Animal Genetics and Reproduction

Long-read PacBio HiFi sequencing resolves the genomic integration site and structure of the Nestin-CreERT2KEisc/J transgene

The paper uses PacBio HiFi sequencing to determine the genomic integration site and structure of the Nestin-CreERT2KEisc/J transgene, revealing deletions and enabling zygosity testing.

A Cell Viability and Utility Index (CVUI) for wildlife fibroblast biobanking: framework development and preliminary empirical validation

The paper introduces the Cell Viability and Utility Index (CVUI) to assess cell culture quality in wildlife biobanking, validated across 154 culture rounds from 46 species.

Initiating mammalian reproduction from a single male genome

This paper demonstrates a method to induce male-to-female sex reversal in mice using Y-chromosome elimination and somatic cell nuclear transfer, enabling reproduction from a single male genome.

CRISPR-mediated engineering of bovine satellite cells for Alpha-Gal Syndrome-compatible cultivated meat

This paper uses CRISPR/Cas9 to edit bovine satellite cells to remove the alpha-gal epitope, creating AGS-compatible cultivated meat with reduced allergenic potential.

The world's first cloned golden wild yak via interspecific SCNT: 4800m donor origin and 4200m vitrified blastocyst transfer

This paper reports the first successful cloning of a golden wild yak using interspecific somatic cell nuclear transfer under extreme high-altitude conditions.

Efficient Generation of Humanized Cloned Cattle via Embryonic Stem Cell-Mediated Dual-Round Site-Specific Editing

This study develops a method using bovine epiblast stem cells for efficient gene editing and cloning of cattle, resulting in higher birth and survival rates compared to fibroblast-based cloning.

Ataxia-Telangiectasia Mutated Inhibition Enhances Adeno-Associated Virus-Mediated Knock-in Efficiency in Pig Zygotes

The paper shows that inhibiting ATM with AZ32 improves AAV-mediated long-fragment knock-in efficiency in pig zygotes, enabling double knock-in at two loci.

Flexible oocyte manipulation with delayed maturation and improved SCNT efficiency using induced pluripotent stem cells

This study improves SCNT efficiency in cattle by delaying oocyte maturation and using biPSCs as donor nuclei, resulting in higher fusion and blastocyst rates.

Application of MT3 in the Development and Immunogenicity Evaluation of GnRH Vaccines

This study constructs and evaluates a GnRH vaccine using MT3, showing it induces antibodies and reduces hormone levels in mice, leading to testicular changes associated with immune castration.

Hybrid yak-cattle in situ conservation via interspecies somatic cell nuclear transfer at ultra-high-altitude region

The paper reports the successful cloning of a hybrid yak-cattle (Pien-niu) via interspecies somatic cell nuclear transfer at an ultra-high-altitude region, demonstrating its viability for in situ conservation.

Targeted sex ratio distortion in the mouse

The paper describes a method to achieve targeted sex ratio distortion in mice using a selfish supergene component, resulting in a high prevalence of non-transgenic offspring of the desired sex.

Establishment of a Reverse Genetics System for the Study of Human Immune Functions in Mice

This paper establishes a method to generate humanized mice with targeted gene knockouts in the human immune system using Cas9-edited CD34+ cells.

Efficient Production of Pseudopregnant Female Mice Using Non-Estrous Individuals and the Whitten Effect

This study develops a method to produce pseudopregnant female mice using non-estrous individuals and the Whitten effect, achieving high copulation and embryo transfer success rates.

Comparative analysis of trans-chromosomic rodent models reveals improved somatic hypermutation and class-switch recombination in rats

The paper compares trans-chromosomic rodent models and finds rats show improved somatic hypermutation and class-switch recombination compared to mice.

The widely used Ucp1-CreEvdr transgene elicits complex developmental and metabolic phenotypes.

The paper analyzes the Ucp1-CreEvdr transgenic mouse line and finds it causes complex developmental and metabolic issues independent of its intended genetic manipulation.

Chromatin Reprogramming of In Vitro Fertilized and Somatic Cell Nuclear Transfer Bovine Embryos During Embryonic Genome Activation

The paper investigates chromatin reprogramming during embryonic genome activation in bovine embryos, finding that SCNT embryos fail to reprogram certain EGA gene targets and that DUXC over-expression can rescue this defect.

Complete blood count and selected serum parameters of Venus transgenic rabbits

This study measures hematology and serum parameters in Venus transgenic rabbits to assess the impact of the fluorescent protein on hematopoiesis and general health.

Strategy for selective acquisition of transgenic marmosets using the piggyBac transposon system

The paper develops a method to identify transgenic marmoset embryos using a fluorescent protein indicator system based on the piggyBac transposon.

Germline Transmission of a Circular Human Artificial Chromosome in the Mouse

This study demonstrates that a circular human artificial chromosome can be transmitted through three generations of mice without health or reproductive issues.

Incomplete activation of developmentally required genes Alyref1 and Gabpb1 leads to preimplantation arrest in cloned mouse embryos

This study identifies Alyref and Gabpb1 as developmentally required genes whose incomplete activation causes preimplantation arrest in cloned mouse embryos.

A protocol for locating and counting transgenic sequences from laboratory animals using a map-then-capture (MapCap) sequencing workflow: procedure and application of results.

The paper presents a MapCap sequencing workflow to locate and count transgenic sequences in mice, identifying insertion sites and characterizing genetic variations in a diabetes model.

Group size planning of breedings of gene-modified animals

The paper develops an R package and website to optimize breeding design for gene-modified animals, minimizing animal use while ensuring successful outcomes.

Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish

This study successfully transplants spermatogonia between marine fish species in the Pleuronectiformes order, achieving high efficiency and producing donor-derived offspring.

Derivation and characterization of chimera-competent eXtra-Embryonic eNdoderm 1 (XEN) cells from pig blastocysts

The paper derives and characterizes XEN cells from pig embryos, showing their ability to contribute to chimerism and serve as nuclear donors for cloning.

Generation of transgenic quails by in vivo transfection of primordial germ cells

This paper generates transgenic quails using plasmid-based in vivo transfection of primordial germ cells, overcoming limitations of previous viral methods.

Flexible, fast and selective genetic manipulation of the vertebrate CNS with misPiggy

The paper introduces a transposon-based system for genetic manipulation of the vertebrate CNS, enabling long-term conditional experiments across cell types and species.

A transgenic pig model expressing a ZsGreen1 reporter across an extensive array of tissues

This study quantifies CMV promoter activity in various porcine tissues using a transgenic pig model expressing ZsGreen1, revealing tissue-specific expression patterns.

Effective Cell Immunoablation in Undisrupted Developing Avian Embryos

The paper describes an immunosurgery method to effectively ablate endogenous primordial germ cells in chick embryos, enhancing chimerism rates when grafting foreign PGCs.