flow cytometry

Characterization of a heterogenous activated B cell compartment arising early after antigen exposure preceding long-lived memory B cell formation

The paper characterizes early antigen-specific B cell responses after vaccination, identifying distinct clusters with different contraction dynamics and potential roles in memory B cell formation.

An innovative in vitro model for studying the biology of cardiac fibroblasts originating from the epicardium

This study develops an in vitro mouse model to study epicardium-derived cardiac fibroblasts using lineage tracing and immortalized cell cultures.

Superior intracellular detection of cytokines, transcription factors, and phosphoproteins by CyTOF compared with fluorescent cytometry

This study compares CyTOF with fluorescent cytometry for detecting intracellular markers, finding CyTOF provides superior resolution for phosphoproteins, transcription factors, and cytokines, enabling better immune cell clustering and discovery of unique populations.

Adult mice with neonatal-like T cell subsets exhibit increased susceptibility to Bordetella pertussis and influenza infection

This study shows that adult mice with neonatal-like T cell subsets are more susceptible to Bordetella pertussis but not influenza, highlighting the role of thymic development in immune responses.

Tumors Located in the Brain Impair the Frequency and Phenotype of Dendritic Cells in Blood and Tumor

This paper identifies systemic reductions in dendritic cell (DC) numbers and activation markers in brain tumor patients, with brain tumors directly contributing to DC defects, and highlights corticosteroid use as a confounding factor.

AD-HIES patients retain preTh17-cells that produce IL10 with opportunistic pathogens and induce IgE

The paper identifies IL-10-producing pre-Th17 cells in AD-HIES patients that respond to pathogens and support IgE production despite STAT3 mutations.

Longitudinal spatial profiling of neutrophils during adoptive T cell therapy in murine melanoma reveals distinct lymph node infiltration patterns across anatomical sites

This study examines neutrophil infiltration patterns in lymph nodes during adoptive T cell therapy in mice, revealing differences between tumor-draining and non-tumor-draining lymph nodes.

Comparison of CD4 T cell response in Plasmodium falciparum and vivax malaria

This study compares CD4 T cell responses to Plasmodium falciparum and P. vivax malaria, finding similar activation and proliferation but species-specific differences in peripheral Tfh cell subsets and IL-10 production.

Identification of a Highly Functional Effector CD8+ T Cell Program After Transplantation in Mice and Humans

This study identifies a functional effector CD8+ T cell program in mice and humans that persists into memory after antigen clearance, driven by CD43 1B11 expression.

Immunometabolic determinants of long-term response in leukemia patients receiving CD19 CAR T cell therapy

This study identifies immunometabolic factors in CD19 CAR T cells that predict long-term response in B-ALL patients and suggests mTOR inhibition as a strategy to enhance anti-tumor activity.

Analysis of microalgae autofluorescence using full-spectrum cytometry to discriminate and monitor microalgae and bacteria

The paper uses full-spectrum cytometry to analyze microalgae and bacteria by their autofluorescence, revealing taxonomic differences and interactions affecting pigment signatures.

Characterization of nanoparticles and fluorescent recombinant extracellular vesicles with three high-sensitivity flow cytometers

This study compares three high-sensitivity flow cytometers for analyzing nanoparticles and fluorescent extracellular vesicles using controlled conditions and different detection methods.

Four Core Genotypes Mice Exhibit Quantitative Differences in T and B Cell Subpopulations compared to Wild-type Mice.

The paper compares T and B cell subpopulations in Four Core Genotypes mice to wild-type mice, finding sex chromosome effects on immune cell populations.

Mechano-activation of synovial fibroblasts and macrophages during OA progression in the dynamically stiffening synovial microenvironment

This study investigates how synovial microenvironment stiffness changes during OA progression and how it affects fibroblast and macrophage behavior through mechanosignaling and cell-cell interactions.

CaptureBody - an anti-CD45 x anti-IgG bispecific antibody enables accurate unmixing for spectral flow cytometry

The paper introduces CaptureBody, a bispecific antibody for spectral flow cytometry that improves unmixing accuracy by using cells instead of compensation particles.

Elevated ferritin as a companion biomarker of myeloid-driven inflammation in RNP/Sm co-positive, treatment-resistant SLE endotype

This paper identifies an RNP/Sm co-positive SLE endotype with myeloid-driven inflammation, hyperferritinaemia, and treatment resistance, suggesting targeted therapies are needed.

Glucose-Responsive CD20+ Cytotoxic T Cells: A Novel Pro-inflammatory Mediator in the Immunopathogenesis of Type 2 Diabetes

This study identifies CD20+ cytotoxic T cells as glucose-responsive, hyperfunctional cells in T2D that correlate with metabolic parameters and may contribute to inflammation.

Oxidative Stress-Induced Immunogenic Cell Death Enhances Whole-Cell Vaccine Efficacy in a Syngeneic Pancreatic Cancer Model

This study shows that inducing oxidative stress in pancreatic cancer cells creates an effective whole-cell vaccine that enhances anti-tumor immunity and synergizes with PD-1 blockade.

Objective method to estimate the duration of the DNAreplication cycle without perturbation in Escherichia coli.

This paper introduces an automated method to estimate the DNA replication cycle duration in E. coli using flow cytometry data without requiring pharmacological treatments.

X-CODE: a dual RNA barcoding system for multi-platform clonal tracking and spatial phenotyping

X-CODE enables multi-platform clonal tracking and spatial phenotyping by combining RNA barcoding with sequencing and imaging technologies.

EBV Type 1 versus Type 2: A determinant of NK cell anti-tumor activity in Burkitt lymphoma

This study compares ADCC activity of CD56neg and CD56dim NK cells against EBV-type 1 and 2 Burkitt lymphoma cells, finding EBV-T2 cells are more susceptible to direct cytotoxicity.

Divergent phenotypic and functional roles of human T follicular helper cells from infancy to adulthood

This study examines age-related differences in T follicular helper cell function and their impact on antibody responses across human development.

Blinatumomab-driven T-cell activation in αβ and γδ T-cell subsets: Insights from in vitro assays

This study evaluates BLN-driven cytotoxicity in β and γδ T-cell subsets, finding β T cells show superior cytotoxicity while γδ T cells exhibit stable effector-memory states and reduced susceptibility to activation-induced cell death.

Systemic immune dysregulation in hypertensive disorders of pregnancy persists years after delivery

This study identifies persistent immune dysregulation in women with hypertensive disorders of pregnancy years after delivery, linked to increased cardiovascular disease risk.

Suppression of CD4 T cells by Tregs dictates metabolic remodeling of CD8 T cells during effector to memory transition

This study identifies Treg-mediated suppression of CD4 T cells and inflammatory myeloid cells as critical for metabolic remodeling of CD8 T cells during the effector-to-memory transition.

Duration of Initial Viremia Modulates Functional Properties of HIV-specific T Cell Receptors

This study examines how the duration of initial HIV-1 viremia affects the functional properties of HIV-specific T cell receptors in individuals with HLA-B*58:01.

Dengue virus-specific memory B cell subsets differ as a function of infection history

This study compares DENV-specific memory B cell subsets in primary and secondary dengue infections, finding qualitative differences in subset frequencies and timing of peak responses.

Organ-specific immune responses are strain-dependent in a mouse model of Cryptococcus neoformans brain infection

This study examines how different Cryptococcus neoformans strains affect organ-specific immune responses in a mouse model of cryptococcal meningitis, finding strain-dependent effects independent of virulence.

CD8+CD20+ Cytotoxic T Lymphocytes Exhibit Augmented Degranulation and Pro-inflammatory Potential in Multiple Sclerosis

This study identifies CD8+CD20+ cytotoxic T lymphocytes in MS patients, showing increased degranulation and pro-inflammatory cytokine production, particularly Granzyme K, contributing to CNS inflammation.

IL-21 selectively augments cytotoxic potential of antigen-activated MAIT cells

This study shows IL-21 enhances cytotoxicity of activated MAIT cells by increasing GrzB and perforin expression without significantly affecting cytokine production.