W. Yin

Decoration of Burkholderia Hcp1 protein to virus-like particles as a vaccine delivery platform.

This study conjugates Hcp1 protein to P22 VLPs to create a vaccine platform, inducing antibody responses and enhancing macrophage phagocytosis in mice.

A split crRNA with CRISPR-Cas12a enables highly sensitive, selective, and multiplexed detection of RNA and DNA

This paper develops a split CRISPR-Cas12a system for sensitive, selective, and multiplexed detection of RNA and DNA without reverse transcription, achieving low detection limits and improved specificity.

Human microbiota is a reservoir of SARS-CoV-2 advantageous mutations

The paper identifies that the human microbiota contributes to SARS-CoV-2 mutations by replicating bacterial mRNAs with advantageous mutations, leading to chimeric RNAs and altered viral properties.

Bio-inspired mineralization collagen induce fibrocartilage regeneration after tendon-bone injury by activating Gli1+Dkk3+ progenitor cells

This study develops a bio-inspired hydrogel that promotes tendon-bone interface regeneration by activating Gli1+Dkk3+ progenitor cells, achieving 68% mechanical recovery.

Host-microbiota interactions contributing to the heterogeneous tumor microenvironment in colorectal cancer

The paper identifies host-microbiota interactions in colorectal cancer subtypes, revealing distinct gene-microbe associations that contribute to tumor microenvironment heterogeneity.

Spatial transcriptomic analysis of virtual prostate biopsy reveals confounding effect of heterogeneity on genomic signature scoring

The paper uses spatial transcriptomic data to show that bulk genomic signature scoring in prostate cancer biopsies is confounded by tumour heterogeneity, leading to inaccurate risk assessments.

Structure of a G protein-coupled receptor with GRK2 and a biased ligand

The paper reports the structure of NTSR1 bound to GRK2, Gq, and a biased ligand, revealing how the ligand enhances GRK2 binding and enables arrestin-biased signaling.

Molecular Recognition of Itching Neuropeptides by Bombesin Receptors.

The paper presents cryo-EM structures of bombesin receptors bound to their neuropeptide ligands, revealing the mechanism of ligand selectivity and receptor activation for itch treatment.

Structural Genomics of the Human Dopamine Receptor System

This study reports cryo-EM structures of all human dopamine receptor subtypes bound to Rotigotine, revealing binding modes, activation mechanisms, and G-protein coupling specificity for drug design.

Rational identification of potent and broad sarbecovirus-neutralizing antibody cocktails from SARS convalescents

The paper identifies a potent and broad sarbecovirus-neutralizing antibody cocktail, SA55+SA58, from SARS convalescents to combat Omicron variants and other fast-evolving viruses.

Nardilysin-Regulated Scission Mechanism Activates Polo-like Kinase 3 to Suppress the Development of Pancreatic Cancer

The paper identifies a post-translational mechanism where Nardilysin cleaves Plk3 to generate an active form that suppresses pancreatic cancer development and metastasis.

E6AP is important for HPV E6 role in regulating epithelial homeostasis and its loss impairs keratinocyte commitment to differentiation

The paper shows E6AP is critical for HPV E6 regulation of epithelial homeostasis, with its loss impairing keratinocyte differentiation and YAP activation.

Biosynthetic diversification of peptaibol mediates fungus-mycohost interactions

The paper investigates how peptaibol structural diversity in Trichoderma fungi mediates interactions with mycohosts, showing that these compounds are essential for inhibiting saprotrophic fungi.

Structural and biochemical mechanism for increased infectivity and immune evasion of Omicron BA.1 and BA.2 variants and their mouse origins

This study investigates the structural and biochemical features of Omicron BA.1 and BA.2 variants, showing increased infectivity and mouse ACE2 binding compared to wildtype, suggesting a potential human-cat-mouse-human transmission cycle.

Rational design of Cas9 ribonucleoprotein with a "gRNA-shRNA" for multidimensional genome manipulation and enhanced homology-directed repair

This paper develops a Cas9-RNAi RNP tool that combines RNAi and CRISPR for simultaneous gene knock out and knock down, and enhances homology-directed repair.

Structural insights into the peptide selectivity and activation of human neuromedin U receptors

This study reports cryo-EM structures of NMUR1 and NMUR2 bound to NMU and NMS, revealing peptide selectivity mechanisms and activation principles for drug design.

Structures of the Omicron spike trimer with ACE2 and an anti-Omicron antibody: mechanisms for the high infectivity, immune evasion and antibody drug discovery

Protein and Lipid Mass Concentration Measurement in Tissues by Stimulated Raman Scattering Microscopy

The paper introduces NoRI, a SRS microscopy method to measure protein, lipid, and water concentrations in tissues, showing cell-specific variations and disease-related changes in Alzheimer's models.

Untangling Microbiota Diversity and Assembly Patterns in the World's Largest Water Diversion Canal

The paper investigates microbiota dynamics and assembly patterns in a large water diversion canal, finding deterministic processes shape bacterial communities while stochasticity dominates micro-eukaryotic communities.

Structural mechanism of calcium-mediated hormone recognition and Gβ interaction by the human melanocortin-1 receptor

The paper presents cryo-EM structures of MC1R-Gs complexes with ligands, revealing calcium's role in hormone recognition and Gβ interactions, and a conserved activation mechanism.

Molecular recognition of an acyl-peptide hormone and activation of ghrelin receptor

The paper presents cryo-EM structures of the ghrelin receptor bound to ghrelin and GHRP-6, revealing the binding pocket for the octanoyl group and providing insights for drug design.

Molecular basis for kinin selectivity and activation of the human bradykinin receptors

The paper reports structures of bradykinin receptors bound to their ligands and reveals mechanisms of selectivity and activation, providing insights for drug design.

Structural basis for ligand recognition and G protein-coupling promiscuity of the cholecystokinin A receptor

The paper presents cryo-EM structures of CCKAR with different G proteins to elucidate its G protein coupling promiscuity and ligand recognition mechanism.

Molecular basis of ligand recognition and activation of human V2 vasopressin receptor

The paper determines the structure of the V2 vasopressin receptor bound to AVP and Gs, revealing how AVP activates the receptor and providing insights for drug design.

Cas9-cleavage sequences in minimal plasmids enhance non-viral genome targeting of CARs in primary human T cells

This paper develops a non-viral method for efficient T cell genome editing using modified plasmids, achieving CAR-T cell functionality comparable to AAV-based methods.

Cell-type apoptosis in lung during SARS-CoV-2 infection

This study investigates cell-type apoptosis in lung tissues during SARS-CoV-2 infection, finding both intrinsic and extrinsic apoptotic pathways are activated, while some cell types remain refractory to infection.

Structural basis for repurpose and design of nucleoside drugs for treating COVID-19

The paper reports cryo-EM structures of SARS-CoV-2 RdRp with nucleoside inhibitors, revealing their mechanism and guiding the design of more potent derivatives.

Regulations of mitochondrial DNA repair by poly(ADP-Ribose) Polymerase 1

This study shows PARP1 regulates Pol γ activity during mtBER in an NAD+-dependent manner, with implications for drug toxicity in cardiac treatments.

Wide field-of-view volumetric imaging by a mesoscopic scanning oblique plane microscopy with switchable objective lens

This paper introduces a new variant of oblique plane microscopy (OPM) that achieves a large mesoscopic field-of-view for volumetric imaging, demonstrating its application on zebrafish larvae, mouse cortex, and C. elegans.

CD209L/L-SIGN and CD209/DC-SIGN act as receptors for SARS-CoV-2

This study identifies CD209L/L-SIGN and CD209/DC-SIGN as alternative receptors for SARS-CoV-2, capable of mediating virus entry into human cells, particularly in tissues with low ACE2 expression.