Microtubule and mitosis dynamics

Analyses of bent spindles reveal the mechanics of anaphase B in fission yeast

The paper investigates the mechanical properties of fission yeast spindles by analyzing their response to mechanical and genetic perturbations, revealing their behavior as a rigid beam and identifying key proteins involved in elongation and structural integrity.

Discovery and in vivo characterization of novel TOG domain-containing proteins using C. elegans

This study identifies and characterizes two novel TOG domain-containing proteins in C. elegans, showing their role in sperm function and microtubule regulation.

WDR62 and CEP170 recruit MAPKBP1 for pericentriolar material cohesion and mitotic spindle formation.

This study identifies MAPKBP1 as a centrosomal protein that forms a complex with WDR62 and CEP170, essential for PCM cohesion and mitotic spindle formation.

Microtubule lattice defects facilitate spastin-mediated severing

This study shows that microtubule lattice defects accelerate spastin-mediated severing by reducing the number of tubulin subunits needed for cleavage, without preferential spastin binding to defects.

Auxin-inducible degradation of UNC-116 in C. elegans inhibits bidirectional dense core vesicle transport and worm locomotion on different timescales

This study depletes UNC-116 in C. elegans using auxin-mediated degradation and shows its role in bidirectional DCV transport and locomotion across different timescales.

A unified model of aneuploid karyotype dynamics

This study develops a computational model of aneuploid karyotype dynamics in budding yeast, revealing that reversion mechanisms vary by chromosome and can involve coupled nondisjunction and recombination.

DYN-1 regulates SPD-2 and PLK-1 localization and mitotic spindle pole organization

This study identifies DYN-1's role in regulating spindle pole organization and midbody assembly in C. elegans by controlling SPD-2 and PLK-1 localization.

Role of α-tubulin helix 11' in heterodimer conformation and microtubule dynamics

The paper investigates the role of α-tubulin helix 11 in tubulin heterodimer conformation and microtubule dynamics, showing its impact on disease and evolutionary conservation.

GSK-3β coordinates axonal microtubule organisation through Shot and Tau

This study shows that GSK-3β regulates axonal microtubule organisation via Shot and Tau, and misregulation causes microtubule disorganisation linked to neurodegeneration.

Tissue mechanics and systemic signaling safeguard epithelial tissue against spindle misorientation.

The paper investigates how epithelial tissues maintain architecture and cell number despite spindle misorientation, identifying mechanical and systemic signaling mechanisms that prevent cell loss.

Asgard archaeal origin of microtubules

The paper identifies tubulins in Asgard archaea and shows they form mini-microtubules with eukaryotic-like properties, suggesting an archaeal origin for microtubules.

Tubulin acetylation governs organelle remodeling and lysosomal reformation during neuronal differentiation

This study shows that tubulin acetylation regulates lysosomal reformation and organelle interactions during neuronal differentiation by shaping organelle architecture and spatial distribution.

Viral rewiring of APC/C-CDC20 drives Aurora B hyperubiquitination, mitotic regression and polyploidy

This study shows that adenovirus E4orf4 interacts with CDC20 to redirect APC/C-CDC20 towards Aurora B, causing hyperubiquitination and mitotic regression, leading to polyploidy.

Removal of nascent transcripts by TTF2 is required for sister chromatid resolution in human cells.

This study shows that TTF2 removal causes mitotic defects by retaining transcription on chromatin, leading to sister chromatid resolution failure and genome instability.

Lattice Instability Drives Formation of Protofilament Clusters at the Microtubule Plus End Tips

This paper develops a coarse-grained model to study protofilament clustering at microtubule plus-end tips, showing lattice instability drives PF cluster formation and dynamics.

Chromosomal instability and chromosome 17p loss drive convergentNDE1 synthetic lethality in metastatic cancer cells

The paper identifies NDE1 as a synthetic lethal target in metastatic cancer cells driven by chromosomal instability and Chr17p loss.

To Be or Not to Be an Oocyte: Msps/XMAP215 Controls Oocyte Cell Fate in the Drosophila Ovary

This study shows Msps/XMAP215 promotes oocyte fate by enhancing microtubule polymerization and Orb accumulation, leading to asymmetric distribution and positive feedback in Drosophila.

How the non-motile kinesin KIF7 adapts conserved kinesin principles for itsfunction in Hedgehog signaling

This study investigates how KIF7, a non-motile kinesin, uses conserved kinesin principles to function as a scaffold in Hedgehog signaling by regulating GLI transcription factors.

Conditional Localization Pharmacology Manipulates Cell Cycle with Spatiotemporal Precision

This paper introduces a method to control kinase activity with spatiotemporal precision using conditional localization pharmacology, demonstrating its ability to manipulate the cell cycle.

Coordinated control of both microtubule ends regulates mitotic spindle length

This study resolves a paradox in spindle length control by showing that coordinated regulation at both microtubule ends allows spindle length and poleward flux to vary independently.

Accessibility of the unstructured α-tubulin C-terminal tail is controlled by microtubule lattice conformation

The paper investigates the accessibility of the α-tubulin C-terminal tail (Y-CTT) and finds it is regulated by microtubule lattice conformation through nucleotide state and MAP interactions.

Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo

This study investigates the conformational flexibility of kinesin-1 using biophysical methods, revealing its open structure without cargo and insights into autoinhibition.

M18BP1 valency and a distributed interaction footprint determine epigenetic centromere specification in humans

The paper identifies M18BP1 dimerization and its interaction determinants as key for centromere specification through epigenetic mechanisms in humans.

Countervailing effects of cyclin isoforms A and B during mitosis

This study shows Cyclin A2 and B1/B2 have opposing roles in regulating kinetochore dynamics and mitotic progression in human cells.

Molecular motors orchestrate pause-and-run dynamics to facilitate intracellular transport

The paper investigates intracellular transport pauses, finding they are active, motor-driven states rather than passive, and reveals a quantitative relationship between pause diffusivity and run velocity.

Microtubule depolymerization at kinetochores restricts anaphase spindle elongation

The paper shows that microtubule depolymerization at kinetochores limits spindle elongation rather than moving chromosomes apart during anaphase.

The Chromatin Protein CFDP1 Activates TPX2 and Promotes Chromosomal Microtubule Nucleation and Spindle Assembly

This study identifies CFDP1 as a bipartite MAP that promotes spindle assembly by interacting with TPX2 and Aurora A, and facilitating importin dissociation from TPX2.

Molecular basis for the activation of Aurora A and Plk1 kinases during mitotic entry

This paper identifies how Bora activates Aurora A and Plk1 kinases during mitosis through structural and biochemical analysis.

Molecular requirements for PLK1 activation by T-loop phosphorylation

This study identifies Bora as essential for Aurora A-mediated phosphorylation of PLK1's Thr210, revealing a unique interaction required for this process.

Bora Bridges Aurora-A Activation and Substrate Recognition of PLK1

This study presents structural models of the Aurora-A/Bora and Aurora-A/Bora/PLK1 complexes, revealing how Bora bridges Aurora-A activation and PLK1 substrate recognition.