Pluripotent Stem Cells Research

This paper develops a co-culture method for human iPSC-derived astrocytes and motor neurons in MEA systems, showing bidirectional maturation effects.

This study develops a scalable monolayer culture system for generating erythroid and multilineage hematopoietic progenitors from human iPSCs, showing limited HSC engraftment potential.

This study investigates how cell cycle regulators influence mesoderm specification in human pluripotent stem cells, finding that CDKs and pRb phosphorylation are context-dependent for efficient mesoderm formation.

The paper shows that fluctuations in OCT4 and SOX2 levels influence cell fate decisions by affecting chromatin accessibility at differentiation enhancers.

The paper uses MINUTE-ChIP to analyze H3K27me3 and H3K4me3 in mouse ESCs under 2i and serum conditions, finding hypermethylation of H3K27me3 and loss of H3K4me3 at bivalent promoters.

This study generates implantation-competent blastocyst-like structures (iBCs) from mouse pluripotent stem cells, demonstrating their ability to implant and develop in vivo.

The paper develops Reprogram-Seq to screen TF cocktails for reprogramming cell states, identifying a 3-TF combination that reprograms MEFs to epicardial-like cells.

This study identifies ZIC3 as a key regulator of the transition from naïve to primed pluripotency in mouse ESCs by analyzing open chromatin changes and transcriptional networks.

The paper analyzes in vitro NMPs derived from ESCs and EpiSCs, finding that EpiSC-derived NMPs more closely resemble in vivo NMPs and contain a node-like population maintaining T expression.

The paper analyzes cell cycle dynamics in human pluripotent stem cells primed for differentiation using RNA-sequencing and identifies PI3K signaling's role in early differentiation states.

The paper shows that KLF4 protein stability, regulated by interactions with pluripotency factors, overrides transcriptional control in ES cells, and mutations affecting stability prevent differentiation.

The paper identifies RNA binding proteins and RNAs interacting with Sox2 in mouse embryonic stem cells, showing coexistence in a ribonucleoprotein complex without evidence of chromatin recruitment regulation.

The paper maps multi-omic changes during mouse pluripotency transition, showing phosphoproteome dynamics precede epigenome and transcriptome changes.

This study identifies reprogramming checkpoints in iPSC generation by analyzing single-cell transcriptomes and reveals key gene interactions and regulatory mechanisms.

The paper uses RNAi screens in isogenic human stem cells to identify SMARCA4 as a differential regulator of stemness and differentiation in hematopoietic and neural stem cells.

The paper compares hypoxia responses in human and chimpanzee iPSC-derived cardiomyocytes, finding conserved transcriptional responses and species-specific regulatory differences.

This study identifies DPPA2 and DPPA4 as essential for activating Dux and LINE-1 transcription, which is critical for zygotic genome activation in mouse embryos.

The paper identifies Brca1, Bard1, and Wdr5 as factors linking DNA damage response to MET during reprogramming, using siRNA screening and colony morphology analysis.

The paper uses single cell transcriptomics to show that transdifferentiation and reprogramming can be uncoupled in pre-B cells based on their intrinsic plasticity.

This study shows how Nanog uses different mechanisms to enhance embryonic stem cell self-renewal by rewiring pluripotency networks and blocking differentiation through histone modification.

This study investigates the dynamics of X-chromosome reactivation during mouse iPSC reprogramming, revealing hierarchical gene activation patterns and the role of chromatin regulators.

The paper analyzes pig embryos at single-cell resolution to study lineage segregation, pluripotency, and X-chromosome inactivation during early development, revealing conserved mechanisms with human embryos.

This study identifies Dppa2 and Dppa4 as upstream regulators of the Dux-driven zygotic transcriptional programme in 2C-like cells.

The paper develops a standardized protocol for accurately identifying cardiomyocytes using flow cytometry by evaluating anti-troponin antibodies and sample preparation methods.

This study shows that X-chromosome dosage in mouse iPSCs affects chromatin accessibility, transcriptome, growth, and pluripotency exit, largely independent of DNA methylation.

This study demonstrates automated control of pluripotency in mouse embryonic stem cells using microfluidics and feedback algorithms to regulate Rex1 expression.

The paper shows that TEAD4/YAP1/WWTR1 repress Sox2 expression before the 16-cell stage in mouse embryos, preventing premature pluripotency.

The paper identifies a multipotent progenitor population derived from Epiblast Stem Cells that self-renew and express a gene signature similar to embryonic NMPs.

The paper reports the generation of four iPSC lines from PBMCs of an ADHD patient and their healthy sibling, confirming pluripotency and differentiation potential.

This study validates the differentiation of rhesus macaque primordial germ cell-like cells (rPGCLCs) using transplantation into adult gonadal niches, showing they commit to late PGCs and spermatogonia-like cells but not ENO2-positive spermatogonia.