Telomeres, Telomerase, and Senescence

A 4-base pair core-enclosing helix in telomerase RNA is essential and binds to the TERT catalytic protein subunit

The paper identifies a 4-base pair core-enclosing helix in telomerase RNA essential for binding TERT and telomerase activity in yeast.

Novel Dual Labeled Fluorescence Probe Based Assay to Measure the Telomere Length

The paper develops a dual-labeled fluorescence probe qPCR assay to measure telomere length, improving accuracy over SyBr Green by specific targeting of telomeric DNA.

Determinants of telomere length across human tissues

The paper examines telomere length variation across 6,391 tissue samples from 952 individuals, showing tissue-specific differences, positive correlations between tissues, and associations with age and ancestry.

Telomere length set point regulation in human pluripotent stem cells critically depends on the shelterin protein TPP1

This study shows that TPP1 isoforms and specific residues regulate telomere length set point in human pluripotent stem cells, with TPP1L compensating for TPP1S loss and affecting telomere stability and elongation.

PROFILING OF Y1 CELLS TREATED WITH FGF-2 REVEALS PARALLELS WITH ONCOGENE-INDUCED SENESCENCE

The paper profiles Y1 cells treated with FGF-2, revealing epigenetic and transcriptomic changes consistent with oncogene-induced senescence.

The PNUTS-PP1 axis regulates endothelial aging and barrier function via SEMA3B suppression

This study identifies the PNUTS-PP1 axis as critical for endothelial aging and barrier function, showing that SEMA3B suppression mediates these effects.

Tousled-like kinase activity is required for transcriptional silencing and suppression of innate immune signaling

This study shows TLK1/2 activity protects replication forks, contributes to chromatin silencing, and suppresses innate immune responses, with implications for immune evasion in chromosomally unstable cancers.

Persistent telomere cohesion protects aged cells from premature senescence

The paper shows that TRF1 deficiency protects aged cells from premature senescence by maintaining telomere cohesion and preventing DNA damage.

The Role of Rif1 in telomere length regulation is separable from its role in origin firing

The paper investigates whether telomere length regulation by Rif1 is linked to telomeric origin firing, finding they are separable functions.

Telomere length and oxidative stress variations in a murine model of Alzheimer's disease progression

The paper examines telomere length and oxidative stress in a 3xTg-AD murine model, finding shorter telomeres in blood cells of older transgenic mice and increased oxidative stress with age, but no correlation between oxidative stress and telomere length.

A Synthetic Single-Stranded Dual-Template Oligonucleotide as a Reference Standard for Monochrome Multiplex qPCR Measurements of Average Telomere Length

Single-molecule imaging of telomerase RNA reveals a Recruitment-Retention model for telomere elongation

This study uses single-molecule imaging to reveal a Recruitment-Retention model for telomere elongation, showing how hTERT and TPP1 regulate hTR dynamics and how mutations affect telomere homeostasis.

The human SKI complex prevents DNA-RNA hybrid-associated telomere instability

This study shows the human SKI complex prevents telomere instability by resolving DNA-RNA hybrids at telomeres during G2 phase.

Differential Levels of Telomeric Oxidized Bases and TERRA Transcripts in Childhood Autism

The paper investigates telomere length and oxidative damage in children with autism, finding higher oxidative base lesions and variable TERRA expression between sexes.

Evolutionary and biochemical analyses reveal conservation of the Brassicaceae telomerase ribonucleoprotein complex

The paper investigates the evolution and function of telomerase in Brassicaceae plants, finding that TER1 and TER2 are not functional telomerase RNAs and identifying a conserved telomerase RNA locus.

SMCHD1 Promotes ATM-dependent DNA Damage Signaling and Repair of Uncapped Telomeres

This study shows SMCHD1 is required for ATM-dependent DNA damage signaling and repair of uncapped telomeres, with its depletion reducing telomere fusions in TRF2-depleted cells.

Genomic footprints of activated telomere maintenance mechanisms in cancer

The paper analyzes whole-genome sequencing data from cancer samples to identify genomic footprints of telomere maintenance mechanisms, finding specific telomere variant repeat patterns associated with ATRX/DAXX mutations.

Telomere relocalization to the nuclear pore complex in response to replication stress

The paper investigates how mutant POT1 alleles cause replication stress and telomere fragility, revealing a role for the nuclear pore complex in resolving these defects and telomere repositioning to the nuclear periphery.

Universal assay for measuring vertebrate telomeres by real-time quantitative PCR

The paper develops universal reference primers for qPCR-based telomere length measurement in any vertebrate species.

RADX Sustains POT1 Function at Telomeres to Counteract RAD51 Binding, which Triggers Telomere Fragility

The paper identifies RADX as a telomere-binding protein that suppresses telomere fragility by counteracting RAD51 binding, alongside POT1.

Dasatinib Plus Quercetin on Uterine Age-Related Dysfunction and Fibrosis in Mice

This study investigates the effects of dasatinib plus quercetin on uterine fibrosis in aged mice, showing increased p53 expression and reduced miR34a levels.

The DNA end-binding protein Ku associates with human telomeres primarily via protein-protein interactions

This study shows that Ku proteins associate with human telomeres mainly through protein-protein interactions, not DNA binding.

T-loops Refold after Telomerase Extension and Unfold Again at Late S/G2 for C-strand Fill-in

The paper investigates t-loop dynamics during S phase, showing they unfold twice and persist throughout the phase.

Oxidative stress, telomere length, and frailty in an old age population

The paper examines the relationship between oxidative stress, telomere length, and frailty in older adults, finding an association between telomere length and frailty but not between oxidative stress and these factors.

Tel1 activation by the MRX complex is sufficient for telomere length regulation but not for the DNA damage response in S. cerevisiae

The paper shows that Tel1 activation by the MRX complex regulates telomere length but not the DNA damage response in S. cerevisiae, challenging existing models.

Loss of SATB1 Induces a p21 Dependent CellularSenescence Phenotype in Dopaminergic Neurons

This study shows that loss of SATB1 in dopaminergic neurons leads to cellular senescence through p21 activation, suggesting a role for senescence in Parkinson's disease pathology.

A Multidimensional Systems Biology Analysis of Cellular Senescence in Ageing and Disease

The paper curates a database of senescence-associated genes and performs systems biology analyses to identify novel regulators and pathways of cellular senescence.

RNA-DNA hybrids support recombination-based telomere maintenance in fission yeast

This study investigates telomere maintenance in fission yeast lacking telomerase, showing that TERRA RNA and shelterin component changes support recombination-based telomere maintenance.

TIN2 functions with TPP1/POT1 to stimulate telomerase processivity

This study identifies a new TIN2 isoform, TIN2M, and shows that all TIN2 isoforms enhance telomerase processivity beyond TPP1/POT1, suggesting TIN2 is a functional component of the TPP1/POT1 complex.

Loss of Ku’s DNA end binding activity affects telomere length via destabilizing telomere-bound Est1 rather than altering TLC1 homeostasis

This study shows that reduced Ku's DNA end binding activity shortens telomeres by destabilizing Est1-telomere interactions, not by affecting TLC1 levels.