proteomics

APEX2 proximity labeling maps the mGBP2-proximal proteome at the Toxoplasma gondii parasitophorous vacuole

The paper uses APEX2 proximity labeling to identify mGBP2-associated proteins at the Toxoplasma gondii parasitophorous vacuole and finds COX2 is critical for parasite survival.

Anisomycin-induced ribosome collisions follow a dose-dependent positional rule, distinct from the UV-induced collision landscape

Low- and high-dose anisomycin confine ribosome collisions to a dose-shifted zone just past the start codon, whereas 30 min after UV, collisions appear more dispersed and favor highly translated mRNAs.

Cytoplasmic FKBP7 reflux sustains NFE2L1 levels in an adaptive response to chemotherapy in prostate cancer cells

This paper identifies FKBP7's role in sustaining NFE2L1 levels to promote chemoresistance in prostate cancer cells through cytoplasmic translational control.

Inhibiting 2-oxoglutarate dehydrogenase reveals the mechanisms and limits of metabolic plasticity in developing maize kernels

This study investigates the role of 2-oxoglutarate dehydrogenase in maize kernels by knocking it down and analyzing metabolic changes and structural impacts.

To Recover or Not to Recover: A Urinary Proteomic Perspective on Severe Acute Kidney Injury

This study evaluates a urinary FABP3/APOA1 ratio as a predictor of renal recovery after severe AKI, showing high accuracy in both discovery and validation cohorts.

Sparse Phosphorylatable Residues, Tuned by Agonist Chemotype, Shapes GPCR Signaling

This study shows that GPCR signaling diversity can arise from a sparse set of phosphorylation sites rather than an extensive barcode, with ligand chemotype influencing their functional impact.

Phosphorylation of Z granule components regulates germ granule organization and RNAi inheritance

This study shows that phosphorylation of Z granule components regulates germ granule organization and RNAi inheritance in C. elegans.

Oncogenic ETS factors expressed from fusion genes utilize EWSR1 to associate with the paraspeckle and regulate transcription.

This study shows that EWSR1 from fusion genes interacts with paraspeckles to regulate transcription in cancers, and disrupting paraspeckles mimics loss of oncogenic ETS factors.

Pre-chlorotic Mg2+ deficiency impairs guard-cell PSII and leaf gas exchange in Vicia faba

This study shows that magnesium deficiency impairs guard-cell photosystem II and leaf gas exchange before visible chlorosis in Vicia faba.

An ER Retrieval-and-Release System Reveals Ligand-Independent Basal Signaling by Cell Surface TCRs

The paper develops an ER retrieval-and-release system to study ligand-independent basal TCR signaling, finding that surface TCRs generate distinct phosphorylation patterns compared to ligand-induced activation.

The Human Contraceptive Antibody Targets A Semenogelin Galectin-3 N-Linked Glycan Complex on the Sperm Surface

This paper identifies the HCA antibody's target as a semenogelin-galectin-3 complex on the sperm surface, rather than CD52, through affinity purification and glycan analysis.

Bacterial vitamin sharing emerges from a balance between release and uptake

The paper quantifies vitamin B12 synthesis and uptake in bacterial isolates, revealing strategies that govern extracellular B12 availability through a balance between release and uptake.

Joint Variable Selection for Omic Biomarkers in Time-to-Event Data

The paper introduces vampW, a Bayesian method for joint variable selection in omic biomarkers and time-to-event data, showing reduced false discoveries and improved prediction.

The FERONIA receptor kinase is required for high humidity responses in Arabidopsis

This study identifies FERONIA and LLG1 as critical regulators of Arabidopsis high humidity responses, including transcriptomic changes and leaf hyponasty.

Rapid, reversible RNA/DNA precipitation using rare earth ion coordination

This paper introduces NADA and ALGO for rapid, solvent-free RNA/DNA precipitation and recovery, demonstrating their effectiveness in viral RNA and DNA handling.

Proteomics of steady-state and culture-derived human erythropoiesis reveals the dynamics of erythroid stress

This study compares proteomic profiles of steady-state and cultured human erythroid progenitors, revealing how culture-induced stress alters protein expression and erythroid maturation dynamics.

Enzyme-Linked Cycloaddition Assay (ELCA) for rapid, ultra-sensitive monitoring of secreted sialoglycoproteins

This paper introduces ELCA, a method for ultra-sensitive detection of secreted sialoglycoproteins, showing rapid response to pharmacological perturbations and compatibility with serum-containing conditions.

AKT-like kinase regulates early steps in the Trypanosoma brucei quorum sensing signalling pathway

This study identifies AKT-like kinase as a regulator of quorum sensing in Trypanosoma brucei, showing its role in differentiation and potential as a therapeutic target.

Cirrhosis-associated immune dysfunction reflects impaired intestinal CD8ab⁺ intraepithelial lymphocyte compartmentalization

The paper investigates immune dysfunction in cirrhosis by analyzing CD8ab⁺ intraepithelial lymphocytes and identifies CCL25-CCR9 axis disruption as a key factor.

Programming Targeted Immune Cell Migration Using Orthogonal Chemokine-Receptor Pairs

This study engineers orthogonal chemokine-receptor pairs to enable targeted immune cell migration and collective swarming behavior in engineered T cells.

Allosteric disulfide control of ligand binding and endocytosis of KIR2DL4, the natural killer cell receptor for HLA-G

This study identifies an allosteric disulfide bond in KIR2DL4 that regulates HLA-G binding and endocytosis, influencing NK cell function during pregnancy.

The mitochondrial transaminase GOT2 supports Japanese encephalitis virus replication in neuronal cells

This study identifies the mitochondrial transaminase GOT2 as a metabolic dependency of JEV and a potential host-directed antiviral target through integrated transcriptomics and proteomics analyses.

A cell death-independent role for MLKL in TGF-β receptor recycling in Marfan syndrome

This study identifies a non-cell death role for MLKL in TGF-β receptor recycling and aortic integrity in Marfan syndrome.

Asymptomatic colonization by Botrytis cinerea reveals polarized plant-fungus interfaces before widespread disease

This study examines asymptomatic Botrytis cinerea colonization in Arabidopsis seedlings, revealing polarized host responses and fungal morphologies before visible disease.

Separation, isolation, and membrane lipid profiling of selectively vulnerable and resilient neurons

This study develops a method to isolate and profile membrane lipids of vulnerable and resilient neurons to investigate their differential responses to oxidative stress.

Catalase import into peroxisomes in Arabidopsis

This study identifies a minimal C-terminal targeting motif for plant catalases that enables their peroxisomal import in Arabidopsis.

EF-P-resolved ribosome stalling and collision trigger Rae1-mediated mRNA decay

The paper identifies new Rae1 cleavage sites and shows that ribosome stalling and collisions trigger mRNA decay in Bacillus subtilis, with sequence context influencing cleavage efficiency.

Flavins in Focus: Vitamin B2 as a Metabolic Keystone for Neuronal Repair in Regenerating Planarians

This study identifies flavin-rich cells essential for neuronal repair in regenerating planarians, showing their role in spatially organizing vitamin B2 metabolism during tissue regeneration.

VPS34 regulation in pulmonary arterial vascular smooth muscle cells in pulmonary hypertension

This study investigates VPS34 activation in pulmonary arterial vascular smooth muscle cells in pulmonary hypertension, finding it drives proliferation and fibrotic remodeling, and shows SAR405 inhibition reduces disease progression.

Hepatocyte UBE4A protects against MASLD/MASH by maintaining ER homeostasis via UBXN4 interaction

This study identifies Ube4A's role in protecting against MASLD/MASH by maintaining ER homeostasis through its interaction with UBXN4, highlighting potential therapeutic targets.