Hideyuki Okano

Multiplexed mapping of long-range projections with FluoCode, a combinatorial fluorescent barcoding system

The paper introduces FluoCode, a fluorescent barcoding system for multiplexed projection mapping in mice and marmosets, revealing fine-scale topographic maps in subcortical regions.

DAP12-deficient microglia adopt a TREM2-independent antigen-presenting state with a glial-to-neuronal cascade in Nasu-Hakola disease

The paper profiles NHD microglia and shows they adopt an antigen-presenting state without TREM2 dependence, triggering glial-to-neuronal changes.

MECP2-mutant marmosets exhibit primate-specific phenotypes of Rett syndrome

This study generates MECP2-knockout marmosets to model Rett syndrome, revealing cortical structural and gene expression changes linked to the disease.

Generalisable signatures of anaesthesia in the large-scale functional organisation of the marmoset brain

This study shows that anaesthesia in marmosets alters large-scale brain organisation, similar to findings in humans and macaques, by constraining activity with structural connectivity and collapsing functional gradients.

Ropinirole hydrochloride mitigates oxidative stress and neuroinflammation via the PI3K-mTOR pathway in TDP-43 hiPSC-derived microglial-like cells

This study shows ropinirole hydrochloride reduces oxidative stress and neuroinflammation in TDP-43 mutant microglial-like cells by modulating the PI3K-mTOR pathway.

Design-of-Experiments for Nonlinear, Multivariate Biology: Rethinking Experimental Design through Perturb-seq

The paper evaluates experimental design in Perturb-seq, finding that correlated designs are robust in systems with signal amplification and introduces a metric to predict optimal designs.

Early pathogenesis of spinal and bulbar muscular atrophy uncovered by human iPSC-derived motor neurons highlights pathogenic neuropeptides as therapeutic targets

The paper uses human iPSC-derived motor neurons to study SBMA, identifying neuropeptides as therapeutic targets.

Selective agonism of GPR34 stimulates microglial uptake and clearance of amyloid β fibrils

This study identifies GPR34 as a regulator of microglial amyloid β fibril uptake, showing that its agonist enhances fibril clearance and that its expression declines with age and AD progression.

The Brain/MINDS 3D Digital Marmoset Brain Atlas Version 2.0: Population-Based Cortical Parcellations With Multi-Modal Standard Templates

The paper introduces BMA2.0, a population-based 3D marmoset brain atlas with multi-modal templates for improved cortical and subcortical parcellation and integration of multimodal data.

Applying functional MRI analysis techniques to whole-brain calcium-imaging data to investigate structural and functional relationships in Drosophila melanogaster

The paper applies fMRI analysis techniques to whole-brain calcium imaging data in Drosophila to study structural and functional connectivity relationships.

Dual fragmentation via collision-induced and oxygen attachment dissociations using water and its radicals for C=C position-resolved lipidomics

This study introduces OAciD, a method combining OAD and CID in negative-ion mode for C=C position-resolved lipidomics, applied to marmoset brain regions.

Chiral shift toward D-serine reflects intrathecal inflammation in multiple sclerosis and counteracts motor impairment in a murine model

The paper shows that O_SCPLOWDC_SCPLOW-serine levels are elevated in MS patients and mitigates motor deficits in a murine model.

Comprehensive profiling of anaesthetised brain dynamics across phylogeny

This study profiles neural dynamics across species during anaesthesia, finding conserved dynamical changes and linking them to molecular mechanisms.

Glial scar formation by reactive astrocytes derived from oligodendrocyte progenitor cells after closed-head injury

This study identifies that reactive astrocytes in brain injury originate from oligodendrocyte progenitor cells (OPCs), contributing to glial scar formation.

A graph-based practice of evaluating collective identities of cell clusters

The paper improves a graph-based method for evaluating cell cluster identities by addressing practical implementation issues and enhancing utility with new features and techniques.

A single-cell atlas of transcribed cis-regulatory elements in the human genome

The paper constructs a single-cell atlas of transcribed cis-regulatory elements in the human genome, revealing gene regulation patterns and trait-gene relationships across cell populations.

Proteomic characterization of aging-driven changes in the mouse brain by co-expression network analysis

This study uses quantitative proteomics to analyze age-related changes in mouse brain tissues, identifying age-associated upregulation of extracellular proteins and downregulation of synaptic proteins.

Shared GABA transmission pathology in dopamine agonist- and antagonist-induced dyskinesia

This paper identifies shared GABA transmission pathology in L-DOPA-induced and tardive dyskinesia models through structural and functional analysis of striatal neurons.

Epileptic responses in marmosets induced by knockdown of CREB Regulated Transcription Coactivator 1 (CRTC1)

This study shows that CRTC1 knockdown in marmosets leads to epileptic responses and abnormal cFOS expression, with associated neural activity changes and lesions.

Local and long-distance organization of prefrontal cortex circuits in the marmoset brain

This study maps prefrontal cortex circuits in marmosets, revealing distinct local and long-distance projection patterns and their implications for primate brain organization.

Starburst amacrine cells amplify optogenetic visual restoration through gap junctions in the murine retina

The paper demonstrates that optogenetic actuators in starburst amacrine cells enhance visual restoration in murine retinas through gap junctions.

Modeling the marmoset brain using embryonic stem cell-derived cerebral assembloids

This paper develops an in vitro marmoset brain model using cerebral assembloids to study neural development and activity.

Multi-modal brain magnetic resonance imaging database covering marmosets with a wide age range

The paper presents a large-scale MRI database of marmosets across a wide age range to study brain aging and its factors.

An efficient induction method for human spinal lower motor neurons and high-throughput image analysis at the single cell level

This paper introduces a new method to efficiently induce human spinal lower motor neurons from iPSCs and presents a single-cell evaluation system using time-lapse microscopy and machine learning.

The Japan Monkey Centre Primates Brain Imaging Repository of high-resolution postmortem magnetic resonance imaging: the second phase of the archive of digital records

The paper introduces a second phase of a brain imaging repository for nonhuman primates, providing high-resolution MRI data from nine species to support comparative neuroscience research and conservation.

Highly efficient induction of functionally mature excitatory neurons from feeder-free human ES/iPS cells

This study develops a feeder-free method to efficiently generate mature excitatory neurons from human ES/iPS cells, showing high purity and functional characteristics relevant to neurodegenerative disease research.

biPACT: a method for three-dimensional visualization of mouse spinal cord circuits of long segments with high resolution

The paper introduces a method to visualize high-resolution 3D mouse spinal cord circuits using staining and clearing techniques without genetic modification or specialized equipment.

Strategy for selective acquisition of transgenic marmosets using the piggyBac transposon system

The paper develops a method to identify transgenic marmoset embryos using a fluorescent protein indicator system based on the piggyBac transposon.

The Brain/MINDS Marmoset Connectivity Atlas: Exploring bidirectional tracing and tractography in the same stereotaxic space

The paper introduces the Brain/MINDS Marmoset Connectivity Atlas, providing a resource for exploring neuronal tracer data and tractography in a common stereotaxic space.

Developmental dynamics of the postsynaptic proteome to understand synaptic maturation and dysmaturation

The paper examines changes in PSD protein composition across development in mice and primates, linking these changes to autism spectrum disorder.