Animal Genetics and Reproduction

Short-term gonadal cultures are sufficient for germline transmission in a songbird

This study shows that short-term gonadal cultures of zebra finch PGCs can produce germline chimeras and validated offspring, despite being heterogeneous, unlike long-term chicken PGC cultures.

Identification of transgene insertion sites via short- and long-read whole genome sequencing.

This study presents two whole genome sequencing pipelines to identify and genotype transgene insertion sites in transgenic mice, enabling accurate zygosity determination and breeding strategies.

Germline-mediated ubiquitous recombination in ScxCre male mice: implications for tendon research

The paper shows that ScxCre mice cause unintended recombination in male germline, leading to global gene effects in offspring, and advises sex selection to avoid off-target effects.

Rapid Derivation of Cloning-Competent Cells from Peripheral Blood Advances Conservation Biobanking

This study shows that blood-derived endothelial progenitor cells and pericytes can be used to create viable cell lines faster and more reliably than fibroblasts for conservation biobanking in endangered species.

The chicken retrovirus-like gene ENS-1/ERNI and its LTR Soprano are involved in primordial germ cell development.

The paper identifies ENS-1/ERNI and its LTR Soprano as factors influencing chicken primordial germ cell development and in vitro establishment efficiency.

Long-Term Rock Dove (Columba livia) Primordial Germ Cell Culture: A Tool for Avian Conservation

This study establishes a long-term culture system for rock dove primordial germ cells, enabling germline manipulation and conservation applications.

Evidence that genome editing is preferable to transgenesis forenhancing animal traits

This study compares transgenesis and genome editing across 72 species and 55 genes to assess their effectiveness in enhancing animal production traits, finding gene editing more impactful overall.

Minos-mediated transgenesis in the pantry moth

The paper demonstrates that Minos transposase can achieve robust germline transgenesis in Lepidoptera, offering an alternative to piggyBac for genetic modification.

One-step generation of modified cattle and sheep from spermatid-like haploid stem cells

This study generates gene-modified cattle and sheep using haploid stem cells with a modified iCHI approach that enhances embryo development.

Production of Fc-fused receptor agonists for glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide (GLP-1/GIP) in the milk of transgenic mice

This paper demonstrates the production of GLP-1/GIP receptor agonists in transgenic mice milk, showing bioactivity and potential for scalable biologics manufacturing.

Parental origin of transgene determines recombination efficiency in GFAP-creERT2 mice

The paper shows that the parental origin of the hGFAP-creERT2 transgene affects recombination efficiency in mice, with paternal inheritance leading to robust recombination and maternal inheritance causing little to none.

Generation of a Transgenic Mouse Model for Investigating Mitochondria in Sperm

The paper generates transgenic mice expressing mitochondrial-targeted GFP to study sperm mitochondria function.

Generation of the First Transgenic Line of the Iconic Coral Reef Fish Amphiprion ocellaris

This paper generates the first transgenic line of the clownfish Amphiprion ocellaris using the Tol2 transposon system, achieving a 2.7% transgenesis rate and confirming germline transmission.

Stable germline transgenesis using the Minos Tc1/mariner element in the sea urchin, Lytechinus pictus.

This paper establishes the first germline transgenic sea urchin using the Minos transposon, enabling stable transgene integration and inheritance.

First gene-edited calf with reduced susceptibility to a major viral pathogen

The paper describes the creation of a gene-edited calf with reduced susceptibility to BVDV through CD46 modification, showing no adverse effects and confirming the effectiveness of the approach.

Efficient transfection of Atlantic salmon primary hepatocyte cells for functional assays and gene editing

This paper develops a method for efficiently transfecting Atlantic salmon primary hepatocytes for functional assays and gene editing.

Direct cryopreservation of poultry/avian embryonic reproductive cells: A low-tech, cost-effective and efficient method for safeguarding genetic diversity

The paper describes a low-tech method for cryopreserving chicken embryonic gonads to conserve genetic diversity for poultry breeding.

Surrogate production of genome edited sperm from a different subfamily by spermatogonial stem cell transplantation

This study demonstrates the successful production of genome-edited sperm from a phylogenetically distant fish species using cross-subfamily spermatogonial stem cell transplantation.

Improving germline transmission efficiency in chimeric chickens using a multi-stage injection approach

This study improves germline transmission efficiency in chimeric chickens using a multi-stage injection approach, achieving a 13.6% germline transmission rate with double injection.

A resource of targeted mutant mouse lines for 5,061 genes

The paper reports the generation of over 5,000 new mouse mutant strains for targeted genes, significantly expanding the available genetic resources for biomedical research.

Highly efficient genome modification of cultured primordial germ cells with lentiviral vectors to generate transgenic songbirds

This study generates transgenic songbirds by efficiently modifying zebra finch primordial germ cells with lentiviral vectors.

Single generation allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating

This paper introduces a method to transfer genetic alleles between chicken breeds using SDS mating to create pure breeds with desired traits.

Generation of multi-transgenic pigs using PiggyBac transposons co-expressing pectinase, xylanase, cellulase?β-1.3-1.4-glucanase and phytase

This paper describes the generation of multi-transgenic pigs expressing digestive enzymes to improve feed efficiency and reduce environmental impact.

Testes of DAZL null sheep lack spermatogonia and maintain normal somatic cells

The paper generates DAZL-null sheep that lack germ cells but have normal somatic cells, demonstrating the feasibility of using absolute donor germline transmitters in livestock breeding.

Polyploidy of semi-cloned embryos generated from parthenogenetic haploid embryonic stem cells

This study generates fertile semi-cloned mice using parthenogenetic haploid embryonic stem cells with deleted DMRs, revealing frequent polyploidy in resulting embryos.

Transgenic goats producing an improved version of cetuximab in milk

This study generates transgenic goats producing an improved cetuximab in milk, showing enhanced therapeutic properties and safety compared to existing treatments.

Targeted demethylation of H3K9me3 and H3K36me3 improves somatic cell reprogramming into cloned preimplantation but not postimplantation bovine concepti

This study shows that targeted demethylation of H3K9me3 and H3K36me3 in bovine fibroblasts improves cloning to preimplantation embryos but not to adult survival.

Establishment of Etv5 gene knockout mice as a recipient model for spermatogonial stem cell transplantation

This study establishes Etv5 gene knockout mice as a recipient model for spermatogonial stem cell transplantation, showing partial success in germ cell restoration but low efficiency in full spermatogenesis.

Somatic cell nuclear transfer in non-enucleated goldfish oocytes: understanding DNA fate during meiosis resumption and first cellular division.

The paper investigates DNA fate during meiosis resumption and first cell division in goldfish clones using non-enucleated oocytes, finding that maternal DNA is often fragmented and somatic DNA forms abnormal spindles.

Large-scale discovery of mouse transgenic integration sites reveals frequent structural variation and insertional mutagenesis

This study identifies insertion sites in 40 transgenic mouse lines, revealing frequent disruptions of endogenous genes and structural variations.