Microtubule and mitosis dynamics

CDK1-dependent N-terminal NuMA phosphorylation promotes dynein-dynactin-NuMA assembly for accurate chromosome segregation

This study shows CDK1-dependent phosphorylation of NuMA's N-terminus is essential for dynein-dynactin-NuMA complex formation, ensuring accurate chromosome segregation.

TPGS1 Regulates Central Spindle Microtubule Glutamylation and Remodeling During Telophase and Abscission

This study shows TPGS1 regulates polyglutamylation and remodeling of central spindle microtubules during telophase, affecting mitotic cell division completion.

Microtubule end stabilisation by cooperative oligomers of Ska and Ndc80 complexes

The paper shows that Ska and Ndc80 complexes form oligomers to stabilize microtubule ends during mitosis, with a mutation disrupting Ska:Ska interactions and impairing stability.

Tau-Driven Coordination of Microtubule-Actin Crosstalk in Cell-Sized Vesicles

The paper investigates how tau protein coordinates microtubule-actin interactions in synthetic cell-like vesicles, showing its role in MT organization and MT-actin colocalization depending on actin crosslinkers and vesicle size.

Cep192 insufficiency underlies haploid instability in human cells

This study identifies Cep192 insufficiency as a cause of haploid instability in human cells and shows that restoring Cep192 or enhancing the acentrosomal spindle pathway stabilizes haploid cells.

Optogenetically Induced Microtubule Acetylation Unveils the Molecular Dynamics of Actin-Microtubule Crosstalk in Directed Cell Migration

This study uses optogenetics to induce microtubule acetylation and reveals its role in enhancing actomyosin contractility and directed cell migration through GEF-H1 release.

A biochemical probe for microtubule lattice integrity uncovers motor-caused lattice damage

This paper introduces MT-DS, a fluorescent probe that directly visualizes microtubule lattice damage, revealing motor-caused damage during motility.

Intellectual disability-causing mutations in KIF11 impair microtubule dynamics and dendritic arborization

This study shows that KIF11 mutations impair microtubule dynamics and dendritic arborization in neurons, contributing to intellectual disabilities.

Multi-omic analyses reveal a differential contribution of chromatin-associated PP1 holoenzymes to mitotic exit and G1 re-establishment.

The paper investigates the role of three chromatin-associated PP1 targeting subunits in mitotic exit and G1 re-establishment using multi-omic analyses.

Fidelity-Ensuring Consistency of Mitosis is Safeguarded by the 53BP1-USP28-p53 Pathway

The paper identifies the 53BP1-USP28-p53 pathway as an external mitotic surveillance system that induces post-mitotic arrest when fidelity-ensuring mechanisms are compromised.

Polar chromosomes are rescued from missegregation by spindle elongation-driven microtubule pivoting

The paper shows that spindle elongation rescues polar chromosomes from missegregation by pivoting astral microtubules toward the spindle surface, with implications for chromosomal instability in cancer.

Activation and regulation of the dynein-dynactin-NuMA complex

This study investigates how NuMA activates dynein and regulates microtubule dynamics during mitosis using structural and functional analyses.

Spheroid culture remodels mitosis and the proteome in tumor cells

This study compares mitosis in 3D tumor spheroids and 2D monolayers, finding prometaphase delays and proteomic changes in spheroids.

Contributions of error correction and the spindle assembly checkpoint to mitotic timing and fidelity

This study develops a model to analyze how error correction and the spindle assembly checkpoint affect mitotic timing and fidelity in human cells.

A pivotal contribution of proteostasis failure and mitochondrial dysfunction to chromosomal instability-induced microcephaly

This study identifies proteostasis failure and mitochondrial dysfunction as key factors in microcephaly caused by complex aneuploidies in neural stem cells.

Kinesin-induced buckling reveals the limits of microtubule self-repair

This study shows that kinesin-driven microtubule buckling causes severe lattice damage that often exceeds microtubule self-repair capacity, leading to breakage, but survival is enhanced with intracellular factors.

The microtubule GTP-tubulin cap size is modulated during cell division

The paper uses live-cell imaging to show that GTP-cap size decreases during mitosis, modulating microtubule stability in a cell-cycle-dependent manner.

A minimal model for the role of Rim4 in regulating meiotic exit in budding yeast

The paper develops a mathematical model to show that Rim4 degradation and Ama1 translation are essential for irreversible meiotic exit in budding yeast.

Zebrafish otic vesicle and mouse epididymis as model systems for studying columnar epithelial cell division

The paper uses zebrafish otic vesicles and mouse epididymis to study columnar epithelial cell division dynamics, revealing conserved and species-specific mechanisms of nuclear migration and division.

Dimeric Cin8 motors have an inherent plus-end bias and weak inter-head coordination

This study investigates the bidirectional motility of Cin8 kinesin-5 dimers, finding a plus-end bias and weak inter-head coordination, suggesting directional switching is regulated by coordination levels.

The dynamics of centromere assembly and disassembly during quiescence

The paper examines centromere protein dynamics during quiescence entry and exit, showing rapid disassembly and reassembly of centromere proteins with specific timing for CENP-A deposition.

Wnt signalling controls abscission dynamics in mouse embryonic stem cells

This study shows that Wnt signaling controls abscission dynamics in mouse embryonic stem cells by regulating Aurora B activity and microtubule stability at the intercellular bridge.

Expression of recombinant human glutamylating TTLLs in human cells leads to differential tubulin glutamylation patterns, with only TTLL6 disrupting microtubule dynamics

This study shows that TTLL6 disrupts microtubule dynamics and that LDC10 inhibits TTLL activity and reverses TTLL6's effects.

Septin Complexes Regulate Microtubule Organization and Synaptic Function at the Neuromuscular Junction

This study shows that septin complexes in Drosophila regulate microtubule organization and synaptic function at neuromuscular junctions by stabilizing microtubules and affecting vesicle recycling.

Cargo recognition of Nesprin-2 by the dynein adapter Bicaudal D2 for a nuclear positioning pathway that is important for brain development

The paper identifies the molecular mechanism by which Nesprin-2 interacts with Bicaudal D2 to recruit dynein for nuclear positioning during brain development.

Shugoshin holds the potential to inhibit APC/C and thereby prevents separase activation

This study shows that Sgo1 inhibits APC/C to prevent separase activation during mitosis but enhances Rec8 cohesin protection during meiosis through its D-box.

Cell confinement initiates a delayed but heritable loss of chromosomes

The paper shows that cell confinement causes delayed, heritable chromosome loss, which is linked to mitotic memory and has implications for cancer evolution.

Assembly and phospho-regulatory mechanisms of the budding yeast outer kinetochore KMN complex

This study uses cryo-EM to reveal structural mechanisms of KMN complex assembly and phospho-regulation in budding yeast kinetochores.

Activated kinesin-1 assembles into a dimer-of-dimers

This study shows that neuronal kinesin-1 (KIF5C) forms tetramers through dimer-dimer association, with conformational activation promoting this higher-order assembly.

Plasmodium ARK1 regulates spindle formation during atypical mitosis and forms a divergent chromosomal passenger complex

This study identifies ARK1 as a critical regulator of mitosis in Plasmodium, forming a non-canonical chromosomal passenger complex with divergent INCENPs, and highlights its potential as an antimalarial target.