Chemokine receptors and signaling

Evolutionary conservation and divergence of CXCL17 orthologs: functional evidence in reptiles and loss in the avian lineage

This study identifies functional CXCL17 orthologs in reptiles and shows their loss in birds, using bioinformatics and recombinant protein assays.

Using endocytosis to switch between chemoattraction and chemorepulsion

The paper models how B cells switch between being attracted and repelled by CCL19 through receptor internalization, showing that changing concentration or inhibiting internalization alters cell direction.

Eosinophils promote monocyte to macrophage differentiation and anti-bacterial immunity

This study shows that ACKR2 regulates eosinophil levels in bone marrow, affecting monocyte differentiation and anti-bacterial immunity.

MDA-MB-231 cell morphology influences chemotactic sensing of CXCL12 gradients in type 1 bovine collagen matrix

This study shows that MDA-MB-231 cell morphology affects their chemotactic response to CXCL12 gradients in 3D collagen matrices.

CXCL13-CXCR5 Signaling in CD8⁺ T Cell Recruitment and Lymphoid Immune Organization in Clear Cell Renal Cell Carcinoma

This study identifies CXCL13-CXCR5 signaling as critical for CD8 T cell recruitment and immune organization in ccRCC, with potential as a biomarker and therapeutic target.

Physical interaction with Ephrin B1 promotes CXCR4 intracellular localization and oncogenic potential

This study identifies Ephrin B1 as a key mediator of CXCR4 tumorigenic signaling by promoting its intracellular localization and inhibiting surface expression and signaling.

Ancestral Origin of the CXCL17-GPR25 System Traced to the Lobe-Finned Fish Latimeria chalumnae

This study identifies a functional CXCL17-GPR25 system in the coelacanth, supporting its evolutionary origin in ancient fishes and transmission to vertebrates.

Identification and Functional Characterization of CXCL17 Orthologs in Amphibians

This study identifies and functionally characterizes CXCL17 orthologs in amphibians, revealing conserved mechanisms of GPR25 receptor activation.

CXCR1 and CXCR2 display receptor bias for shared chemokine agonists

The paper shows that CXCL1 acts as a G protein-biased partial agonist at CXCR1 but a balanced full agonist at CXCR2, revealing receptor bias in chemokine signaling.

Identification and functional characterization of two CXCL17 paralogs from zebrafish

This study identifies and functionally characterizes two CXCL17 paralogs in zebrafish, showing they bind and activate GPR25 and induce chemotaxis.

Constitutive activity of an atypical chemokine receptor revealed by inverse agonistic nanobodies

This study uses inverse agonistic nanobodies to reveal constitutive activity of ACKR3, showing it drives non-canonical signaling and basal cell motility, suggesting therapeutic potential.

CXCL17 activates three MAS-related G protein-coupled receptors independently of its conserved C-terminal fragment

This study confirms that CXCL17 activates MRGPRX2, MRGPRX1, and MAS1 independently of its C-terminal fragment, unlike its effect on GPR25.

Inhibition of constitutive activity of the atypical chemokine receptor ACKR3 by the small-molecule inverse agonist VUF16840

This study characterizes VUF16840 as a selective inverse agonist of ACKR3 that inhibits constitutive and chemokine-induced signaling by stabilizing the inactive conformation of the receptor.

Neutrophil-chemoattractant CXCL5 induces lung barrier permeability in acute lung injury

This study identifies CXCL5 as a factor that increases alveolar-epithelial barrier permeability in acute lung injury, independent of neutrophil recruitment.

Rules of engagement: determinants of chemokine receptor activation and selectivity by CCL27 and CCL28

This study identifies N-terminal determinants of CCL27 and CCL28 receptor activation and selectivity by analyzing mutations and using AlphaFold models.

Human CXCL17 Activates and Binds to Fish GPR25 Orthologs

This study shows human CXCL17 activates and binds to fish GPR25 orthologs, suggesting conservation of the CXCL17-GPR25 interaction across vertebrates.

Noncanonical roles of chemokine regions in CCR9 activation revealed by structural modeling and mutational mapping

This study identifies a non-canonical activation mechanism of CCR9 by CCL25 through structural modeling and mutational analysis, revealing the 30s loop as the key activation region.

CXCL12 chemokine dimer signaling modulates acute myelogenous leukemia cell migration through altered receptor internalization

This study shows that CXCL12-LD, a dimeric variant of the chemokine, reduces chemotaxis in AML cells by altering receptor signaling and internalization, suggesting potential for improved CXCR4 targeting.

Intestinal Epithelial C/EBPβ Deficiency Impairs Colitis-Associated Tumorigenesis by Disrupting CXCL1 /CXCL2/CXCL5-CXCR2-Mediated Neutrophil Infiltration

This study identifies C/EBPβ as a key driver of colitis-associated tumorigenesis by promoting neutrophil infiltration via the CXCL1/CXCL2/CXCL5-CXCR2 axis, suggesting it as a therapeutic target.

Disruption of basal CXCR4 oligomers impairs oncogenic properties in lymphoid neoplasms

This study shows that disrupting CXCR4 oligomers in lymphoid cancers reduces migration, survival signaling, and tumor growth, while sensitizing cells to venetoclax.

Inflammatory chemokine receptors CCR1, CCR2, CCR3 and CCR5 are essential for an optimal T cell response to influenza.

This study shows that CCR1/2/3/5 deletion reduces CD4 T cell responses but not CD8 T cell responses to influenza virus infection.

The Dual Roles of the CXCL10-CXCR3 Axis and Its Therapeutic Potential in Osteosarcoma

This study investigates the dual role of the CXCL10-CXCR3 axis in osteosarcoma, showing it promotes tumor growth but also recruits immune cells, suggesting CXCR3 inhibition could target metastasis.

GPR15LG binds CXCR4 and synergistically modulates CXCL12-induced cell signaling and migration

This study shows GPR15LG binds CXCR4, enhancing CXCL12-induced signaling and promoting cell migration in immune and cancer cells.

Identification of the orphan GPR25 as a receptor of the chemokine CXCL17

This study identifies GPR25 as the receptor for CXCL17 using AlphaFold 3 prediction and NanoBiT-based assays, showing specific activation and structural binding.

A fluorescent probe enables the discovery of improved antagonists targeting the intracellular allosteric site of the chemokine receptor CCR7

This study develops a fluorescent ligand to identify improved antagonists targeting the intracellular allosteric site of CCR7, showing their effectiveness in both recombinant and endogenous settings.

Ex vivo model of breast cancer cell invasion in live lymph node tissue

This paper develops an ex vivo model of breast cancer cell invasion in live lymph node tissue, revealing preferential invasion patterns and immune-related resistance mechanisms.

A Potent Nonapeptide Inhibitor for the CXCL12/HMGB1 heterocomplex: A Computational and Experimental Approach

The paper computationally optimizes a peptide inhibitor of the CXCL12/HMGB1 heterocomplex, resulting in a variant with significantly improved affinity and activity.

Traveling wave chemotaxis of neutrophil-like HL-60 cells

This study investigates how neutrophil-like HL-60 cells respond to traveling waves of chemoattractants, finding that cell movement depends on wave speed and involves different signaling pathways at the front and back of the wave.

Immune disease dialogue of chemokine-based cell communications as revealed by single-cell RNA sequencing meta-analysis

The paper performs a meta-analysis of scRNA-seq data to identify disease-specific and shared chemokine-based cell communication patterns in immune diseases.

Evolutionary diversity of CXCL16-CXCR6: Convergent Substitutions and Recurrent Gene Loss in Sauropsids

This study examines the evolutionary diversity of the CXCL16-CXCR6 axis across vertebrates, identifying motif substitutions and recurrent gene loss in various species.