Advanced Fluorescence Microscopy Techniques

In vivo aberration measurement and correction for ultrafast FACED two-photon fluorescence microscopy of the brain

This paper develops an adaptive optics method to correct optical aberrations in ultrafast two-photon microscopy, improving resolution and signal in live mouse brain imaging.

In vivo autofluorescence lifetime imaging of spatial metabolic heterogeneities and learning-induced changesin the Drosophila mushroom body

This study uses NAD(P)H FLIM to map metabolic profiles in Drosophila mushroom bodies, revealing spatial heterogeneities and learning-induced metabolic changes.

Sensitive and Selective Next-Generation FRET-based PKA Biosensors

The paper introduces AKAR6, a new suite of FRET-based PKA sensors with enhanced sensitivity and selectivity for detecting compartment-specific PKA activity in live cells.

Highly Sensitive Chemigenetic FRET-Based Kinase Biosensors

This paper develops highly sensitive chemigenetic FRET-based biosensors for kinase and signaling activity with improved dynamic range and spectral properties.

Reaction-enabled, highly sensitive super-resolution imaging

This paper introduces RIED, a super-resolution imaging method using electrochemiluminescence, achieving high sensitivity and resolution for intracellular structures.

Extracting large-scale neural activity with Suite2p

Suite2p processes large-scale neural recordings, outperforming existing methods in motion correction and cell detection.

Monitoring intracellular antibiotic concentrations in real-time using allosteric biosensors

The paper develops genetically-encoded biosensors to monitor antibiotic concentrations inside bacterial cells and tissues in real-time, revealing antibiotic exposure dynamics and penetration in infection models.

Altair-LSFM: A High-Resolution, Easy-to-Build Light-Sheet Microscope for Sub-Cellular Imaging

Altair-LSFM is a high-resolution, open-source light-sheet microscope designed for sub-cellular imaging, achieving 235 nm lateral and 350 nm axial resolution.

Single-molecule localization microscopy reveals the molecular organization of endogenous membrane receptors

The paper develops an optimized immunolabeling protocol to quantify endogenous membrane receptor stoichiometry using single-molecule localization microscopy on Jurkat T cells and primary multiple myeloma cells.

Visualizing PINK1 Activity Dynamics in Single Cells with a Phase Separation-Based Kinase Activity Reporter

This paper develops a phase separation-based PINK1 biosensor to visualize real-time PINK1 activity in single live cells and study its role in mitophagy.

Caging-group-free photoactivatable fluorophores with far-red emission

This paper introduces caging-group-free photoactivatable fluorophores with far-red emission for improved super-resolution microscopy.

An mTurquoise2-based glucose biosensor

The paper develops a glucose biosensor, TINGL, for measuring intracellular glucose dynamics in yeast and human cells, showing it detects glucose concentrations up to 1 mM and responds to glucose addition in starved cells.

FRAPPe - a novel FRET-based biosensor to detect inorganic polyphosphates

The paper introduces FRAPPe, a FRET-based biosensor for detecting inorganic polyphosphates, which can quantify polyP levels and is useful for high-throughput screens.

Combinatorial DNA-PAINT

Combi-PAINT improves multiplexing in super-resolution microscopy by using combinatorial DNA barcoding, resolving 41 targets with high accuracy in under 30 minutes.

Analyzing single-molecule dynamics with both complex types of motion and complex transition kinetics: Benchmarking of ExaTrack

This paper benchmarks the ExaTrack tool for analyzing single-molecule dynamics, focusing on its ability to handle complex motion types and transition kinetics using simulated data.

Super-resolved imaging of mRNA ultrastructure in cells

The paper introduces Combi-PAINT, a method for super-resolved mRNA imaging, revealing mRNA ultrastructures and molecular patterns in human cells.

Label-Free Monitoring of Cancer-associated Fibroblast Activation using NADH Fluorescence Lifetime Imaging

This study uses NADH fluorescence lifetime imaging to monitor dynamic changes in cancer-associated fibroblast activation in real-time without labels.

Dual-color expansion microscopy of membrane proteins using bioorthogonal labelling

This paper develops dual-color expansion microscopy using non-canonical amino acid labeling to achieve high-resolution imaging of membrane proteins in HEK 293T cells.

High Throughput Hyperspectral and Multiplexed Super-Resolution Fluorescence Imaging by SP-STORM

This paper introduces SP-STORM, a method combining single molecule photoswitching and spectral phasor analysis to achieve high-throughput, multiplexed super-resolution imaging of subcellular structures.

FPmotion an Automated Signal Processing and Statistical Analysis Tool for Fiber Photometry Data

FPmotion automates preprocessing, integration, and statistical analysis of fiber photometry data, improving accessibility and reducing analysis time.

Quantitative Expansion Microscopy for In Situ Estimation of Endogenous Target Abundance

This paper introduces qExM, a method to estimate endogenous protein abundance in expanded samples, achieving a 9.4% mean percent error in nuclear pore complex subunit stoichiometry.

Deep learning-based event classification of mass photometry data for optimal mass measurement at the single-molecule level

This paper introduces a deep learning method to classify mass photometry events, improving single-molecule mass measurement accuracy by reducing artifacts and enhancing resolving power.

Volumetric single-molecule tracking inside subcellular structures

This paper introduces a method combining single-molecule light-field microscopy with widefield Fourier light-field microscopy to improve volumetric subcellular imaging and molecular diffusion analysis.

Grading HER2 at the nanoscale in clinical tissue

This study uses super-resolution microscopy to analyze HER2 nanoclustering in breast cancer tissues, revealing grade-dependent patterns and reclassifying samples based on nanoscale features.

Label-free Tracking of Subcortical White Matter Degradation In Vivo Using Third Harmonic Generation Microscopy in a Mouse Model of Multiple Sclerosis

This study uses THG microscopy to track subcortical myelin degradation in a mouse MS model, finding increased intranodal distances and a new quantitative metric for early detection.

Recombinant Photo-tagging Enables Fluorescent Labelling of Biomolecules and Visualization of Liquid-Liquid Phase Separation

This paper introduces a method to fluorescently label biomolecules using a photocleavable protein, enabling visualization of liquid-liquid phase separation with minimal perturbation.

Versatile high-speed volumetric imaging from microscopic to macroscopic scale by self-adaptive oblique plane microscopy

This paper introduces a self-adaptive oblique plane microscopy technique that enables high-speed volumetric imaging across microscopic to macroscopic scales in multi-cellular systems.

A one-step protocol to generate impermeable fluorescent HaloTag substrates for in situ live cell application and super-resolution imaging

The paper introduces a one-step method to create cell-impermeable fluorescent HaloTag substrates for live-cell imaging and super-resolution microscopy.

A flexible framework for automated STED super-resolution microscopy

The paper introduces autoSTED, a framework for automated STED microscopy that enables faster and more unbiased imaging of subcellular structures.

A plug-and-play ROI imaging module and deep-learning denoising framework extend three-photon microscopy to 1.7 mm depth

This paper introduces a hardware and software solution that extends three-photon microscopy depth to 1.7 mm by improving signal-to-noise ratio and noise removal.