W. Chen

Lineage-specific regulatory changes in the pathological cardiac remodeling of hypertrophy cardiomyopathy unraveled by single-nucleus RNA-seq and spatial transcriptomics

This study uses single-nucleus RNA-seq and spatial transcriptomics to identify lineage-specific regulatory changes and candidate therapeutic targets in hypertrophy cardiomyopathy.

Botrytis cinerea BcPTP1 is a late infection phase, cysteine rich protein cytotoxic effector

This study identifies BcPTP1 as a phytotoxic effector secreted by Botrytis cinerea during late infection, which induces cell death and promotes lesion spreading.

Intracellular Lipid Droplet Accumulation Occurs Early Following Viral Infection and Is Required for an Efficient Interferon Response

The paper shows that lipid droplets form early after viral infection, support interferon production, and reduce viral replication.

Structural insights into the recognition of histone H3Q5 serotonylation by WDR5

This study identifies WDR5 as a reader of H3Q5ser, showing it binds to this modification and promotes transcription in neuroblastoma cells, suggesting a role in tumorigenesis.

Stem cell-free therapy for glaucoma to preserve vision

This study demonstrates that stem cell-derived secretome reduces intraocular pressure and protects retinal ganglion cells in glaucoma models through COX2-PGE2 pathway activation and extracellular matrix remodeling.

BSCL2/Seipin Deficiency in Heart Causes Energy Deficit and Heart Failure via Inducing Excessive Lipid Catabolism

This study shows that BSCL2 deficiency in the heart causes heart failure through excessive lipid catabolism, reduced energy reserves, and ATP levels, which can be mitigated by inhibiting fatty acid oxidation or high-fat diet.

Molecular classification reveals the diverse genetic features and prognosis of gastric cancer: a multi-omics consensus ensemble clustering

This study identifies two molecular subtypes of gastric cancer using multi-omics data and clustering algorithms, showing distinct survival outcomes and therapeutic responses.

Postnatal lymph node expansion of stromal progenitors towards reticular and CD34+ stromal cell subsets is determined by distinct transcriptional programs

The paper identifies distinct transcriptional programs driving the expansion of stromal progenitors in mesenteric lymph nodes, revealing Irf3's role in FRC differentiation and microbiota-independent epigenomic signatures.

Development and Application of Cas13a-based Diagnostic Assay for Neisseria Gonorrhoeae Detection and Identification of Azithromycin Resistance

This paper develops a Cas13a-based assay for detecting Neisseria gonorrhoeae and identifying azithromycin resistance mutations, showing high sensitivity and concordance with existing methods.

Permeability profiling of all 13 Arabidopsis PIP aquaporins using a high throughput yeast approach

The paper develops a high-throughput yeast assay to profile permeability of all 13 Arabidopsis PIP aquaporins to various substrates and links results to their biological roles and structural features.

Kaposi's Sarcoma-associated Herpesvirus vFLIP Promotes MEndT to Generate Hybrid M/E State for Tumorigenesis

This study shows KSHV infection of MSCs drives MEndT to generate hybrid M/E state cells, which are tumorigenic and recapitulate KS features, with vFLIP as a key regulator.

Myofibroblast transcriptome indicates SFRP2+ fibroblast progenitors in systemic sclerosis skin

The paper identifies SFRP2+ fibroblast progenitors as the source of myofibroblasts in systemic sclerosis skin using single-cell RNA sequencing.

Kaposi's Sarcoma-associated Herpesvirus Promotes Mesenchymal-to-Endothelial Transition by Resolving the Bivalent Chromatin of PROX1 Gene

This study shows KSHV resolves the bivalent chromatin of PROX1 to activate its expression, promoting MEndT and tumorigenic features in MSCs.

Establishment and characterization of fibroblast cultures derived from a female common hippopotamus (Hippopotamus amphibius) skin biopsy

This study establishes and characterizes fibroblast cultures from a female common hippopotamus, confirming their origin and viability for genetic preservation.

New metabolic alterations and predictive marker pipecolic acid in sera for esophageal squamous cell carcinoma

This study identifies 12 serum metabolites associated with esophageal squamous cell carcinoma and highlights pipecolic acid as a predictive biomarker for tumorigenesis.

In vivo co-registered hybrid-contrast imaging by successive photoacoustic tomography and magnetic resonance imaging

This paper presents a hardware-software solution for co-registering photoacoustic tomography and MRI images in vivo, demonstrating hybrid-contrast imaging of tumor microenvironments.

Single-molecule conformational dynamics of a transcription factor reveals a continuum of binding modes controlling association and dissociation

This study uses single-molecule FRET to show that NF-κB exhibits a continuum of binding modes that control its association and dissociation from DNA, with IκB modulating these dynamics.

Evolutionary Analysis of Transcriptional Regulation Mediated by Cdx2 in Rodents

This study analyzes evolutionary changes in Cdx2's DNA binding and transcriptional regulation between mice and rats, finding conserved transcriptional output despite divergent DNA binding.

Selective inhibition reveals the regulatory function of DYRK2 in protein synthesis and calcium entry

This study develops a selective DYRK2 inhibitor, C17, and identifies novel targets like 4E-BP1 and STIM1, revealing DYRK2's role in protein synthesis and calcium entry.

The Prognostic Value of Tumor-Infiltrating Immune Cells in Gynecologic Cancers

This study characterizes immune infiltration patterns in ovarian, cervical, and uterine cancers and identifies immune subtypes associated with prognosis.

Neutralization of SARS-CoV-2 lineage B.1.1.7 pseudovirus by BNT162b2 vaccine-elicited human sera

The paper assesses the neutralizing activity of BNT162b2 vaccine-induced sera against SARS-CoV-2 B.1.1.7 pseudoviruses and finds equivalent neutralizing titers compared to the Wuhan strain.

Immunoengineered Nanobead System for the Isolation and Detection of Circulating Tumor Cells

This paper develops an immuno-fluorescent magnetic nanobead system for capturing, isolating, and detecting circulating tumor cells with high efficiency and viability.

Chicken peripheral blood lymphocyte response to ALV-J infection assessed by single-cell RNA sequencing

This study uses single-cell RNA sequencing to analyze chicken peripheral blood lymphocytes infected with ALV-J, identifying T cell subsets and their molecular responses to infection.

Cas9-cleavage sequences in minimal plasmids enhance non-viral genome targeting of CARs in primary human T cells

This paper develops a non-viral method for efficient T cell genome editing using modified plasmids, achieving CAR-T cell functionality comparable to AAV-based methods.

Precise genomic deletions using paired prime editing

This paper introduces PRIME-Del, a prime editing method for precise genomic deletions, showing higher precision than CRISPR/Cas9 and enabling deletions with non-PAM junctions.

A human disease model of SARS-CoV-2-induced lung injury and immune responses with a microengineered organ chip

This paper develops a microengineered lung organ chip model to study SARS-CoV-2-induced lung injury and immune responses, revealing distinct cellular responses and the role of immune cells in inflammation.

The N-glycosylation sites and Glycan-binding ability of S-protein in SARS-CoV-2 Coronavirus

This study investigates N-glycosylation sites and glycan-binding ability of SARS-CoV-2 S-protein through phylogenetic analysis, structural comparison, docking analysis, and molecular dynamics simulation.

Inhibition of GFAT1 in lung cancer cells destabilizes PD-L1 protein and improves immune response

This study shows that inhibiting GFAT1 reduces PD-L1 stability in lung cancer cells, enhancing immune response by boosting T cell activation and NK cell cytotoxicity.

LACCASE is Necessary for Root Development in Salvia miltiorrhiza

This study shows that laccase genes are essential for root development and phenolic acid metabolism in Salvia miltiorrhiza using CRISPR/Cas9 gene knockout.

Genome-wide identification and transcriptional analyses of the R2R3-MYB gene family in wheat

The paper identifies 997 MYB genes in wheat, focusing on 445 R2R3-MYBs, and analyzes their expression patterns across 61 tissues and time points, revealing subgroup conservation and duplication mechanisms.