Advanced Fluorescence Microscopy Techniques

Smart hybrid microscopy for cell-friendlydetection of rare events

This paper introduces hybrid-EDA, a microscopy framework that reduces phototoxicity while capturing rare biological events using event-driven fluorescence acquisitions.

Ultrashort optical-pin excitation for scattering brain imaging

This paper introduces an optical pin excitation technique to improve 3D imaging in scattering brain tissue, achieving enhanced contrast and robustness to scattering.

Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics

This paper introduces tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric brain imaging, demonstrating its use in mapping cerebral blood flow, neurovascular coupling, and neuroimmune responses.

Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy

This paper introduces Cy-ExM, a high-plex imaging strategy that enables 3D super-resolution mapping of cellular ultrastructure in mammalian cells.

Real-Time Sensing of Food Spoilage Using a Recombinant mVenus-Tolles FRET pH Biosensor

This paper evaluates a FRET-based biosensor for real-time pH monitoring as an indicator of food spoilage, showing pH-responsive signals in both buffer solutions and chickpea paste.

Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM

Benchmarking fluorescence lifetime estimators for the membrane mechanosensor Flipper-TR across microscopes, laser frequencies, and photon budgets establishes practical guidelines for quantifying relative lipid packing dynamics in live cells.

A Unified and Interpretable Framework for Evaluating Fluorescence Trace Quality in Transcription Kinetics

This paper develops a framework to evaluate fluorescence trace quality for transcription kinetics by identifying thresholds for signal-to-noise ratio and trace length using synthetic data.

Lipid-Coated Water-in-Oil Droplets as a Passivation-Free Platform for Cost-Effective Fluorescence Spectroscopy

This paper introduces a passivation-free platform using lipid-coated water-in-oil droplets for fluorescence spectroscopy, enabling accurate measurements of molecular dynamics in nanolitre volumes.

Simultaneous Label-free Imaging of Nucleolar Dynamics and Subcellular Metabolic Shifts Across Tissue Contexts

This paper develops a label-free imaging platform to study nucleolar dynamics and metabolic shifts in tissues, revealing NADH depletion under nucleolar stress and progesterone-induced changes in endometrial organoids.

A systematic approach for the purification of fluorophore-labelled proteins via anion exchange chromatography

The paper presents a systematic method for purifying fluorophore-labeled proteins using anion exchange chromatography, achieving high yields for FRET studies.

Refractive index mapping below the diffraction limit via single molecule localization microscopy

This paper introduces a method combining SMLM and AFM to map refractive index at sub-diffraction resolution, revealing structural heterogeneity in collagen fibrils.

Residual blinking-driven channel alignment for multicolor single-molecule localization microscopy

This paper introduces ReBling alignment, a method for channel alignment in multicolor single-molecule localization microscopy that uses residual blinking events to achieve accurate registration without fiducials.

Two-colour Imaging Fluorescence Correlation Spectroscopy (ImFCS) probes the influence of the juxta-membrane actin-cortex

The paper develops a two-colour ImFCS method to quantify how the juxta-membrane actin cortex influences plasma membrane dynamics by measuring reporter diffusion.

Rapid fluorescence lifetime sensor development of LifeCamp enables transient and baseline absolute calcium measurements

This paper introduces LifeCamp, a high-speed calcium lifetime sensor developed using RALISED, enabling accurate measurement of baseline and transient calcium levels in neurons.

In vivo Calcium Imaging in the Near-Infrared II Window

This paper develops a near-infrared II calcium imaging technique using a bacterial protein to enable high-sensitivity, non-invasive deep-tissue calcium imaging in live mammals.

Mechanical de-skewing enables high-resolution imaging of thin tissue slices with a mesoSPIM light-sheet microscope

The paper introduces a no-shear imaging protocol for mesoSPIM light-sheet microscopy to improve imaging of thin tissue slices with reduced processing time and better axial resolution.

Glutamate indicators with increased sensitivity and tailored deactivation rates

The paper develops two variants of a glutamate indicator with enhanced sensitivity and tailored deactivation rates for studying synaptic transmission in vivo.

Transient binding facilitates super-resolution imaging of functional amyloid fibrils on living bacteria

This study uses transient amyloid binding with Nile blue to enable super-resolution imaging of curli fibrils on living E. coli cells, providing structural insights into their assembly and function.

Structure, function and dynamics of mCoral, a pH responsive engineered variant of the mCherry fluorescent protein with improved hydrogen peroxide tolerance.

The paper describes the engineering of mCoral, a modified mCherry variant with improved hydrogen peroxide tolerance and pH responsiveness, and elucidates its structural and dynamic properties.

Outcome-Driven Microscopy: Closed-Loop Optogenetic Control of Cell Biology

This paper introduces outcome-driven microscopy, combining optogenetics and smart microscopy to achieve precise control over cellular behavior through predefined outcomes.

Assessing Knowledge Distillation of a Multi-Emitter Localizing Neural Network for Applications in Stochastic Optical Reconstruction Microscopy

This study evaluates knowledge distillation from a large SRM model to a smaller one, finding limited success in transferring multi-emitter localization performance.

Optimizing effective labeling efficiency in MINFLUX 3D DNA-PAINT microscopy by maximizing marker detection probability.

The paper investigates optimizing labeling efficiency in MINFLUX 3D DNA-PAINT microscopy by improving marker detection probability through repeat DNA-PAINT and adjusting acquisition parameters.

Real-Time Wide-Field Fluorescence Lifetime Imaging via Single-Snapshot Acquisition for Biomedical Applications

This paper introduces a real-time fluorescence lifetime imaging method using single-snapshot acquisitions, demonstrating its application in biomedical scenarios from microscopic to macroscale.

A simple, ultrastable, and cost-effective oxygen-scavenging system for long-term DNA-PAINT imaging

This paper introduces an enzyme-free oxygen-scavenging buffer for DNA-PAINT imaging that improves stability, reduces costs, and extends imaging duration.

Single-molecule FRET-tracking of InlB-activated MET receptors in living cells

The paper uses single-molecule FRET and tracking to study MET receptor activation by InlB in living cells, finding a dimeric complex lifetime of ~1 second and slower diffusion for dimers.

Angström Resolution with Flow Immunofluorescence Localization Microscopy (FILM)

This paper introduces FILM, a single-molecule localization microscopy technique achieving angstrom-scale resolution using flow-based imaging and standard fluorescent probes.

Landscape Expansion Microscopy Reveals Interactions between Membrane and Phase-Separated Organelles

The paper introduces land-ExM, a super-resolution microscopy technique that enhances visualization of membrane organelles and phase-separated structures, revealing their 3D interactions.

Observing Grazing Behavior Transitions in Cafeteria roenbergensis with Video-Rate Two-Photon Microscopy

This paper uses video-rate two-photon microscopy to observe and quantify grazing behavior transitions in Cafeteria roenbergensis, revealing phase-specific durations and physiological state-dependent dynamics.

Enhancing STED Microscopy via Fluorescence Lifetime Unmixing and Filtering in Two-Species SPLIT-STED

This paper introduces a method to improve STED microscopy by using fluorescence lifetime unmixing and filtering to enhance resolution and accuracy in two-species imaging.

Spatial Polarization-Induced Fluorescence Fluctuation Imaging (SPIFFI) Enables Single-shot Super-Resolution and Multidimensional Imaging

SPIFFI enables single-shot super-resolution and six-dimensional imaging by leveraging polarization-induced fluorescence fluctuations, enhancing live-cell imaging capabilities.