Microtubule and mitosis dynamics

Tubulin Acetylation Deficiency Promotes Axonemal Turnover and Increases Cytoplasmic Microtubules

The paper shows that tubulin acetylation deficiency increases axonemal turnover and cytoplasmic microtubule dynamics without affecting flagellar length or cell growth.

Sister chromatid separation determines the proliferative properties upon whole-genome duplication via homologous chromosome arrangement

This study shows that mitotic slippage and cytokinesis failure, two mechanisms of whole-genome duplication, differentially affect post-WGD cell viability through distinct chromosome segregation patterns.

MATCAP1 preferentially binds an expanded tubulin conformation to generate detyrosinated and ?C2 a-tubulin

This study investigates how MATCAP1 binds to expanded microtubule conformations to generate detyrosinated and ΔC2 α-tubulin modifications.

Outer Kinetochore Proteins form Linear Elements to Regulate Vesicle Transport

The paper identifies linear elements formed by outer kinetochore proteins that regulate vesicle transport during anaphase I in C. elegans oocytes.

The gamma-Tubulin Ring Complex promotes mitotic spindle integrity and acts as a microtubule minus-end cap during mitosis

This study shows that gamma-Tubulin Ring Complex (gamma-TuRC) is essential for mitotic spindle integrity, acting both as a nucleator and a minus-end cap.

In vitro reconstitution defines the mechanistic basis of HSET motor activity regulation by IntraFlagellar Transport proteins

This study uses an in vitro reconstitution system to show that IFT proteins enhance HSET kinesin processivity, promoting microtubule sliding and centrosome clustering.

Midzone bundles of the mammalian anaphase spindle are mechanically coupled both locally and globally

The paper uses microneedle manipulation to show that anaphase spindle midzone bundles transmit force both locally and globally, revealing their mechanical coupling and role in chromosome segregation.

Liquid-solid phase transitions in the biological condensates of a conserved mitotic spindle regulator

This study investigates phase transitions in biological condensates formed by the mitotic spindle regulator Mud and related proteins, revealing liquid-to-solid transitions and their molecular mechanisms.

Tubulin polyglutamylation modulates Golgi morphodynamics and neurite branching during neuronal morphogenesis

This study shows that tubulin polyglutamylation affects Golgi structure and neurite branching during neuronal development.

Specialisation of meiotic kinetochores revealed through a synthetic spindle assembly checkpoint strategy

The paper introduces a synthetic SAC strategy to study meiosis II in yeast, revealing differences in kinetochore composition and phosphorylation between meiotic metaphase I and II.

Interaction of IRS2 with PLK1 protects cells from mitotic stress

This study identifies IRS2's role in protecting cells from mitotic stress through its interaction with PLK1, distinct from its signaling function.

Cytokinetic abscission failures in a polarized epithelium affect apical membrane size and cilia

This study examines how abscission failures in a polarized epithelium affect apical membrane size and cilia, using Cep55 knockout mouse neuroepithelium as a model.

Selective autophagy of whole micronuclei suppresses chromosomal instability

The paper shows that autophagy selectively degrades micronuclei with nuclear envelope defects, preventing chromosomal instability by eliminating missegregated chromosomal material.

Proteomics reveals extensive phosphoregulation of outer kinetochore protein KNL1

The paper identifies 111 phosphorylation sites on KNL1, showing extensive phosphoregulation in response to microtubule-disrupting compounds.

Spatiotemporal organization of dynein in bulk cytoplasm promotes aster growth and positioning in large embryos

The paper investigates how dynein organization in bulk cytoplasm coordinates aster growth and centrosome positioning during cleavage in large embryos.

Polo-like kinase Cdc5 orchestrates Cdk1 regulation via Swe1 and Mih1 during meiotic prophase I exit

The paper shows Cdc5 promotes Cdk1 activation during meiotic prophase I exit by inducing Swe1 degradation and facilitating Mih1 nuclear translocation, revealing a novel role for Cdc5 in meiotic progression.

The cytoplasmic lattice in mammalian eggs sequesters ubiquitination machinery and tubulin in reserve

This study reveals the structure and function of the cytoplasmic lattice in mammalian eggs, showing it sequesters ubiquitination machinery and tubulin for use during early embryonic development.

Meiotic cohesin paralogs govern cell survival by exhibiting flexibility in partner choice

This study shows meiotic cohesin paralogs can form hybrid complexes with mitotic subunits in somatic cells, affecting cell survival and genomic stability.

Kinase-gated coincidence detection controls kinesin-driven lysosome transport

The paper identifies a phosphorylation-dependent gate on kinesin-1 activity mediated by KLC2 CTD, revealing a kinase-kinase code that regulates lysosome transport through adaptor binding and membrane cues.

Mitotic errors drive rapid clearance of polyploidy during intestinal regeneration despite robust centrosome clustering

The paper shows that polyploid cells in intestinal regeneration are cleared through mitotic errors despite centrosome clustering, leading to rapid loss of faulty lineages.

KATANIN promotes cell elongation and division to generate proper cell numbers in maize organs

This study shows that KATANIN, encoded by two genes in maize, promotes cell elongation and division by affecting microtubule severing, PPB formation, and nuclear positioning, leading to smaller and abnormally shaped mutant plants.

Curcumin delays cytokinesis in fission yeast cells by targeting the septin ring

This paper shows curcumin targets the septin ring in fission yeast, delaying cytokinesis and forming a fluorescent ring that co-localizes with septins and the exocyst complex.

Centriolar satellites regulate CEP350 mRNA localization and centrosome amplification

This study identifies a pathway involving centriolar satellite proteins and an RNA binding protein that regulate CEP350 mRNA localization to centrosomes and its role in centrosome amplification in breast cancer cells.

Regulation of microtubule abundance and minus end dynamics by Katanin, CAMSAPs, WDR47 and kinesin-13

This study investigates how Katanin, CAMSAPs, WDR47, and kinesin-13 regulate microtubule abundance and minus-end dynamics through in vitro reconstitution.

Spindle pole proteins confine chromosomes to ensure their expulsion during female meiosis

The paper identifies ZYG-9/ch-TOG as a spindle pole protein that binds DNA and packages meiotic chromosomes into polar bodies, ensuring proper genome copy number in oocytes.

SMAD4 loss drives chromosomal instability during tumourigenesis via translational reprogramming

This study shows SMAD4 loss reprograms translation to induce chromosomal instability, driving tumourigenesis, and identifies CDK11B-p58 as a key mediator.

Distinct D-Box Motifs in SPD-2 Mediate APC/CFZR-1-Dependent Degradation and Centrosomal Localization in Caenorhabditis elegans

The paper identifies D-box motifs in SPD-2 that mediate APC/CFZR-1-dependent degradation and centrosomal localization in C. elegans, revealing their dual role in regulating centrosome assembly.

FTO regulates centrosome function and mitotic fidelity in cancers with chromosome instability (CIN)

This study shows FTO regulates centrosome function and mitotic fidelity, with CIN cancers being more sensitive to FTO inhibition.

Neck-region-microtubule interactions direct counterclockwise stepping of kinesin-1

This study uses molecular dynamics simulations to show that neck-region interactions with microtubules direct counterclockwise stepping in kinesin-1.

lncRNA NORM is essential for proper chromosome segregation through the Plk1-Bub1 and Nsun2 axis.

This study shows that lncRNA NORM is essential for proper chromosome segregation by regulating the Plk1-Bub1 and Nsun2 axis during mitosis.