Advanced Fluorescence Microscopy Techniques

Intronised fluorophores improve reporter output and biosensor expression in plants

This study develops intronised fluorophores to enhance reporter output and biosensor expression in plants, showing increased fluorescence and improved stability in various contexts.

Biosensor-enabled single-molecule enzyme activity profiling for function-based molecular counting

This paper introduces a biosensor-based method for single-molecule enzyme activity profiling without enzyme-specific substrates, enabling absolute quantification of active enzyme molecules in cellular and circulating samples.

Rapid volumetric reconstruction and tracking for Fourier light-field microscopy enables real-time calcium imaging in freely behaving Hydra.

This paper introduces OpenFLR, a software framework for real-time volumetric reconstruction and calcium imaging using Fourier light-field microscopy in Hydra.

Independent noise realizations enable morphologically agnostic image reconstruction in single-photon-sensitive microscopy

This paper introduces a morphology-agnostic regularization framework for image deconvolution in single-photon-sensitive microscopy, eliminating semi-convergence and improving reconstruction quality.

Fully Two-Photon-Driven RESOLFT Microscopy for Super-Resolution Imaging inside Tissue

This paper introduces 2P-RESOLFT microscopy, achieving 205-nm lateral resolution to visualize previously unreported membrane protrusions in pancreatic beta cells.

Physicochemical balancing facilitates fast cyclic immunolabeling in centimeter-scale FFPE tissues

The paper introduces FIDELITY, a method for rapid cyclic immunolabeling of FFPE tissues, enabling multi-cycle protein profiling in intact specimens.

An All-Optical Approach to Probe Chloride Transport with a Bright ChlorON

The paper introduces an improved fluorescent protein indicator, ChlorON-1-PRO, for real-time imaging of chloride transport in cells.

Broadband backscattering confocal microscopy enables label-free 3D live cell nanoscale sensitive imaging

This paper introduces broadband backscattering confocal microscopy (BBCM) for label-free 3D live cell imaging with improved contrast and size sensitivity.

A custom two-in-one HIST and line-scanning confocal excitation module

This paper introduces a reconfigurable microscopy module that combines HIST and line-scanning confocal techniques for versatile fluorescence imaging.

Multiphoton tomographic fluorescence lifetime imaging microscopy -TomoFLIM

This paper introduces TomoFLIM, a high-speed multiphoton FLIM technique that enables rapid, accurate imaging of dynamic biological processes with compression ratios over 90%.

QuantiTrack: A unified software to study protein dynamics in living cells

QuantiTrack, a MATLAB GUI, unifies single-molecule detection, tracking, quality control and mobility analyses; for glucocorticoid receptor, hormone washout cut bound fraction, dwell times and low-mobility occupancy.

Dual-Mode Bio-CM2: Multimodal Computational Miniature Mesoscope for Fluorescence and Label-Free Imaging

The paper introduces dual-mode Bio-CM2, a miniature mesoscope that captures fluorescence and label-free imaging simultaneously, demonstrating its use across different organisms and biological contexts.

PSF-Driven Spatio-Temporal Blending in Fluorescence Lifetime Imaging Microscopy and Its Mitigation via Mean-Shift Super-Resolution-Based Masking.

This paper introduces a method to mitigate spatio-temporal blending in FLIM by using MSSR-based masking, improving spatial resolution while preserving lifetime accuracy.

Miniscope Zero: a fully wireless, single-cell-resolution miniature microscope for imaging neural dynamics in freely behaving animals

Live-cell PAINT microscopy of protein targets via fluorogenic exchangeable HaloTag ligands

The paper introduces a method for live-cell super-resolution microscopy using fluorogenic HaloTag ligands and BF dyes, enabling single-molecule localization and diffusivity mapping of proteins.

Single-shot light-field microscopy captures delivery-dependent dye and autofluorescence patterns in Caenorhabditis elegans

This paper uses light-field microscopy to rapidly capture 3D fluorescence patterns in C. elegans without sequential z-scanning, showing how dye delivery affects probe distribution.

Processing, analysing and modelling kinetic data in the era of high-throughput single-molecule biophysics

The paper provides tools and methods for analyzing high-throughput single-molecule biophysics data using first-passage time and maximum likelihood estimation to model kinetic behavior.

PURELIGHT: a quantitative photon-counting framework unifying intensity and lifetime imaging at video rate across detector technologies

PURELIGHT improves photon efficiency and accuracy in fluorescence microscopy by recovering undistorted intensity and lifetime measurements at high count rates across multiple detector technologies.

The enlightened entomologist: fast, non-destructive whole-arthropod clearing for three-dimensional imaging

This paper introduces a fast, non-destructive clearing method for arthropods to enable 3D imaging using existing microscopes.

A Nanoheater-Integrated Fluorescence Lifetime Thermometer for Investigating Subcellular Heat Shock Factor 1 Responses

This paper develops a nanoheater-thermometer to measure subcellular temperature and monitor HSF1 responses in living cells, showing that localized heating induces HSF1 foci at lower temperatures than whole-cell heating.

Combined Image-Based Profiling and Biochemical Analysis of GCaMP Overexpression Effects on Mammalian Cells

The paper uses imaging and biochemical methods to analyze the effects of GCaMP overexpression on mammalian cells, identifying cellular morphology changes and cytoskeletal interactions.

iClima: a ratiometric genetically encoded sensor for optical imaging of fast GABAergic chloride currents in vitro and in vivo

The paper introduces iClima, a ratiometric genetically encoded sensor for imaging GABAergic chloride currents, showing it can detect Cl- transients in vitro and in vivo with improved photostability and pH independence.

Autofluorescence lifetime imaging resolves cell heterogeneity within peripheral blood mononuclear cells

This paper demonstrates that autofluorescence lifetime imaging can identify immune cell subsets and activation states in PBMCs with high accuracy, enabling label-free metabolic analysis.

Characterizing particle dynamics in live imaging through stochastic physical models and machine learning

This paper develops a machine learning method to classify subdiffusive particle dynamics in live imaging, showing it can distinguish different motion behaviors and reveal how receptor dynamics relate to HIV-1 activity.

Wide-Field Calcium and Flavoprotein Autofluorescence Imaging in Living Mice

This paper describes protocols for chronic transcranial wide-field imaging in mice using calcium and flavoprotein signals to study cortical activity and metabolic dynamics.

Counting fluorescent emitters with a single photon avalanche diode array

This paper introduces a method using SPAD arrays to count fluorophores in widefield microscopy by modeling PCH statistics and performing Bayesian inference.

Spatially patterned, spectral single-molecule microscopy

This paper introduces a new spectral single-molecule microscopy technique using spatially patterned detectors, achieving spectral and positional resolution without complex optical components.

Extending conventional TIRF microscopy to image single molecules in micromolar analyte backgrounds

This paper extends conventional TIRF microscopy to image single molecules in micromolar analyte backgrounds, enabling studies of weak biomolecular interactions.

Whole-organ surface mapping using multiview projection reconstruction

This paper introduces PRISM, a method for whole-organ surface mapping using multiview projection reconstruction, enabling efficient and accurate surface feature analysis in biological samples.

vFLIM: Machine Learning-enabled Light Sheet Fluorescence Lifetime Imaging

The paper introduces a machine learning-enabled pipeline for volumetric FLIM imaging in living systems, enabling long-term and high-speed data acquisition and analysis.