Cellular transport and secretion

Dynamic regulation of the COPII interactome and collagen trafficking by site-specific glycosylation of Sec24D

The paper shows that O-GlcNAcylation of Sec24D regulates collagen trafficking by modulating its interactome and interaction with myoferlin in human cells and zebrafish.

ATP13A1 engages GET3 to facilitate substrate-specific translocation

The paper shows ATP13A1 uses GET3 to translocate cleaved signal sequences to the ER in a substrate-specific manner, ensuring efficient and accurate protein localization.

EndoNB: A general strategy to study the internalization of cell surface proteins

EndoNB enables analysis of surface protein turnover at endogenous levels using CRISPR/Cas9 and a cleavable probe, demonstrating its utility in studying internalization dynamics.

Ultrafast endocytosis in mouse cortical inhibitory synapses

The paper shows that inhibitory synapses in mouse cortical neurons have ultrafast endocytosis depots similar to excitatory synapses, suggesting similar endocytic mechanisms.

Membrane association prevents premature degradation and mitigates inefficient biogenesis of suboptimal membrane proteins

This study shows that membrane association stabilizes suboptimal membrane proteins like IAV M2, preventing premature degradation and enhancing biogenesis efficiency.

Functional redundancy in Oxa1-family insertases supports robust membrane insertion of influenza A virus M2 viroporin

This study shows that the influenza A virus M2 viroporin can use multiple membrane insertion pathways, including EMC and Oxa1-family translocons, ensuring robust viral infection.

Evolutionary origin and functional diversification of plant GBF1-type ARF guanine-nucleotide exchange factors

The paper investigates the evolutionary origin and functional diversification of plant GBF1-type ARF-GEFs, showing how AtGNOM and AtGNL1 orthologs evolved distinct roles in membrane trafficking.

Identification of ER:melanosome membrane contact sites in the retinal pigment epithelium.

The paper identifies ER:melanosome membrane contact sites in RPE and shows reduced ER contact with mature melanosomes, suggesting ER involvement in melanosome biogenesis and mitochondrial interaction.

EndoMAPV1, a Structural Protein Complex Landscape of Human Endosomes

The paper creates a structural interactome of human endosomes using cross-linking, mass spectrometry, and AlphaFold, identifying structural models for endosomal protein complexes.

Force-insensitive myosin-I enhances endocytosis robustness through actin network-scale collective ratcheting

The paper uses computational models to show that force-insensitive myosin-I enhances endocytosis robustness by reducing pit retraction, rather than through direct force transduction.

Dynamic Regulation of Vesicle Pools in a Detailed Spatial Model of the Complete Synaptic Vesicle Cycle

The paper models the synaptic vesicle cycle with high spatial and molecular detail, showing dynamic recycling and regulation by synapsin-1 and tomosyn-1 during sustained activity.

Dynamic early recruitment of GAK-Hsc70 regulates coated pit maturation

This study shows that GAK-Hsc70 early recruitment promotes CCP stabilization and curvature development during clathrin-mediated endocytosis.

The intracellular vacuolar pathogen Coxiella burnetii promotes exocytosis in a type IVB secretion system dependent manner

This study shows that Coxiella burnetii promotes exocytosis via its type IVB secretion system, leading to increased extracellular vesicle release and altered lysosomal protein trafficking.

Insulin-like peptide secretion is mediated by peroxisome-Golgi interplay

This study shows that peroxisomes interact with the Golgi in Drosophila insulin-producing cells to regulate sphingolipid composition and support insulin-like peptide secretion.

Vacuolar protein sorting-associated protein 1 (Vps1) has membrane constricting and severing abilities required for endosomal protein trafficking

This study identifies Vps1's membrane constricting and severing roles in endosomal trafficking, showing its requirement for fission and protein sorting in yeast.

Syntaxin 1A Transmembrane Domain Palmitoylation Induces a Fusogenic Conformation

This study uses molecular dynamics simulations to show that Stx1A TMD palmitoylation stabilizes its conformation, facilitates SNARE complex formation, and modulates fusion pore dynamics during neurotransmitter release.

PIKI-1, a class II phosphatidylinositol 3-kinase, functions in endocytic trafficking

This study identifies PIKI-1 as a Class II PI3K essential for PI(3,4)P2 production and early endosomal function in C. elegans, showing its role in suppressing NEKL-related molting defects.

Comprehensive Proteomic Characterization of Intra-Golgi Trafficking Intermediates

The paper characterizes intra-Golgi trafficking intermediates by analyzing proteomic profiles of Golgi-derived vesicles under COG complex dysfunction, revealing COG-dependent recycling of glycosylation machinery.

Flower/1 FLWR-1 regulates neuronal activity via the plasma membrane Ca2+-ATPase to promote recycling of synaptic vesicles

This study investigates the role of FLWR-1 in synaptic vesicle recycling and neuronal activity in C. elegans, finding it regulates Ca2+ levels via PMCA and affects excitation-inhibition balance.

Hemifusomes and Interacting Proteolipid Nanodroplets: Formation of a Novel Cellular Organelle Complex

The paper identifies a novel cellular organelle complex called hemifusomes through cryo-electron tomography, revealing their role in intraluminal vesicle biogenesis.

An unconventional interaction interface between the peroxisomal targeting factor Pex5 and Eci1 enables PTS1 independent import

The paper identifies an unconventional Pex5-Eci1 interaction interface that enables PTS1-independent peroxisomal import of Eci1 in yeast.

Spatial control of secretory vesicle targeting by the Ync13-Rga7-Rng10 complex during cytokinesis

The paper identifies a conserved complex that controls vesicle targeting during cytokinesis in fission yeast, ensuring proper cell division and integrity.

Sla2 is a core interaction hub for Clathrin Light Chain and the Pan1/End3/Sla1Complex

The paper identifies two CLC binding sites on Sla2 and shows Pan1 competes with CLC for a conserved site, revealing divergence in endocytic mechanisms between fungi and metazoa.

VPS35/Retromer-dependent MT1-MMP regulation confers melanoma metastasis

This study identifies VPS35/retromer as a regulator of MT1-MMP localization and stability, showing its role in melanoma metastasis and suggesting it as a therapeutic target.

RABEP1 amplifies front signaling in neutrophil migration

This study identifies RABEP1 as a key regulator of neutrophil migration by promoting endosomal recycling, Rac activation, and actin polymerization in zebrafish and human cells.

p125A (Sec23ip) couples COPII coat assembly with donor-acceptor membrane organization to facilitate tunnel-based traffic.

This study shows p125A stabilizes donor-acceptor membrane contact and promotes COPII outer layer assembly, facilitating tunnel-based collagen traffic.

Endothelial β-PIX (ARHGEF7) drives exocytosis through enabling the dynamic reorganisation of the cytoskeleton

This study identifies β-PIX as a regulator of VWF secretion from endothelial cells by enabling cytoskeletal reorganisation during WPB exocytosis.

Cytohesin-2 is essential for the survival of mice and regulates Golgi volume and function

This study shows that cytohesin-2 is essential for mouse survival and regulates Golgi volume and function through in vivo and in vitro analyses.

Endocytosis of a zinc transceptor ZIP4 is mediated by AP2 through an atypical dileucine motif

This paper identifies the LxL motif in ZIP4 as an atypical dileucine motif that mediates zinc-dependent endocytosis via AP2 and clathrin.

Cargo adaptors use a handhold mechanism to engage with myosin V for organelle transport

The paper shows that cargo adaptors bind myosin V through multiple sites, using a handhold mechanism to enhance motor-cargo interactions and regulation.