Advanced Electron Microscopy Techniques and Applications

Effects of base temperature, immersion medium, and EM grid material on devitrification thresholds in cryogenic optical super-resolution microscopy

This study investigates how cooling temperature, immersion medium, and EM grid material affect devitrification in cryo-SMLM, showing that metallic supports in cold nitrogen tolerate higher laser intensities.

Automated Removal of Corrupted Tilts in Cryo-Electron Tomography

This study develops a deep learning method to automatically remove corrupted tilts in cryo-ET, showing high efficiency and reliability with trained models for direct application.

A Robust Hierarchical Linear Model for Cryo-EM Map Analysis

The paper introduces a robust hierarchical linear model for analyzing cryo-EM maps and atomic structures, showing it can capture atom-specific signal features more accurately than existing methods.

Deep mapping of the endomembrane system of cerebellar Purkinje neurons

The paper maps the endomembrane system of cerebellar Purkinje neurons using electron microscopy, revealing cell-type-specific and subcellular structural organization of organelles.

Q-score as a reliability measure for protein, nucleic acid, and small molecule atomic coordinate models derived from 3DEM density maps

The paper introduces and analyzes Q-scores to assess the reliability of atomic coordinate models fitted to 3DEM maps, showing their correlation with map resolution and model quality.

Foam film vitrification for cryo-EM

This paper introduces a foam film vitrification method for cryo-EM that improves ice thickness control and particle orientation, simplifying specimen preparation.

Structure of the complete extracellular bacterial flagellum reveals mechanism for flagellin incorporation

This study determines the structure of the complete extracellular bacterial flagellum, revealing mechanisms of flagellin incorporation and filament assembly.

Molecular model of a bacterial flagellar motor in situ reveals a "parts-list" of protein adaptations to increase torque

The paper determines the in situ structure of a high-torque bacterial flagellar motor using cryogenic electron microscopy, revealing protein adaptations and universal principles of torque generation.

Development of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography

This paper develops an ultrafast pulsed ponderomotive phase plate to enhance contrast in cryo-ET by synchronizing electron packets with laser pulses.

Cryogenic Electron Tomography Redefines Herpesvirus Capsid Assembly Intermediates Inside the Cell Nucleus.

The paper uses cryogenic electron tomography to redefine herpesvirus capsid assembly intermediates inside the cell nucleus by deriving atomic models and revealing structural heterogeneity in capsid components.

CryoDRGN-AI: Neural ab initio reconstruction of challenging cryo-EM and cryo-ET datasets

The paper introduces cryoDRGN-AI, a method for ab initio 3D reconstruction of cryo-EM and cryo-ET datasets, revealing new conformational states and unresolved motions.

Unsupervised Multi-scale Segmentation of Cellular Cryo-electron Tomograms with Stable Diffusion Foundation Model

The paper introduces an unsupervised method for segmenting subcellular structures in cryo-ET images using a Stable Diffusion model, achieving performance close to expert annotations.

A two-component quasi-icosahedral protein nanocompartment with variable shell composition and irregular tiling

The paper characterizes a two-component encapsulin from Streptomyces lydicus, showing differential assembly and mixed shell formation with irregular tiling.

Electron diffraction captures high-resolution structures from in vivo protein nanocrystals of Bacillus thuringiensis

This study uses electron diffraction techniques to determine the structure of in vivo protein nanocrystals from Bacillus thuringiensis, showing they are viable alternatives to X-ray free electron lasers.

Straightening FSC curves

The paper introduces a new scale for plotting FSC curves that ensures consistent statistics and improves reliability of high-resolution values.

In-Situ High-Resolution Cryo-EM Reconstructions from CEMOVIS

The paper compares CEMOVIS and FIB-milling for cryo-EM sample preparation, showing CEMOVIS provides more imageable area with comparable structural preservation.

FLUID-CELL: Flow-enabled Light and Ultrastructural Imaging Device for Correlative Electron and Light Localization

The paper introduces a microfluidic device that enables correlative fluorescence light microscopy and cryo-electron microscopy for real-time and ultrastructural analysis of cells.

Charting the molecular landscape of neuronal organisation within the hippocampus using cryo electron tomography

Xenon plasma FIB cryo lift-out from high-pressure-frozen mouse hippocampal slices up to 200 µm thick enables fluorescence-guided cryoET of CA1 synapses and apical dendrite cytoskeleton.

Pulsed laser lensing for phase modulation in electron microscopy

This paper demonstrates pulsed laser lensing to modulate electron beam phase in microscopy, achieving a 430 rad phase shift and angular deflection of 45 microrad.

Constrained template matching using rejection sampling

This paper introduces rejection sampling to improve macromolecular complex detection in cryo-electron tomography by incorporating biological constraints, achieving performance comparable to deep learning methods without annotated data.

SMART: An Integrated Platform Revolutionizing Cryo-Electron Microscopy Workflow from Data Acquisition to Atomic Modeling

SMART is an integrated cryo-EM platform that automates data acquisition, reconstruction, and atomic modeling using AI, enabling users to generate high-resolution structures like TRPML1 at 2.1 Å resolution.

Elucidating the dynamics of Integrin αIIb3 from native platelet membranes by cryo-EM with build and retrieve method

This paper uses cryo-EM with the Build-and-Retrieve method to solve integrin αIIbβ3 structures from resting human platelet membranes, revealing new insights into its regulation and dimer conformation.

Amortized template-matching of molecular conformations from cryo-electron microscopy images using simulation-based inference

This paper introduces cryoSBI, a method using simulation-based inference to infer biomolecular conformations from cryo-EM images, achieving fast and accurate results.

Reduction of SEM charging artefacts in native cryogenic biological samples.

This paper reduces SEM charging artefacts in cryogenic biological samples using interleaved scanning, enabling clearer visualization of previously masked structures.

Imaging of specialized plant cell walls by improved cryo-CLEM and cryo-electron tomography

The paper improves cryo-CLEM and cryo-ET techniques for imaging plant cell walls with enhanced precision and reduced contamination, enabling detailed structural analysis of root-specific features.

High-throughput MicroED for probing ion channel dynamics

This study uses high-throughput MicroED to analyze structural dynamics of NaK and NaK2CNG ion channels, revealing sodium binding and dynamic selectivity filter behavior.

Development of a Charge-Resistant Embedding Media for High-Performance Serial Block Face Imaging of Cells and Tissues

This paper develops a PEG-doped resin to reduce specimen charging in SBEM, improving imaging quality without sacrificing resolution or physical properties.

Cryo-EM structure of the vault from human brain reveals symmetry mismatch at its caps

The paper presents the cryo-EM structure of vault particles from human brain tissue, revealing a symmetry mismatch at their caps and its implications for therapeutic modifications.

Building molecular model series from heterogeneous CryoEM structures using Gaussian mixture models and deep neural networks

This paper introduces a method to generate molecular model series from CryoEM datasets, capturing conformational dynamics with high geometric accuracy.

De Novo Hydration of Cryo-EM Reconstructions through Molecular Dynamics Simulations of the Excess Chemical Potential

This paper introduces a method using molecular dynamics simulations to predict water positions in cryo-EM maps by analyzing excess chemical potential, showing high agreement with experimental data.