proteomics

Investigating the pathogenicity of missense spliceogenic variants using whole-genome sequencing, RNA-sequencing, and proteomics data from population and clinical research cohorts

The paper investigates missense spliceogenic variants (MSVs) using genomic and proteomic data, finding they are common and can cause disease through splicing disruption, often missed by protein-centric tools.

When can site-specific glycoform compositions resolve biosynthetic rates?

This paper evaluates when site-specific glycoform compositions can resolve biosynthetic rates, showing that sufficient panel size, separable responses, and model fit are required for accurate inference.

p53 activity links chromosomal instability to pro-migratory extracellular vesicles

The paper shows that chromosomal instability (CIN) leads to distinct p21-expressing cell populations with different cell-cycle recovery and extracellular vesicle (EV) properties, resulting in increased migration.

Identification of active deubiquitinases in dendritic cells under unstimulated and activated conditions.

The paper profiles deubiquitinase (DUB) activity and abundance in dendritic cells under unstimulated and CpG-stimulated conditions using activity-based probes and proteomics.

ASC specks and α-synuclein amplify microglial NLRP3 inflammasome activation and co-localize with Lewy body pathology

This study shows ASC-Syn assemblies enhance NLRP3 inflammasome activation in microglia and co-localize with Lewy body pathology in human brain tissue.

A 21-Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9-Color T-Cell Functional Assay

Exploratory Proteomics of Brain Extracellular Vesicle Enriched Preparations After LPS Challenge and miRNA Associated Vesicle Administration

The paper uses proteomics to study protein changes in brain extracellular vesicles after LPS challenge and miRNA treatment in rats, identifying specific protein responses and potential markers.

Intrastriatal injection of amplified Lewy body dementia (LBD) fibrils in mice results in accumulation of fibrils with LBD structure and neurodegeneration

The paper shows that injecting amplified Lewy body dementia fibrils into mice leads to fibril accumulation with LBD structure and neurodegeneration.

Reversion to the Meta Body Form of Life after Complete Cell Death in Pure Liquid Water

This study shows that after complete cell death of Staphylococcus aureus in pure water, lipid bilayer microspheres containing RNA and proteins form and exhibit dynamic stability, suggesting a simpler form of life called the Meta Body.

Plakoglobin supports length-dependent force generation and cardiac resistance to fasting

This study shows plakoglobin supports force generation in skeletal muscle and protects cardiac muscle from fasting-induced atrophy by maintaining anabolic signaling and sarcolemmal complexes.

Root exudates enhance the ecological fitness of a non-native PGPR and reshape rhizosphere microbiome assembly

This study shows how root exudates enhance a non-native PGPR's ability to thrive in the rhizosphere and alter microbial community composition.

Food Ration Affects mRNA Processing, Translation, Proteostasis, and Cytoskeletal Responses During Heat Shock in Mytilus californianus

This study shows how food ration interacts with temperature to alter proteomic responses to heat stress in mussels, affecting mRNA processing, translation, and cytoskeletal stability.

Tumour-derived factors prime microglia for immune checkpoint-mediated T-cell regulation

This study identifies an early microglial activation program driven by soluble factors from tumors, leading to immune checkpoint regulation and immunosuppression in brain tumors.

Molecular mechanism of Aurora-A binding and activation by hnRNPK

This study identifies the molecular mechanism by which hnRNPK activates Aurora-A kinase through specific residue interactions in the Y-pocket.

HSF1 inhibition potentiates the antifungal activity and proteome instability induced by the fungal Hsp110 inhibitor 2H

This study shows that combining HSF1 inhibition with Hsp110 inhibitor 2H enhances antifungal activity by increasing proteome instability in fungal pathogens.

Progranulin deficiency promotes neuroinflammation through cathepsin B-mediated activation of the NLRP3 inflammasome

This study shows that progranulin deficiency increases NLRP3 inflammasome activity via cathepsin B, leading to neuroinflammation, and demonstrates that ASO treatment can reverse this effect in GRN-deficient cells.

Large-scale exposure of southern seabirds to avian cholera Pasteurella multocida: scavengers as sentinels at different spatial scales

The paper screens seabird species for antibodies against avian cholera bacteria to assess spatial distribution and identify potential sentinels for disease monitoring.

Deciphering auditory neuroprogenitor stemness for large-scale neuronal screening

The paper characterizes the molecular and metabolic changes in auditory neuroprogenitors induced by CHIR-DS treatment and demonstrates their utility in neuronal screening.

A Framework for Comparing Mouse Neoantigen Immunogenicity

Experimental pMHC melting-temperature stability ranked in vivo CD8+ T cell responses of 25 mouse neoantigens better than affinity assays or nine in silico predictors.

ZFP36 is a post-transcriptional regulator of the P2X4 receptor in myeloid cells

The paper identifies ZFP36 as a post-transcriptional regulator of P2X4 receptor expression in myeloid cells, revealing a novel mechanism linking myeloid cell state to purinergic responsiveness.

Sexual reproduction impacts vertebrate organ aging

This study shows that lifelong mating in killifish affects liver aging in a sex-specific manner and is associated with younger transcriptomic and proteomic ages in humans.

HIV-1 envelope glycan loss links receptor adaptation and innate immune escape in SHIVs

This study shows that HIV-1 envelope glycan loss in SHIVs improves CD4 receptor utilization and reduces IFN restriction, linking receptor adaptation and immune escape.

Targeting MAT2A-S-adenosylmethionine (SAM) Axis Attenuates DNA Damage Response and Cancer Stemness to Increase Platinum Sensitivity in Ovarian Cancer

Blocking MAT2A-driven SAM synthesis in ovarian cancer cells prevents cisplatin-induced DNA hypermethylation, impairs DNA repair signaling, and blocks stem cell enrichment, sensitizing MTAP-proficient and MTAP-deficient lines.

Spatial proteomics identifies altered thick ascending limb cells as drivers of kidney disease through uromodulin retention

This study identifies altered thick ascending limb cells with UMOD retention as drivers of CKD using spatial proteomics and shows reducing UMOD retention attenuates CKD development.

Predicting cyanobacterial oxic diazotrophy and prioritizing candidate protein families beyond known Nif proteins

The paper predicts cyanobacterial diazotrophy using protein-family content and prioritizes candidate protein families for experimental validation.

NE52-QQ57 attenuates post-ischemia protein phosphorylation in the mouse brain

This study shows that NE52-QQ57 reduces post-ischemia protein phosphorylation and crosses the blood-brain barrier in mice.

Serotype-independent inhibition of S. pneumoniae by SMiTE, a commensal-derived bacteriocin

The paper identifies SMiTE, a commensal-derived bacteriocin that effectively inhibits multiple Streptococcus pneumoniae serotypes without harming commensals.

Spatiotemporal profiling of T cell repertoires in Lynch syndrome reveals convergent immune responses through neoplastic progression

This study profiles T cell repertoires in Lynch syndrome patients to show convergent immune responses during neoplastic progression.

Identification of the Col4a3-/- mouse as a model of chronic kidney disease-associated cachexia

The paper identifies the Col4a3-/- mouse as a model of CKD-associated cachexia, showing significant weight loss, muscle atrophy, and systemic inflammation.

DTCAMD, a disease-associated truncated form of CRYAB in neovascular age-related macular degeneration: a potential driver of subretinal fibrosis

This study identifies a truncated form of CRYAB, DTCAMD, as a potential therapeutic target for subretinal fibrosis in nAMD.