Platelet Disorders and Treatments

Super-resolution mapping of platelet α-granules uncovers non-polarized secretion and differential retention of von Willebrand Factor and thrombospondin-1

This study uses super-resolution microscopy and computational analysis to show that platelet α-granules release cargo without polarity, with distinct retention mechanisms for TSP1 and VWF.

Durable and reversible epigenetic programming of human hematopoietic stem cells as a platform for modulation of platelet function

This study uses DNA methylation-based epigenome editing to durably and reversibly silence genes in human hematopoietic stem cells, demonstrating its application in modulating platelet function.

Whole Blood Deep Immunophenotyping and Platelet-Leukocyte Aggregate Quantification by Spectral Flow Cytometry

The paper develops a 27-color spectral flow cytometry protocol to quantify platelet-leukocyte aggregates across multiple leukocyte subsets in human whole blood, revealing their distribution and behavior in health and disease.

Leptin Receptor+ cells create a perisinusoidal niche for thrombopoiesis in the bone marrow by synthesizing CXCL14

The paper identifies LepR+ cells as a niche for thrombopoiesis by producing CXCL14, which regulates lipid metabolism and terminal differentiation in megakaryocytes.

TNFα drives platelet hyperreactivity and thromboinflammation through regulation of hematopoietic stem and progenitor cells.

This study shows TNFα increases platelet reactivity and thromboinflammation by expanding and reprogramming hematopoietic stem and progenitor cells, not directly affecting megakaryocytes.

Elevated procoagulant platelets driven by necroptosis and pyroptosis aggravate pulmonary thrombosis via suppressing monocyte efferocytosis in severe pneumonia

The heart-liver axis controls platelet turnover by hepatic STAT3 phosphorylation and TPO regulation after acute myocardial infarction

Parthenolide boosts megakaryocyte maturation and restores platelet responses in Wiskott Aldrich syndrome

This study identifies parthenolide as a potential therapy for Wiskott-Aldrich syndrome by enhancing megakaryocyte maturation and platelet function in both cell and mouse models.

Patient-derived monoclonal anti-HPA-1a can induce platelet activation through FcγRIIa

The paper characterizes two anti-HPA-1a antibodies from a severe FNAIT case, showing functional differences in platelet activation through FcγRIIa engagement.

Dengue virus-induced thrombocytopenia is mediated by dysregulation of the RUNX1-MYH9 axis

This study identifies that dengue virus-induced thrombocytopenia is mediated by dysregulation of the RUNX1-MYH9 axis through reduced MYH9 expression and altered megakaryopoiesis.

Type 2B VWD substitutions trigger enhanced macrophage-mediated clearance that is attenuated by the BT200 aptamer

This study shows type 2B VWD variants bind more strongly to macrophages via LRP1 and MGL receptors, and this binding is reduced by the BT200 aptamer.

Inhibition of macrophage neuraminidase 1 protects against immune thrombocytopenia by limiting platelet clearance

This study shows that inhibiting macrophage NEU1 reduces platelet clearance in ITP by limiting phagocytosis, suggesting NEU1 as a therapeutic target.

Phosphorylation of spleen tyrosine kinase Y130 positively regulates intracellular signaling and functional responses in platelets

This study shows that phosphorylation of Syk Y130 enhances GPVI- and CLEC-2-mediated signaling and functional responses in platelets, affecting thrombosis and hemostasis.

Investigating the significance of iron levels in influencing megakaryocytic commitment in megakaryocyte-erythroid progenitors

The paper investigates how iron levels affect megakaryocytic commitment in MEPs through diet-based in vivo experiments, finding increased expression of megakaryopoiesis-related genes under iron deficiency.

A Reduced Mechanobiological Framework for Platelet Priming: From Hemodynamic Shear to Mechanosensitive Calcium Entry

The paper develops a reduced mechanobiological framework linking hemodynamic shear to mechanosensitive calcium entry in platelets, identifying a conditioning-test protocol to distinguish memory effects from other priming mechanisms.

Calibrated ADAMTS13 inhibition prevents cardiovascular shear coagulopathy

HA10, a humanized anti-ADAMTS13 antibody, preserves residual ADAMTS13 activity to prevent cardiovascular bleeding without causing thrombosis in non-human primate models.

Nitric oxide inhibits platelet adhesion to platelet-microparticles through reducing integrin αIIbβ3 activation

This study shows nitric oxide reduces PMP-induced platelet activation by inhibiting ADP release, TxA2 production, and integrin αIIbβ3 upregulation through cGMP-dependent signaling.

Droplet microfluidics reports digital and tuneable single-platelet ADP secretion describing primed, active and hyper-active states

The paper introduces DASA, a method to measure single-platelet ADP secretion, revealing distinct states of platelet reactivity and potential clinical applications.

CRISPR/Cas9-mediated deletion of Shp1 and Shp2 reveals distinct roles in human megakaryopoiesis and proplatelet formation

This study uses CRISPR/Cas9 to delete Shp1 and Shp2 in human CD34+ cells, showing Shp2 is critical for megakaryopoiesis and thrombopoiesis while Shp1 is not.

Sub-endothelial platelet activation amplifies neutrophil transmigration within venular walls

The paper identifies a platelet-neutrophil circuit that enhances neutrophil transmigration through venular walls by releasing CXCL7.

Burst-like Secretion of Platelet Dense Granules Promotes Thrombus Shell Expansion

The paper shows that platelet dense granules are released in bursts driven by calcium spikes and extracellular feedback, enhancing thrombus expansion.

Hidden Complexity of Pediatric Platelet Disorders: Functional Diversity and Unexpected Hypercoagulable Phenotypes

This study identifies functional diversity and unexpected hypercoagulable phenotypes in pediatric platelet disorders, revealing complexity beyond traditional diagnostic categories.

A genome-wide meta-analysis identifies a sex-specific genetic effect for platelet aggregation in response to agonists

This study identifies a sex-specific genetic variant affecting platelet aggregation in response to low-dose epinephrine across multiple cohorts.

Diabetes impacts endothelial Weibel-Palade body biogenesis and VWF secretion

This study shows that chronic diabetes, not hyperglycaemia alone, impairs Weibel-Palade body biogenesis and VWF secretion in endothelial cells, contributing to vascular dysfunction.

Regulator of G Protein Signaling 6 Negatively Regulates Platelet Activation and Arterial Thrombosis in Mice

This study shows that RGS6 inhibits platelet activation and arterial thrombosis by modulating P2Y12/Gi signaling in mice.

SARS-CoV-2 spike protein-associated sialoglycoconjugates induce nanoscale filipodia to facilitate micro-size platelet clotting

This study shows that SARS-CoV-2 spike protein-associated sialoglycoconjugates induce nanoscale filipodia in platelets, leading to clotting and thrombosis.

An imaging flow cytometry method to study platelet-monocyte aggregates using Long COVID as a model

This study uses imaging flow cytometry to quantify platelet-monocyte aggregates in Long COVID patients and healthy controls, finding significantly elevated levels and altered attachment patterns in patients.

A Novel VWF Knockout Endothelial Cell Model to Study Von Willebrand Factor Biology and Von Willebrand Disease Mechanisms

The paper introduces a VWF-knockout endothelial cell model to study VWF variants and their impact on von Willebrand disease, showing it can distinguish pathogenic from non-pathogenic variants.

Denatured Albumin Gains a Function of Regulating Platelet Activity

This study shows denatured serum albumin can regulate platelet activity by binding to integrin IIbβ3, similar to fibrinogen, under conditions like alcohol intake or oxidative stress.

Plasmin-mediated cleavage of GPIbα contributes to breakdown of platelet-von Willebrand factor complexes

This study shows plasmin cleaves GPIbα, disrupting platelet-VWF complexes, but not VWF itself, and suggests GPIb shedding may be plasmin-mediated in TTP.