Advanced Fluorescence Microscopy Techniques

Epitope-based labeling for improved live-imaging of endogenous proteins in C. elegans

The paper evaluates and combines four epitope-based labeling systems for live-imaging of endogenous proteins in C. elegans, showing their effectiveness and advantages over direct labeling.

Structural cell biology by mega-expansion microscopy

The paper introduces Mega-expansion microscopy, a fluorescence imaging technique that enables high-resolution structural visualization of whole cells using conventional microscopes.

Using Latent Chemical Recognition in an Evolved Periplasmic Binding Protein Family to Diversify Biosensors

The paper screens a family of evolved biosensors against diverse ligands to identify cross-reactive compounds, finding multiple ligands beyond neural drugs that activate the sensors.

Endomicroscopic fluorescence lifetime imaging enables molecular detection and targeted sampling in the distal human lung

This paper demonstrates the use of endomicroscopic fluorescence lifetime imaging with targeted probes to detect bacteria and neutrophils in the distal human lung, enabling real-time molecular imaging and sampling.

Fluorescent Protein Photobleaching: From molecular processes to spectromicroscopy

This paper introduces a quantitative workflow to study FP photobleaching by combining live-cell measurements with in vitro spectroscopy, revealing complex chemical pathways and photoproducts affecting fluorescence intensity and lifetime.

Depth-Dependent Advantages of Three-Photon Microscopy for Imaging Through Intact Murine Cortical Bone and Into the Marrow

This study compares two-photon and three-photon microscopy in ex vivo mouse bones, finding 3P provides better image quality beyond 50 micrometers and enables visualization of marrow cells through intact bone.

A workflow for the identification of oligomeric structures on tilted sample planes in Cryo-SMLM

This paper introduces a workflow to identify oligomeric structures in Cryo-SMLM by accounting for tilted sample planes and demonstrates its accuracy on synthetic data.

Nanoscale rheological heterogeneity revealed by Single Particle orientation Tracking (SPoT) of ultrashort carbon nanotubes in brain tissue

This paper introduces SPoT, a method to track carbon nanotubes in brain tissue to study nanoscale rheological heterogeneity by analyzing translational and rotational dynamics.

Simultaneous, real-time tracking of many neuromodulatory signals with Multiplexed Optical Recording of Sensors on a micro-Endoscope

This paper develops a micro-endoscope probe to simultaneously track multiple neuromodulators in real time using fluorescent sensors, demonstrating its use in brain slices and awake mice.

CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies

CALIPERS enables cell cycle-aware live imaging by integrating a modified FUCCI variant, analysis software, and reporter lines for phenotyping and regeneration studies.

Plug-and-Play 3D localization microscopy

This paper demonstrates a simple 3D single-molecule localization microscopy technique using 3D-printed phase masks and computational compensation.

High Axial Resolution Is Necessary for Quantitative Two-Photon Calcium Imaging of Neuronal Populations

The paper shows that low axial resolution in two-photon calcium imaging corrupts neuronal activity measurements and that no analysis pipeline fully corrects these issues.

Quantitative evaluation of LED based optical autofocus module

The paper evaluates an LED-based optical autofocus module using 2D autocorrelation analysis and demonstrates its axial stability over time.

Bio-CM{superscript 2}: Distributed computational optics for cortex-widecellular imaging

Four miniature lenses sharing one CMOS sensor, with computational stitching, give cellular-resolution imaging over 7.5 × 10 mm^2 at video rate, resolving 3,474 mouse cortical neurons.

Spike inference from calcium imaging data acquired with GCaMP8 indicators

This study evaluates and optimizes spike inference methods for GCaMP8 calcium imaging data, showing improved detection of high-frequency spikes and real-time activity.

Compact red-shifted near-infrared fluorescent proteins enable deep-tissue SWIR imaging with in vivo optical clearing.

The paper develops red-shifted near-infrared fluorescent proteins for deep-tissue SWIR imaging, showing improved signal and imaging depth in vivo.

Programmable protein reference standards for benchmarking sub-10-nm fluorescence microscopy

This paper introduces programmable protein standards for benchmarking sub-10-nm fluorescence microscopy, demonstrating their utility in assessing fluorophore interactions and imaging performance.

Multi-channel high-density single-molecule localization

This paper introduces DECODE-Plex, a deep learning framework for multi-channel single-molecule localization microscopy, improving imaging speed and quality in dense, overlapping data.

Dynamic Extraction and Tracking of Emitted Cellular Transients resolves low-salience fluorescence events

DETECT improves detection of low-salience fluorescence events by combining background suppression, classification, and tracking, enabling analysis of neuromodulatory activity.

Whole human organ clearing and multimodal mapping

This paper introduces mCleLight, a method for clearing, labeling, and imaging whole human tissues, enabling multiscale analysis of organ architecture.

Resolving FRET Signal Degeneracy and Population Heterogeneity via Bayesian Nonparametrics

This paper introduces BNP-FRET-Bin to resolve FRET signal degeneracy and population heterogeneity by inferring state numbers and kinetics directly from FRET data, revealing new biologically relevant states.

iG6PSnFR: A genetically encoded fluorescent sensor for observing glucose-6-phosphate dynamics in living preparations

The paper develops and validates a genetically encoded fluorescent sensor, iG6PSnFR, for measuring glucose-6-phosphate dynamics across multiple biological contexts.

Adapting Upright Light Sheet Fluorescence Microscopy for Imaging at Air-Liquid Interface

This paper presents a device for adapting upright light sheet fluorescence microscopy to image samples at air-liquid interface, demonstrating its use on three biological specimens.

Thousandfold Expansion Microscopy

A four-network sodium acrylate/dimethylacrylamide hydrogel, cast ionic-within-ionic without neutral re-embedding, reaches about 1000-fold linear expansion and resolves individual peptide residues on standard confocal microscopes.

Real-Time Axial Motion Compensation for Intravital Two-Photon Imaging of Mechanically Loaded Bone

This paper develops a real-time axial motion compensation system for intravital two-photon imaging of mechanically loaded bone, improving image quality and data reliability.

A structured-illumination miniscope for optically sectioned imaging and real-time neural decoding

This paper introduces a structured-illumination miniscope for high-contrast calcium imaging in freely behaving mice, demonstrating improved signal quality and real-time neural decoding.

Mapping absolute membrane voltage using dynamic photocycle control

This paper introduces VMAP, a method for absolute membrane voltage imaging, and demonstrates its application in measuring drug effects, bioelectric patterns, and 3D voltage maps in zebrafish embryos.

Fluorescence cross-correlation spectroscopy quantifies affinity, cooperativity, and kinetic stability in ternary protein complexes

This paper uses FCCS to quantify affinity, cooperativity, and kinetic stability in ternary protein complexes, showing cooperative stabilization of the HER2-trastuzumab-pertuzumab complex.

FENNEC: photon-level deep learning for classifying bursts in diffusion-based single-molecule FRET

FENNEC classifies bursts in diffusion-based smFRET data into conformational dynamics and photophysical artefacts using simulated data, without requiring labeled experimental data.

From background to foreground: secondary antibodies coupled to lipophilic ATTO dyes enable high-density membrane labeling in super-resolution and expansion microscopy

This paper introduces secondary antibodies conjugated to lipophilic ATTO dyes for high-density membrane labeling in super-resolution and expansion microscopy, enabling precise organelle visualization.