Advanced Fluorescence Microscopy Techniques

Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy.

This paper demonstrates that self-blinking dye JF635b improves nanobody performance in single-molecule localization microscopy, enabling reliable imaging across multiple modalities with high precision.

Single-dye, transfection-free FLIM multiplexing via bioorthogonal chemistry

The paper demonstrates a method to multiplex FLIM using small molecule probes modified with bioorthogonal chemistry to achieve distinct fluorescent lifetimes without transfection.

Development of a photostable pH biosensor based on mStayGold

The paper develops a photostable pH biosensor called serapH using mStayGold as a scaffold and introduces a screening method for pH-sensitive FPs in bacterial colonies.

BLeaching In-cell Single-molecule burstS (BLISS) reveals dynamic fraction of HP1α clusters in chromocenters of undifferentiated embryonic stem cells

BLISS reveals dynamic HP1α clusters in chromocenters of undifferentiated ESCs, showing they are hyperdynamic and have lower fluorescence lifetimes than FLIM-averaged values.

Dodecagon light-sheet fluorescence microscopy for large-volume imaging without striping artifacts

This paper introduces dodecagon light-sheet fluorescence microscopy (dodecaLSFM) to eliminate striping artifacts in large-volume imaging, demonstrating its effectiveness in imaging whole mouse brain vasculature and combining with expansion microscopy for 3D cellular resolution.

Beyond Navigation - Tissue-contacting Fluorescent Lifetime Imaging reveals a pathology-linked lung cancer phenotype at the point of biopsy

The paper validates a tumour-associated phenotype identified by FLIM in lung cancer biopsies, showing it is reproducible across imaging systems and distinguishable from normal tissue.

Spatially pooling photon information enables photon-efficient quantitative imaging

This paper introduces a method to recover spatially distributed photon information for quantitative imaging, improving precision in low-photon regimes.

Toolbox for fluorescent labelling of Pseudomonas aeruginosa across scales: from single cells to bacterial communities and host infection models

The paper develops a multicolor fluorescent labelling toolkit for Gram-negative bacteria, enabling reliable imaging and analysis across scales in host-pathogen contexts.

In vivo optical clearing of the mouse brain

This study develops a biocompatible clearing agent for live mouse brain tissue, enabling deeper optical imaging with reduced laser intensity.

Expression of photoactivatable molecules enables FCS in live cells by controlling fluorescence intensity

The paper develops a method to measure nucleoplasmic material properties in live fission yeast cells using photoactivatable molecules and FCS, finding no significant variation during the cell cycle.

SynthMLM: A framework for interpretable analysis and synthetic localisation data generation for SMLM

SynthMLM generates synthetic localisation data for SMLM using interpretable structural descriptors from experimental data, enabling benchmarking and testing of analysis methods.

ImpRes: A robust FRAP framework to quantify fast diffusion of cytoplasmic probes

The paper introduces a robust FRAP framework, ImpRes, to accurately quantify fast cytoplasmic diffusion by addressing noise, normalization, and spatial restrictions through a single-equation fitting approach.

A non-toxic, user-friendly buffer that enhances green fluorophore performance in conventional and super-resolution imaging

The paper introduces a non-toxic buffer that enhances green fluorophore brightness and photostability in both conventional and super-resolution imaging.

Unlocking subcellular imaging of a cnidarian photosymbiont Breviolum minutum , through expansion microscopy

This paper develops an expansion microscopy workflow to visualize subcellular features of Breviolum minutum, a cnidarian photosymbiont, overcoming challenges posed by autofluorescence and small size.

Probing intracellular physical environments by rotational and translational single-particle tracking

This paper introduces a method combining rotational and translational single-particle tracking to analyze nanoscale dynamics, showing it reveals distinct biophysical features of cellular compartments.

Experimental and simulated FRAP for the quantitative determination of protein diffusion in helical cells

The paper develops a framework for quantitatively determining protein diffusion in helical cells using FRAP simulations and experiments, finding similar diffusion rates in helical and rod-shaped bacteria.

Space-Time Light-Sheet Microscopy

This paper introduces space-time light-sheet microscopy (ST-LSM), a single-objective technique that improves imaging volume and field of view while maintaining resolution.

GIAnT: a Glutamate Imaging Analysis Toolbox

GIAnT is a toolbox for analyzing glutamate imaging data that corrects motion, identifies active synapses, and improves signal quality.

ELDR-Glo, a biosensor for cell age that correlates with quiescence depth

ELDR-Glo is a fluorescent biosensor that estimates relative cell age and quiescence depth, functioning in both live and fixed cells.

Calibration-free single-frame super-resolution fluorescence microscopy

This paper introduces a deep-learning method for super-resolution fluorescence microscopy that reconstructs images from a single camera frame without calibration or long acquisition times.

Imaging the voltage of neurons distributed across entire brains of larval zebrafish

The paper presents a remote scanning light-sheet microscope for whole-brain voltage imaging in larval zebrafish, revealing spatial patterns of neuronal activity during visual stimuli and spontaneous bursts.

Enhanced 2D structured illumination microscopy: super-resolution with optical sectioning and reduced reconstruction artifacts

This paper introduces enhanced 2D-SIM to reduce reconstruction artifacts and improve resolution in thick samples using optimized coarse and fine patterns.

aDISCO: A clearing method to enable 3D microscopyof large archival paraffin-embedded human tissue blocks

aDISCO enables 3D microscopy of large archival FFPE human tissue blocks, revealing subtle pathologies in focal cortical dysplasia through deep-learning analysis.

Fast calcium-dependent fluorescent labeling for recording of neuronal activation

The paper introduces faster variants of the Caprola tool for calcium-dependent fluorescent labeling, enabling more sensitive and efficient recording of neuronal activity in zebrafish.

Genetic encoding of 3-cyano-tyrosine and its use in controlling the chromophore isomeric state of the fluorescent protein mKate

This paper genetically encodes 3-cyano-L-tyrosine into a fluorescent protein, altering its chromophore isomerization state and spectral properties.

Spectroscopic DNA-PAINT for simultaneous multiplexed super-resolution microscopy

This paper introduces sDNA-PAINT, a method that improves spectral precision and multiplexing in super-resolution microscopy using DNA Origami Nanorulers and spectroscopic SMLM.

Super-Resolution Optical Sectioning Microscopy Visualizes Nanopores in the Plasma Membrane of Endothelial Cells in situ

The paper visualizes nanopores in endothelial cells in situ using a super-resolution microscope after staining with a fluorescent dye.

Balancing performance and complexity of dual-wedge prism-based spectroscopic single-molecule localization microscopy

This study compares DWP-based sSMLM configurations to balance localization and spectral precision with experimental complexity.

Recovering membrane interaction kinetics of single molecules from 3D tracking data

This paper develops a method to measure membrane interaction kinetics of single molecules in live bacteria using 3D tracking data and hidden Markov modeling.

A Lightweight Deep Learning Framework for Fast, Real-Time Super-Resolution Fluctuation Imaging

This paper introduces RESURF, a lightweight deep learning framework that enables real-time super-resolution fluctuation imaging with improved temporal and spatial resolution.