting transcriptional activity and maternal transcriptome maintenance.

Transcriptomic analysis of whole staged ovarian follicles reveals stage-specific folliculogenesis signatures in mice

This study uses single follicle RNA-seq to identify transcriptional signatures of ovarian follicles in mice, revealing stage-specific gene pathways involved in folliculogenesis.

Rediscovering the Rete Ovarii: a secreting auxiliary structure to the ovary

The paper characterizes the fetal development and function of the rete ovarii, identifying it as a secretory structure with potential roles in ovarian function and homeostasis.

Acute high-dose irradiation of human primary ovarian cells reveals a shift in transcriptomics profile and impairment in cell-cell adhesion

This study examines how human ovarian cortical and medullary cells respond to acute X-ray exposure, finding transcriptomic shifts and impaired cell-cell adhesion.

FOSL2 regulates gonadotropin-dependent folliculogenesis through feedback amplification of FSH/FSHR signaling

This study identifies FOSL2 as a key regulator of FSH/FSHR signaling in folliculogenesis, showing it enhances FSHR transcription and forms a self-amplifying loop critical for reproductive function.

Blastocoel fluid RNA predicts pregnancy outcome in assisted reproduction

The paper predicts pregnancy outcomes in assisted reproduction by analyzing RNA from blastocoel fluid, showing higher RNA levels correlate with implantation failure.

Regulation of Female Reproductive Aging by the Spag17 Gene

This study identifies Spag17 as a key modulator of female reproductive aging, showing its role in maintaining fertility and preventing age-related complications.

A Novel mTOR-Stat3-Stathmin Pathway Establishes Oocyte Polarization Competence by Orchestrating Centrosome Regulation and Microtubule Organization

The paper identifies a novel mTOR-Stat3-Stathmin pathway that regulates oocyte polarity through centrosome and microtubule organization in zebrafish.

Dysregulation of the actin cytoskeleton and FMRP causes polar body protrusion defects in human fragile X premutation and aged oocytes

Dissecting PADI6 function defines oocyte cytoplasmic lattices as regulatory hubs for fundamental cellular processes

The paper investigates PADI6's role in oocyte cytoplasmic lattices using single-cell proteomics and transcriptomics, revealing its critical scaffolding function and connections to other oocyte compartments.

Hyper-ovulation in an ovarian-specific transgenic mouse model.

The paper generates an ovarian-specific FLAG-ICER-II(C) transgenic mouse model and shows it results in hyper-ovulation upon gonadotropin stimulation.

A simple immunohistochemical method for perinatal mammalian ovaries revealed different kinetics of oocyte apoptosis caused by DNA damage and asynapsis.

The paper introduces a simple squash method for immunohistochemical analysis of perinatal mouse ovaries, revealing different kinetics of oocyte apoptosis caused by DNA damage and asynapsis.

Minipuberty regulates reproductive lifespan and ovarian follicular loss in a mouse model with reduced minipubertal gonadotropin levels

This study shows that reducing minipubertal gonadotropin levels in mice extends reproductive lifespan but accelerates ovarian follicle depletion without affecting early fertility.

Mammalian oocytes receive maternal-effect RNAs from granulosa cells

The paper shows that growing mouse oocytes receive mRNAs from granulosa cells, which are enriched for transcripts important for oocyte maturation and early embryo development.

Intense oocyte competition builds the female reproductive reserve in mice

The paper uses live imaging and single-cell sequencing to show that oocyte competition in mice enhances quality through autophagy-driven debris absorption, essential for fertility.