Protease and Inhibitor Mechanisms

Structure of PCPE-2 in complex with the BMP-1 metalloprotease reveals the molecular basis of its mechanism of inhibition

The paper determines the structure of PCPE-2 bound to BMP-1, revealing how PCPE-2 inhibits BMP-1 through a bipartite interaction involving its CUB domains.

Temporal N-terminomics analysis reveals proteome remodeling following brensocatib-mediated cathepsin C inhibition in promyeloblast cells

This study uses N-terminomics to show that CatC inhibition by brensocatib reduces NSP abundance and alters protease-antiprotease network dynamics in HL-60 cells.

Hck signaling drives long-distance ECM degradation through endo-exocytosis coupling in macrophages.

This study identifies HCK signaling as a driver of long-distance ECM degradation in macrophages through endo-exocytosis coupling involving MMPs and cathepsins.

Heparan sulfate selectively inhibits the collagenase activity of matrix metalloproteinase 13

This study shows heparan sulfate inhibits MMP13 collagenase activity but not non-collagen substrate degradation, revealing HS as a regulator of MMP13 through exosite binding.

AdamTS-B protease is required for morphogenesis of the Drosophila respiratory system

The paper shows AdamTS-B is essential for tracheal tube elongation and cell migration in Drosophila by remodeling basement membranes during respiratory system development.

Activity-based chemical proteomics uncovers unexpected covalent targets of E64d and reveals a role for cysteine cathepsins in PLD3 proteostasis

This study identifies cysteine cathepsins as proteases responsible for PLD3 degradation in neurons and reveals E64d's selectivity for these targets.

Polychrome activity profiling distinguishes Cys proteases and their isoforms in plants

The paper introduces polychrome activity profiling using fluorescent probes to distinguish Cys proteases and their isoforms in plants by exploiting differential probe affinity.

The pseudoproteinase iRhom2 critically promotes acute lung inflammation

This study shows that iRhom2 deficiency reduces acute lung inflammation in mice by impairing ADAM17-mediated shedding of inflammatory mediators and neutrophil recruitment.

UT-018 Protects Collagen Extracellular Matrix Through Substrate-Directed Stabilization and Collagenase Modulation

This study evaluates UT-018's ability to protect collagen from degradation by modulating collagenase activity and shielding collagen substrates, showing concentration-dependent protection in vitro.

Integrated Single-Cell and Spatial Profiling of MMP Gene Expression in Colorectal Cancer

The paper analyzes MMP gene expression in colorectal cancer using single-cell and spatial transcriptomic data, identifying cell-type-specific and spatial patterns associated with cancer progression.

A sterol-binding pocket in iRhom1 underlies paralog-specific regulation of the sheddase ADAM17

This study identifies a sterol-binding pocket in iRhom1 that regulates ADAM17 stability and shedding activity, distinct from the paralog iRhom2.

Impact of Aging, Sex, and Species on the mRNA Expression of Matrix Metalloproteinases Following Ischemic Stroke

This study examines how aging, sex, and species affect MMP mRNA expression in the brain after cerebral ischemia/reperfusion, finding significant variations across these factors.

In silico degradomics reveals disease- and endotype-specific alterations in the joint tissue landscape

The paper develops an in-silico pipeline to analyze degradomes from proteomic datasets, revealing disease- and endotype-specific tissue remodeling patterns in arthritis.

Microenvironment-specific modulation of macrophage function and tumour progression by ADAMTS1 through Syndecan-4 shedding

This study shows ADAMTS1 modulates macrophage function and tumour progression via SDC4 shedding, with model-specific effects in B16F1 and LLC tumours.

Cell-line specific role of Cathepsin B in triple-negative breast cancer growth, invasion and response to chemotherapy

This study examines the cell-line specific role of CTSB in TNBC, finding that its knockout affects viability, invasion, and chemotherapy response differently in MDA-MB-231 and MDA-MB-468 cells.

Cigarette smoking upregulates vascular expression of the novel atherosclerosis risk factor ADAMTS-7

This study shows cigarette smoke increases vascular ADAMTS7 expression through CCL17-CCR4 signaling, linking smoking to atherosclerosis via a novel gene-environment interaction.

Cryo-EM structure of CLCA1 identifies CLCA1 as a founding member of a novel metzincin family.

The paper determines the Cryo-EM structure of CLCA1, revealing its unique domain organization and regulatory mechanisms, and identifies it as a founding member of a new metzincin family.

Reverse Proteolysis Uncovers a Hidden Dimension of the Peptidome

This paper demonstrates that lysosomal cysteine cathepsins can generate fusion peptides through reverse proteolysis, revealing a new dimension of protease activity and peptide diversification.

Legumain drives processing of cathepsins and nuclear localisation of cathepsin L

This study investigates the relationship between legumain and cathepsins, showing legumain is required for cathepsin processing and nuclear localization of cathepsin L, with implications for cell proliferation and other nuclear functions.

The substrate changes matrix metalloprotease-1 dynamics, allostery, and ligand binding affinity

This paper shows collagen affects MMP1 dynamics, allostery, and ligand binding through MD simulations and virtual screening, revealing substrate-specific allosteric residues and potential drug targets.

Deciphering Features of Metalloprotease Cleavage Targets Using Protein Structure Prediction

The paper identifies structural features of ADAM10 cleavage substrates using protein structure prediction and develops a classification algorithm to predict cleavage sites.

Comprehensive protease specificity profiling

This study presents a method to comprehensively profile protease cleavage specificity using in vitro selection, high-throughput sequencing, and mass spectrometry, revealing extended spectrum preferences for broad specificity proteases.

The novel coronary artery disease risk factor ADAMTS-7 modulates atherosclerotic plaque formation by degradation of TIMP-1

This study identifies ADAMTS-7 as a risk factor for atherosclerosis by degrading TIMP-1, reducing its inhibition of MMP-9 and promoting collagen degradation.

The conserved, secreted protease inhibitor MLT-11 is necessary for C. elegans molting and embryogenesis

This study identifies MLT-11, a protease inhibitor essential for C. elegans molting and embryogenesis, showing its role in aECM remodeling and cuticle integrity.

Uncovering the evolutionary tail of GZMM: An NSP4 related protease

This study investigates the evolutionary relationship between NSP4 and GZMM, finding a closer evolutionary link between Prss57/NSP4 and Gzmm/GZMM than with other proteases.

Identification of Potential Antibody Epitopes in MMP-15

The paper identifies potential antibody epitopes in MMP-15 using bioinformatics tools, highlighting conserved and divergent regions for therapeutic targeting.

Are the Matrix Metalloproteinases 2 and 9 beeing expressed in the mucosa of the rat small intestine during its intrauterine and postnatal life development?

This study examines MMP-2 and MMP-9 expression in rat small intestine mucosa during intrauterine and postnatal development, finding no expression in epithelium but MMP-9 in mast cells postnatally.

Basolateral localization of MMP14 drives apicobasal polarity change during EMT independently of its catalytic activity

This study shows MMP14's basolateral localization drives apicobasal polarity changes during EMT independently of its catalytic activity in chick neural crest cells.