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Transcriptomic, Specific Marker, and Pathway Analysis of Smooth Muscle Cell Foam Cells Relative to Macrophage Foam Cells in Human Atherosclerosis

Smooth muscle foam cells in early-to-intermediate human coronary plaques map to fibromyocytes, express CFH and SERPINE1 unlike macrophage foam cells, and show matrix, coagulation and angiogenesis programs.

A complete diploid human genome benchmark for personalized genomics

T2T-HG002v1.1, a complete diploid HG002 assembly lacking detectable errors over 99.4% of its length, enables genome-based benchmarking of assemblies, sequencing reads, and phased variant calls.

Stepwise DNA unwinding gates TnpB genome-editing activity

Ymu1 TnpB unwinds DNA via a long-lived partially unwound intermediate before a rarely stable open state; the WFR triple variant stabilizes both, boosting plant editing.

Modeling the structure-conditioned sequence landscape for large-scale protein design with TriFlow

TriFlow, discrete flow-matching sequence designer with global three-track structure attention, raises in silico binder success over ProteinMPNN in RFdiffusion and BindCraft-style pipelines on 500 targets and flags functional sites.

From sound to source: Human and model recognition of environmental sounds

Neural networks trained to detect sound categories in synthetic multi-source scenes approach human accuracy on the EnvAudioEval behavioral benchmark and track human patterns better than cochlear or cortical filterbank baselines.

Impaired astrocyte-to-neuron cholesterol trafficking drives synaptic dysfunction in Rett syndrome

Mecp2 knock-out mouse astrocytes accumulate cholesterol yet release poorly lipidated ApoE; cholesterol supplementation restores synaptic puncta density in neurons exposed to their conditioned medium and in Mecp2 heterozygous neurons.

Multimodal Imaging Reveals Spatial Host-Pathogen Microenvironments in Escherichia coli Meningoencephalitis

Rat E. coli meningoencephalitis maps into ventricular, meningeal and perivascular niches: biofilm-like aggregates, aerobactin and salmochelin signals, calprotectin, neutrophil peptides, reduced proenkephalin peptides, with some markers recoverable in CSF.

MDA5 multimerization on LINE RNA drives pathogenic extracellular immune complexes in autoimmunity

Patient-derived anti-MDA5 antibodies bind MDA5 filaments in two germline-encoded modes; only filament immune complexes activate innate immunity, with LINE-derived dsRNA scaffolding circulating filaments in patient plasma.

The Human Bindome: A Proteome-scale Atlas of Designed Binder Candidates

A parallelized BindCraft pipeline generated 306,146 in silico binder candidates against 8,296 human proteins, each with sequence, predicted complex and confidence metrics, freely accessible online.

Epigenetic and brain age across development: Performance and associations in the MIND consortium

Across 15 cohorts (birth to 24 years), 20 epigenetic clocks and eight brain age models predicted chronological age modestly, stabilizing by middle childhood; their age residuals were weakly associated.

H3K27 trimethylation maintains baseline network excitability in epilepsy

In mice 4 days after kainate-induced status epilepticus, H3K27me3 gains coincide with repression of excitability genes; EZH2 inhibition with UNC1999 lowers seizure threshold acutely and increases chronic seizure frequency.

The linker histone H1.4 condenses chromatin in maturing postmitotic neurons

Epitope-tagged knockin mice show histone H1.4 protein accumulates in postmitotic neurons, shifts toward B-compartment heterochromatin with maturation, and H1f2/H1f4 loss impairs cerebellar granule neuron chromatin compaction.

A repressive regulatory cascade shapes temporal patterning of activity-regulated gene expression in a defined sensory neuron type

TRAP-seq of C. elegans AFD neurons reveals three temperature-duration expression modules; CREB-driven RCAN-1 acts in parallel with MEF-2 to repress premature expression of a delayed gene, ins-39.

Structural Mechanisms of DNAJC13 Dimeric Assembly and InsP6 binding in Recycling Endosome Regulation

Cryo-EM at 2.4 Å shows DNAJC13 as an antiparallel arch-shaped homodimer with a buried InsP6 pocket; dimerization controls WASH recruitment, tubule fission, and melanin production.

BioPathfinder: Evidence-guided multi-agent platform enables hypothesis discovery for CAR-T engineering

A provenance-tracked multi-agent system curated 57 CAR-T patient scRNA-seq studies, generated reviewable hypotheses, and prioritized KLRC1/NKG2A, whose blockade improved CD8+ CAR-T antitumour activity in a Raji xenograft model.

Bio-CM{superscript 2}: Distributed computational optics for cortex-widecellular imaging

Four miniature lenses sharing one CMOS sensor, with computational stitching, give cellular-resolution imaging over 7.5 × 10 mm^2 at video rate, resolving 3,474 mouse cortical neurons.

Comparison of multiple video tracking-based behavioral summary approaches for compound discrimination

Scalar keypoint aggregates matched Keypoint-MoSeq, VAME, SimBA and A-SOiD at discriminating eight drug-dose treatments across 314 mouse open-field recordings; social context did not improve recall.

Circulating extracellular vesicles in plasma carry accessible molecular signatures of aging in mice

Mass spectrometry of Mag-Net-enriched plasma extracellular vesicles from 86 C57BL/6J mice reveals 272 age-correlated proteins, sex-divergent trajectories, and an age clock with 2.75-month mean absolute error.

Preformed Fibril Seeding Reshapes the Phosphorylated α-Synuclein Proximal Proteome in the Olfactory Bulb

Proximity proteomics in PFF-seeded mouse olfactory bulb shows aggregated phospho-S129 alpha-synuclein mostly reprioritizes existing interactions toward axon-glia, myelin and proteostasis proteins, with a conserved 107-protein signature.

Novel human monoclonal antibodies with enhanced sensitivity for lipoarabinomannan antigens present in urines of TB patients

Converting human antibody A194-01 from IgG1 to IgM broadens glycan recognition and boosts urinary lipoarabinomannan detection, and RU95-C1, a mannan-targeting antibody, works as a capture partner.