flow cytometry

Proteasome inhibition enhances latency reversal and boosts NK cell-mediated elimination of HIV-1 infected cells through HLA-E downregulation

This study shows proteasome inhibition reverses HIV-1 latency and enhances NK cell killing by reducing HLA-E expression.

Influenza A virus membrane fusion is regulated by the balance between receptor binding and cleavage

This study shows HA-receptor interactions regulate influenza A virus membrane fusion, with receptor density, HA affinity, and NA activity affecting fusion efficiency.

Ocrelizumab Modulates Both B and T Cell Immune Capacities in Multiple Sclerosis

This study examines how ocrelizumab affects B and T cell populations in MS patients, revealing changes in B cell subsets and T cell dynamics, along with altered signaling pathways.

Integrated evaluation of immune system perturbation using structural, functional and cellular immunotoxicity endpoints in rats

CD5L insufficiency exacerbates skeletal joint damage in rheumatoid arthritis

This study shows CD5L deficiency worsens joint damage in RA by promoting inflammation and non-classical monocyte expansion.

Safety and Feasibility of Infusing Ex Vivo Expanded Allogeneic Canine Natural Killer Cells for the Treatment of Metastatic Solid Tumors

This study expands and activates allogeneic canine NK cells ex vivo, demonstrating their safety and in vitro potency for treating metastatic solid tumors.

Natural killer cell dysfunction drives keloid pathogenesis

This study identifies NK cell dysfunction in keloids, showing IFN-γ suppresses fibroblast proliferation while AREG counteracts this, and systemic IFN-β drives NK cell exhaustion.

Transient immune landscape remodelling shapes CD8 T-cell priming during infection

The paper shows that transient immune architecture changes during infection protect low-affinity CD8 T-cell priming from Treg inhibition, ensuring broad T-cell responses.

Dengue infection elicits skin tissue-resident and circulating CD8+ T-cells associated with protection from hospitalization.

Flow cytometry-based evaluation of hepatic infection by non-fluorescent Plasmodium parasites

This paper introduces a flow cytometry-based method to quantify hepatic infection by non-fluorescent Plasmodium parasites using HSP70 and UIS4 as markers.

The bone marrow microenvironment of RAS pathway mutant B-ALL is enriched for immunosuppressive regulatory T cells

This study identifies an immunosuppressive bone marrow microenvironment in RAS-mutant B-ALL, enriched for regulatory T cells, and suggests CTLA-4 blockade could enhance T-cell-engager efficacy.

Single Spectral Flow Cytometry Panel for Simultaneous Detection of Hematopoietic Stem, Progenitor, and Mature Lineages in Mouse Bone Marrow

The paper introduces a 19-marker spectral flow cytometry panel to simultaneously identify hematopoietic stem, progenitor, and mature cell lineages in mouse bone marrow.

EpiFlow: multidimensional single-cell epigenetic profiling by spectral flow cytometry

EpiFlow quantifies 16 epigenetic markers per cell using spectral flow cytometry, showing broad applicability across species and biological processes.

Age determines NK cell fate and tissue compartmentalization to CMV infection

The paper shows that NK cell responses to CMV infection differ between infants and adults, influenced by age and T cell immunity, with infant NK cells exhibiting adaptive-like features and tissue seeding.

Addition of DMSO to T cell cultures skews differentiation towards a memory phenotype.

The paper shows that low DMSO concentrations skew T cell differentiation towards a memory phenotype during in vitro expansion.

Knockdown of the long isoform of the prolactin receptor selectively targets pathogenic immune cells and averts lupus nephritis

This study shows that knocking down the long isoform of the prolactin receptor reduces pathogenic immune cells and prevents lupus nephritis in SLE-prone mice.

Alternatively activated monocyte-derived myeloid cells promote extracellular pathogen persistence within pulmonary fungal granulomas.

This study identifies alternatively activated myeloid cells as drivers of extracellular fungal persistence in pulmonary granulomas through T helper 2 cell signaling.

Addressing complex autofluorescence signatures in solid tissue samples to enhance full spectrum flow cytometry of non-immune cells.

This paper optimizes full spectrum flow cytometry for analyzing non-immune cells in solid tissues by addressing autofluorescence and improving subpopulation resolution.

ILC2s navigate tissue redistribution during infection using stage-specific S1P receptors

This study shows that ILC2s use different S1P receptors at distinct stages to migrate during infection, highlighting a shared vascular network for lymphocyte movement.

DropletFactory CORE - a droplet cytometry and sorting platform for fast and accessible screening in biotechnology

The paper introduces a droplet cytometry and sorting platform for fast and accessible screening of yeast cells based on fluorescence signals.

Mineralized collagen scaffold pore architecture and glycosaminoglycan content biases anti-inflammatory macrophage phenotype

This study shows how scaffold pore size, alignment, and glycosaminoglycan content influence macrophage polarization toward M2-like phenotypes in 3D mineralized collagen scaffolds.

A cell surface proteomic atlas reveals socioeconomic status associated immune diversity

This study maps SES-related immune diversity using flow cytometry, finding lower SES associates with downregulated surface proteins and upregulated immune checkpoints.

The membrane-associated ubiquitin ligase MARCHF8 promotes cancer immune evasion by degrading MHC class I proteins

This study identifies MARCHF8 as a driver of cancer immune evasion by degrading MHC-I proteins and shows its inhibition enhances antitumor immunity and response to immunotherapy.

HIF-1α+ CD4 T cells coordinate a tissue resident immune cell network in the lung

This study identifies HIF-1α+ CD4 T cells as key coordinators of a tissue-resident immune network in the lung during infection and cancer.

A multidisciplinary approach for the study of age-dependent expression patterns in porcine experimental wounds

This study uses a multidisciplinary approach to identify age-dependent expression patterns in porcine experimental wounds to improve objective wound age estimation in forensic cases.

Evaluating 6- and 18-hour stimulation durations for natural killer cell degranulation (CD107a assay) to optimize workflow efficiency in a clinical immunology laboratory

This study compares 6-hour and 18-hour stimulation durations for NK cell degranulation assays and finds the 6-hour protocol is more reliable and efficient.

Rifampicin-induced Staphylococcus aureus persister formation is driven by CodY regulon and oxidative stress level

This study identifies CodY regulon and oxidative stress as drivers of rifampicin-induced S. aureus persister formation using single-cell analysis.

High-Throughput Single-Cell Spectroscopy Using Phasor Analysis of Spectral Flow Cytometry

This paper introduces phSFC, a method using spectral flow cytometry and phasor analysis to analyze membrane properties in single cells, showing it matches hyperspectral microscopy results.

L1CAM-CAR T cells with enhanced potency overcome low-density antigen expression in rhabdomyosarcoma

This study develops L1CAM-CAR T cells that effectively target rhabdomyosarcoma despite low antigen density, showing improved efficacy and safety compared to existing CARs.

Antibody responses against bacterial glycans affinity mature and diversify in germinal centers.

The paper shows that glycan-specific B cells undergo class-switching and diversification in germinal centers with age and infection, influencing vaccine design.