Telomeres, Telomerase, and Senescence

Long-read sequencing reveals telomere inheritance patterns from human trios

The paper uses long-read sequencing to analyze telomere inheritance patterns in human trios, finding parent-of-origin effects and heritable influences on telomere length.

Standard treatment against paediatric BRAF-V600E glioma promotes senescence and sensitizes tumours to BCL-xL inhibition

This study shows that standard therapies for paediatric BRAF-V600E glioma induce senescence, which can be targeted by BCL-xL inhibitors to prevent tumour regrowth.

Spontaneous replication fork collapse regulates telomere length homeostasis in wild type cells

The paper shows that telomerase elongates a second substrate formed by spontaneous replication fork collapse in budding yeast, significantly contributing to telomere length homeostasis.

Senotherapeutic potential against xeroderma pigmentosum

This study identifies senomorphic and senolytic agents that counteract UV-induced senescence in XP-derived melanocytes, reducing SASP gene expression and related pathway activation.

Identification of senescence-associated drivers of tumour growth and progression using a novel microarray platform

This study identifies SASP factors that drive tumorigenesis in breast and lung cancer cells using a microarray platform and validates their role in growth and survival.

UHRF1 Overexpression Generates Distinct Senescent States with Different Tp53 Dependencies

This study shows UHRF1 overexpression in zebrafish hepatocytes leads to diverse senescent states with different Tp53 dependencies and responses to senolytic drugs.

Senescence-directed nanotherapy ameliorates fibrosis and overcomes immune exclusion in cancer

P-selectin-targeted fucoidan nanoparticles carrying Navitoclax or dBET6 deplete a senescent-like, lipid-associated macrophage subset, reverse mouse liver and lung fibrosis with less toxicity, and restore checkpoint therapy response in fibrotic tumors.

Telomere length of both parents contributes to heritable POT1 cancer-predisposition syndrome

The paper investigates how POT1 mutations affect telomere inheritance and cancer risk by analyzing telomere length in families with POT1 mutations.

Salvianolic acids are natural senolytics and increase lifespan in old age

This study identifies salvianolic acids as natural senolytics that enhance lifespan and healthspan in aged mice by targeting senescent cells through redox modulation and inducing apoptosis and ferroptosis.

Fission yeast RPA-TERT-Tpz1TPP1 complex promotes telomere extension and suppresses telomere recombination

This study identifies a RPA-Trt1TERT-Tpz1TPP1 complex in fission yeast that promotes telomere extension and suppresses recombination through specific protein interactions.

Telomere variant sequences encode the genetic blueprint for allele-specific telomere length

The paper identifies heritable telomere variant sequences that determine allele-specific telomere length and shows their role in telomere maintenance and inheritance.

Machine learning-based definition of cellular senescence reveals pro-senescence potential implication in lung adenocarcinoma

This study introduces PreCSenM, a machine learning model that quantifies cellular senescence and identifies HDAC inhibitors as senescence inducers in lung adenocarcinoma.

FAβ-gal: an automated fluorescence-based quantification of the senescence-associated beta-galactosidase X-gal assay

This paper introduces FAβ-gal, a fluorescence-based method to quantify SA-β-gal activity using X-gal, improving accuracy and sensitivity over traditional colorimetric assays.

Fragmentation Patterns of Human Telomeric Chromatin in Plasma cfDNA

This study shows that cfDNA can map telomere chromatin structure, revealing age-related and disease-specific changes in telomeric footprints and gene signatures.

Telomeric amplicons of SUL1 and Y' in yeast are generated by microhomology-mediated break induced replication occurring in cis

The paper identifies a mechanism of gene amplification in yeast involving microhomology-mediated break-induced replication (mmBIR) at telomeres, leading to tandem duplication of chromosomal ends.

Targeting CyclinD1-CDK6 to Mitigate Senescence-Driven Inflammation and Age-Associated Functional Decline

This study shows that targeting CCND1-CDK6 reduces DNA damage, inflammation, and age-related decline in mice by suppressing SASP and ISG expression.

Suppression of early pro-inflammatory senescent signature post-radiotherapy mitigates chronic bone damage

This study shows that early suppression of the pro-inflammatory senescent secretome (SASP) with ruxolitinib reduces chronic bone damage after radiotherapy.

La1: an evolutionarily conserved player in the Arabidopsis telomerase complex

This paper identifies AtLa1 as an essential RNA-binding factor for Arabidopsis telomerase function, revealing its role in telomerase complex assembly and highlighting evolutionary conservation with yeast and ciliate proteins.

Characterizing the SASP-Dependent Paracrine Spreading of Senescence Between Human Brain Cell Types

The paper identifies cell-type-specific SASP ligand-receptor pairs that mediate paracrine senescence spreading in human brain cells and shows that targeting these factors can prevent senescence spread.

Disease mutations in the N-terminal extension and α 2'-helix of dyskerin do not abolish dimerization but disrupt binding to the telomerase RNA

The paper shows that disease mutations in dyskerin's N-terminal extension and α2-helix do not prevent dimerization but impair hTR binding, contributing to X-DC pathogenesis.

Human length telomeres restrict the regenerative potential of hematopoietic stem cells in mice

The paper shows that human-length telomeres in mice restrict hematopoietic stem cell regenerative potential under stress conditions.

A CRISPR/Cas9-induced blunt-end telomere system in S. pombe reveals RNase H2-dependent RNA primer removal at the terminal Okazaki fragment of lagging telomeres

The paper introduces a CRISPR/Cas9 system in S. pombe to study telomere replication, revealing RNase H2-dependent RNA primer removal in lagging-strand telomeres.

Senescent cells cluster CTCF on nuclear speckles to sustain their splicing program

This paper identifies senescence-induced CTCF clusters (SICCs) and shows they sustain the senescence splicing program by repurposing nuclear speckle components.

Lysosomal Profiling with LysoTracker for Quantitative Assessment of Cellular Senescence in Human Fibroblasts

This paper introduces a live-cell imaging protocol using LysoTracker Deep Red to quantify lysosomal remodeling as a marker of cellular senescence in human fibroblasts.

Molecular and functional characterization of telomeric repeat-containing RNAs in Chinese hamster ovary cells

This study characterizes TERRA and ARIA telomeric RNAs in CHO cells, showing ARIA regulates telomeric DNA integrity by preventing ssDNA formation at ITSs.

Oncogene inactivation-induced senescence facilitates tumor relapse

This study shows that oncogene inactivation-induced senescence both limits tumor growth and promotes relapse by creating a pro-recurrence microenvironment.

Esc1-mediated anchoring regulates telomere clustering in response to metabolic changes.

The paper identifies Esc1-mediated telomere anchoring as a mechanism regulating telomere clustering in yeast during metabolic shifts to quiescence.

Inhibition of lagging strand replication by G-rich telomeric DNA and the shelterin subunit POT1

This study investigates how telomeres impede replication by showing G-rich DNA and shelterin subunit POT1 block lagging strand replication, with POT1 causing Okazaki fragment skipping and large single-stranded gaps.

Inherited long telomeres induce a genome-wide transcriptional response in budding yeast

This study shows that inherited long telomeres in budding yeast cause a genome-wide transcriptional response, affecting gene expression across the genome.

Matrix stiffness induces endothelial network senescence

This study shows matrix stiffness induces endothelial cell senescence through a Notch-JNK-FOS pathway, supported by in vitro models and patient tissue analysis.