Pluripotent Stem Cells Research

This study identifies a common molecular logic governing embryonic stem cell self-renewal and reprogramming through Boolean network analysis and experimental validation.

This study shows that partial reprogramming reduces epigenetic age in human cells but decouples it from the loss of somatic identity, suggesting a safe window for rejuvenation without teratoma risk.

The paper analyzes genetic integrity in iPSCs from the ForIPS consortium, identifying somatic variants and highlighting the need for individual clone evaluation.

This study induces transient cell reprogramming in mouse skeletal muscle using plasmid DNA to enhance tissue regeneration after injury.

The paper shows that a TALE-based repressor targeting Xist intron 1 enhances iPSC generation and SCNT embryo development in mice, without affecting Xist expression.

This study compares endoderm differentiation in humans and chimpanzees using iPSCs, finding high conservation of gene regulatory trajectories despite species-specific expression differences.

The paper compares gene expression and regulatory differences between human and chimpanzee iPSC-derived cardiomyocytes and primary heart tissues to assess their utility for comparative genomic studies.

This study identifies key regulators of the transition from pluripotency to germ cell fate using CRISPR screening in PGC-like cells, highlighting Nr5a2 and Zfp296 as critical factors.

The paper shows that inhibiting Kat2a increases transcriptional heterogeneity in mouse embryonic stem cells, leading to reduced pluripotency and accelerated differentiation.

This study shows that hyperpolyploid mouse embryos can develop into blastocysts, and aggregation recovers pluripotent cells in hexadecaploid embryos.

The paper shows that OCT4 inheritance predetermines fate choice in human embryonic stem cells, with higher OCT4 ratios in daughters leading to sustained pluripotency and reduced differentiation potential.

This study describes a method to generate functional human peripheral sensory neurons from hiPSCs that release substance P in response to TRPV1 agonists.

The paper shows that a genetic variant linked to mental illness reduces TRANK1 expression in neural cells, which is reversed by valproic acid treatment.

This study identifies the Gatad2a-Chd4-Mbd3 subcomplex of the NuRD complex as a key inhibitor of naive pluripotency, showing that its disruption enables deterministic reprogramming of somatic cells to iPSCs.

The paper identifies FACT as a conserved reprogramming barrier in C. elegans and humans that maintains cell identity by modulating chromatin accessibility.

This paper introduces a simple, inexpensive method to generate kidney organoids from human pluripotent stem cells, showing they develop tubular structures by day 8 and resemble fetal kidney structures by day 14.

This study analyzes the mutational burden in human iPSCs using whole genome sequencing and integrates transcriptome and epigenome data to identify mutation patterns and their impact on gene expression.

This paper introduces a standardized, feeder-free protocol to efficiently produce erythroid, megakaryoid, and myeloid cells from single-cell-derived iPSC colonies without pre-purification or transcription factor overexpression.

This study develops an in vitro protocol to recapitulate early paraxial mesoderm development in mouse ES cells, showing efficient differentiation into muscle precursor cells and functional muscle fibers.

The paper develops a mouse pluripotent stem cell micropattern system to study regionalized fate specification and patterning, revealing how signals direct distinct identities and spatial organization.

This study compares the differentiation potential of Brachyury+ mesodermal cells from 3-D and 2-D culture systems, finding differences in gene expression and ex vivo differentiation outcomes.

The paper develops a protocol to generate human neuromesodermal and spinal cord progenitors in vitro, validates it with RNA-Seq, and identifies new pathways in neural differentiation.

This study compares the ability of mouse ES cell-derived progenitors to generate NMp behavior in vivo, finding that NMp competence is lost in anterior gastruloids.

This study shows that GSK3 inhibition prevents multilineage maturation of hPSC-derived lung progenitors in 3D culture, while NOTCH and WNT signaling influence proximodistal cell fate specification.

The paper identifies concordant transcriptional signatures of schizophrenia in hiPSC-derived neurons and post mortem brains, suggesting neurons better model genetic risk and recommends increasing cohort size in hiPSC studies.

This study induces and engrafts human pluripotent stem cell-derived lung bud tip progenitor-like cells in vitro and in vivo, showing their similarity to native lung tissue and multi-lineage potential.

The paper shows OCT4 prevents cell fate change by blocking MITF's effects in embryonic stem cells and other differentiated cells.

This study compares two mouse motor neuron differentiation protocols, showing that direct programming can reach the same final state through a different intermediate state with distinct gene expression.

The paper shows that FGF/ERK inhibition during a short time window can convert ICM cells into epiblast, requiring transcription and proteasome degradation.

This paper models cell reprogramming as transitions between hierarchically related cell cycle states, identifying two mechanisms that effectively drive reprogramming.