DNA Repair Mechanisms

This study reports the first structures of a dimeric UvrD-family helicase, revealing the 2B subdomain's regulatory role in dimerization and activation.

This study identifies MDC1 as a mediator of Pellino1 and Pellino2 recruitment to DNA double-strand breaks through phosphorylation-dependent interactions.

This study investigates how the CMG helicase bypasses DNA-protein crosslinks on the leading strand without requiring additional replisome factors or unfolding the protein adduct.

This study shows NAT10 depletion affects DNA repair proteins and 3D-genome structure but not histone modifications.

This study examines how the timing of lesion bypass during DNA replication affects mutagenesis, showing that TLS at certain lesions occurs post-replicatively and competes with DA, reducing mutagenicity.

The paper shows that replication fork collapse at SSBs leads to seDSBs resolved by homologous recombination, but DNA synthesis downstream is inefficient, while deDSBs allow efficient synthesis without recombination.

This study investigates the role of MutLgamma in meiotic crossover formation in Arabidopsis thaliana, showing it is partially dispensable for ZMM-dependent crossovers and influences resolution of recombination intermediates.

This study shows that cancer cell lines exhibit dysregulated LoL-DDR, leading to increased RIR and 8-oxoG accumulation, creating a replication stress-ROS vicious circle that promotes tumorigenesis.

This study shows that rapid delivery of RNase H1 rescues replication fork progression under stress without affecting R-loops.

This study identifies that high levels of DNA replication initiation factors correlate with ATR inhibitor sensitivity due to excessive origin firing in cancer cells.

The paper identifies FOXM1 expression levels as a factor in replication stress tolerance in oncogenic RAS-expressing cells using single-cell RNA sequencing and drug combination experiments.

This study identifies AEP's role in promoting radioresistance in breast cancer by modulating the ATR pathway and PPP1R10 levels, showing AEP-high/ATR-low as a biomarker for radioresistant patients.

This study investigates the role of MutL complexes in Arabidopsis meiosis, showing that MUS81 can partially compensate for MutL{gamma} loss and that crossover interference is reduced in mlh1 mutants.

This study shows that a unique secondary contact of Pol IV with the processivity clamp enhances its ability to perform translesion synthesis past strong replication blocks.

This study shows that SPO11 dimerization is essential for meiotic DNA double-strand break formation, demonstrating that SPO11 can cleave DNA alone or with TOP6BL.

The paper develops a method to map 5'-aldehyde termini in DNA at single-nucleotide resolution, revealing a preference for adenine bases and linking these lesions to genomic features and chromatin accessibility.

This paper shows PP4Psy2 regulates DSB resection by modulating Pif1 and Rrm3 helicases, particularly in regions with tandem retrotransposons.

This study identifies evolutionary changes in Rad51 and Dmc1 that influence recombination outcomes by altering DNA binding properties and pathway stability.

This study reconstitutes SPO11-dependent DNA double-strand break formation in vitro, revealing dimerization-dependent cleavage and topoisomerase activity.

This study shows that PCH-2 antagonizes crossover formation in C. elegans meiosis, controlling crossover number and distribution through interactions with HORMADs.

This study identifies the CHAMP1 complex's role in heterochromatin assembly and its enhancement of homology-directed DNA repair in specific genomic regions.

This study shows SET8 inhibits BRCA1 signaling during S-phase by promoting 53BP1 accumulation, and its degradation is essential for BRCA1 and RAD51 complex nuclear focal accumulation.

This study shows that TRAIP depletion causes PCNA retention on chromatin during mitosis, leading to reduced Cdt1 levels and impaired S phase entry.

This study reveals how cohesin facilitates homology search during DNA repair by reshaping chromosome architecture through two distinct cohesin pools.

This study investigates the role of H2B mono-ubiquitylation in homologous recombination repair by analyzing its effects on D-loop metabolism and chromatin dynamics in S. cerevisiae.

This study shows that XRCC1 facilitates PARP1-mediated recruitment of PARP2 to DNA damage sites through PAR binding and interaction with PARP2.

This study investigates how UV radiation induces reorganization of the 3D genome and its role in DNA damage response and repair.

The paper identifies a conserved, disordered domain in SYP-4 that regulates crossover assurance and interference in meiosis by recruiting an E3 ligase.

The paper uses proteomics to analyze protein dynamics during replication origin firing in human cells, identifying NCAPH2 as a novel factor required for efficient origin firing.

The paper identifies RAD54L2 as a regulator of recombination by showing its interaction with the BTRR complex and its role in suppressing sister chromatid exchanges.