Heat shock proteins research

The heat shock transcription factor HSF-1 protects Caenorhabditis elegans from peroxide stress

This study shows HSF-1 protects C. elegans from oxidative stress through two distinct mechanisms depending on temperature conditions.

Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteins

This study investigates the functional interaction between the J-protein Tbj2 and cytosolic Hsp70/Hsp70.4 in Trypanosoma brucei, showing their cooperative role in protein refolding.

The HSV-1 immediate early protein ICP22 is a J-like protein required for Hsc70 reorganization during lytic infection

This paper shows that HSV-1's ICP22 protein functions as a viral J-like co-chaperone that interacts with Hsc70 to manage protein aggregation during lytic infection.

Cryo-EM structure of the ClpXP protein degradation machinery

This paper presents the first high-resolution cryo-EM structure of ClpXP from Listeria monocytogenes, revealing the binding interface and activation mechanism of the complex.

Peripherally expressed misfolded proteins remotely disrupt brain function and aggravate stroke-induced brain injury

This study shows that misfolded proteins in the heart can disrupt brain function and worsen stroke damage through prion-like mechanisms.

hsp-16.2 chaperone biomarkers track physiological states of proteome dosage

The paper identifies hsp-16.2 chaperone biomarkers as indicators of proteome dosage states in C. elegans, revealing cell-to-cell variation linked to gene expression differences.

Structural insights into the binding mechanism of Plasmodium falciparum exported Hsp40-Hsp70 chaperone pair

The paper presents structural analysis of the Plasmodium falciparum Hsp40-Hsp70 chaperone pair to elucidate their binding mechanism and inform drug design.

Remodeling of the C. elegans Non-coding RNA Transcriptome by Heat Shock

This study shows that heat shock in C. elegans affects the expression of various non-coding RNAs, some of which are directly regulated by HSF-1.

A ribosome assembly stress response regulates transcription to maintain proteome homeostasis

The paper shows that ribosome assembly stress triggers a pathway that balances Hsf1 and Ifh1 activity to prevent proteotoxic stress in yeast.

Distinct Structural Features of the Lon Protease Drive Conserved Hand-over-Hand Substrate Translocation

The paper determines the cryo-EM structure of Y. pestis Lon protease during substrate processing, revealing a distinct hand-over-hand translocation mechanism unique to the HCLR clade.

Over-expression of Hsp83 in grossly depleted hsrω lncRNA background causes synthetic lethality and l(2)gl phenocopy in Drosophila

The paper shows that over-expression of Hsp83 in the absence of hsrω lncRNAs causes synthetic lethality and phenocopies l(2)gl mutants in Drosophila.

The chaperone-client network subordinates cell-cycle entry to growth and stress

This study identifies chaperone systems as limiting factors for cell-cycle entry, showing their role in regulating nuclear accumulation of the G1 Cdk-cyclin complex and linking cell-cycle progression to growth and stress conditions.

Cholesterol bound Plasmodium falciparum co-chaperone ‘PFA0660w’ complexes with major virulence factor ‘PfEMP1’ via chaperone ‘PfHsp70-x’

The paper investigates the interaction between PFA0660w, PfHsp70-x, and PfEMP1 in Plasmodium falciparum, showing their role in PfEMP1 transport via cholesterol-rich J-dots.

Modulation of Human Hsp90α Conformational Dynamics by Allosteric Ligand Interaction at the C-Terminal Domain

The paper identifies three natural compound allosteric modulators of Hsp90 and assesses their effect on the protein's conformational dynamics.

Cellular responses to proteostasis perturbations reveal non-optimal feedback in chaperone networks

The paper investigates cellular responses to proteostasis impairments in yeast, finding that transcriptional responses often fail to restore proteostasis and that over-expression of alternative machineries can partially compensate for gene deletions.

Transgenic Zebrafish Models Reveal Distinct Molecular Mechanisms for Cataract-linked αA-Crystallin Mutants

This study uses transgenic zebrafish to compare molecular effects of cataract-linked αA-crystallin mutants, revealing differences in their impact on lens proteostasis.

Bacterial Hsp90 mediates the degradation of aggregation-prone Hsp70-Hsp40 substrates preferentially by HslUV proteolysis

The paper investigates the role of bacterial Hsp90 (HtpG) in collaboration with Hsp70 (DnaK) and shows that HtpG mediates degradation of aggregation-prone substrates via HslUV proteolysis.

It takes a dimer to tango: Oligomeric small heat-shock proteins dissociate to capture substrate

The paper shows that dimeric subunits of sHsps are functional units for substrate capture, and that dimer stability affects chaperone efficiency.

Genetic and Epigenetic Determinants Establish a Continuum of Hsf1 Occupancy and Activity Across the Yeast Genome

The paper quantifies Hsf1 binding and transcription in yeast under basal and heat shock conditions, revealing a continuum of Hsf1 occupancy and activity across the genome.

Survival protection mechanisms and genetic variability induction after stress: two sides of the same Hsp70 coin

This study shows that heat shock stress activates transposon expression by disrupting piRNA biogenesis through Hsp70's interaction with the Hsc70-Hsp90 complex in Drosophila.

HSP90 buffers newly induced mutations in massively mutated plant lines

This study shows that HSP90 reduces the penetrance of newly introduced mutations in plants, leading to fewer embryonic lethality and phenotypic abnormalities compared to HSP90-reduced plants.

Rescue of a developmental arrest caused by a C. elegans heat-shock transcription-factor mutation by loss of ribosomal S6-kinase activity

This study identifies that loss of rsks-1/S6-Kinase rescues developmental arrest in C. elegans hsf-1 mutants, revealing an unexplained regulatory link between heat-shock transcription factors and the TOR pathway during development.

The holdase function of Escherichia coli Hsp70 (DnaK) chaperone

This study investigates the holdase function of Escherichia coli DnaK chaperone using single-molecule assays, showing it stabilizes unfolded proteins and switches between holding and releasing states upon ATP binding.

Translational control plays an important role in the adaptive heat-shock response of Streptomyces coelicolor

The paper investigates transcriptional and translational changes in Streptomyces coelicolor under heat stress, revealing significant translational control and novel stress response mechanisms.

Hsf1 phosphorylation generates cell-to-cell variation in Hsp90 levels and promotes phenotypic plasticity

The paper shows that Hsf1 phosphorylation creates cell-to-cell variation in Hsp90 levels, which enhances phenotypic plasticity in budding yeast.

Allosteric Modulation of Conformational Dynamics in Human Hsp90α: A Computational Study

This study uses computational methods to analyze how ATP and ADP affect the conformational dynamics of human Hsp90, identifying key residues involved in allosteric modulation.

A Systematic Atlas of Chaperome Deregulation Topologies Across the Human Cancer Landscape

The paper systematically analyzes chaperome deregulation across 22 solid cancers using gene expression data and develops a tool to visualize proteostasis alterations.

Symmetry broken and rebroken during the ATP hydrolysis cycle of the mitochondrial Hsp90 TRAP1

The paper shows ATP hydrolysis in TRAP1 is sequential and asymmetric, with conformational flipping during the cycle to remodel client proteins.