Microtubule and mitosis dynamics

Yeast two-hybrid screening identifies Dad1 as a candidate substrate of Hrr25 kinase in Saccharomyces cerevisiae

The paper identifies Dad1 as a potential substrate of Hrr25 kinase in yeast through yeast two-hybrid screening and structural analysis.

Membrane rupture and independent extension of sister membranes drive cytokinesis in C. elegans embryos

This study reveals that cytokinesis in C. elegans embryos occurs through membrane rupture and independent extension of sister membranes, challenging the classical model of continuous membrane invagination.

hSpindly's dynamic controls SAC activity independently of the KBB pathway at unattached kinetochores

This study identifies hSpindly as a dynamic modulator of SAC activity via the RZZ pathway, independent of the KBB pathway, and highlights its role in drug resistance.

The A-C Linker controls centriole cohesion and duplication

The paper identifies A-C linker proteins and shows their role in centriole cohesion, duplication, and structure.

The microtubule-severing enzyme spastin regulates spindle dynamics to promote chromosome segregation in Trypanosoma brucei

This study identifies spastin's role in regulating spindle dynamics and chromosome segregation during mitosis in Trypanosoma brucei.

The Unkempt RNA binding protein reveals a local translation program in centriole overduplication

The paper identifies Unkempt as a key RNA-binding protein promoting local translation at centrosomes during Plk4-induced centriole overduplication.

Multi-kinesin clusters impart mechanical stress that reveals mechanisms of microtubule breakage in cells

The paper investigates how multi-kinesin clusters induce mechanical stress leading to microtubule breakage, estimating a lower rupture force than previous in vitro studies.

Cooperative multivalency converts disorder into rods, resolving a paradox in cellular architecture

The paper shows how LC8 converts the disordered KANK1 linker into a rod-like structure through cooperative multivalency, resolving a cellular architecture paradox.

Calyculin A Induces Premature Chromosome Condensation and Chromatin Compaction in G1-Phase HeLa Cells without Histone H1 Phosphorylation

Regulation of X. laevis M18BP1 centromeric localization and CENP-A assembly

This study identifies a cell cycle-dependent phosphorylation mechanism that regulates M18BP1 binding to CENP-A nucleosomes, controlling CENP-A assembly during interphase.

A pivot-tether model for nucleosome recognition by the chromosomal passenger complex

The paper determines the structure of the chromosomal passenger complex targeting module bound to phosphorylated nucleosomes, revealing a pivot-tether mechanism for Aurora B localization.

Nucleolin acute degradation reveals novel functions in cell cycle progression and division in TNBC

This study uses the AID system with CRISPR/Cas9 to acutely degrade nucleolin in TNBC cells, revealing its role in cell cycle progression and division, and showing that its abrogation enhances the efficacy of mitotic inhibitors.

Coarse-Grained Dynamic Simulation of Cytoskeletal Microtubule Twist

This study develops a coarse-grained model to simulate microtubule dynamics, validates it against experimental data, and demonstrates its effectiveness in simulating microtubule twist driven by motor proteins.

The mitotic chromosome periphery: a fluid coat that mediates chromosome mechanics

This study characterizes mitotic chromosome mechanics and shows the mitotic chromosome periphery (MCP) provides force-damping properties and structural constraints.

Microtubule association induces a Mg-free apo-like ADP pre-release conformation in kinesin-1 that is unaffected by its autoinhibitory tail

This study uses cryo-EM to reveal how microtubule association induces an Mg-free apo-like ADP pre-release conformation in kinesin-1, independent of tail interactions.

On the Curvature and Relaxation of Microtubule Plus-endTips

This study uses multiscale modeling to show that microtubule plus-end tips bend outward into curved structures regardless of GTP hydrolysis state, challenging existing models linking curvature to nucleotide state.

The intracellular bacterium Orientia tsutsugamushi hijacks the adaptor protein BICD2 for dynein-based motility

This study identifies how Orientia tsutsugamushi uses ScaC to recruit BICD2 for dynein-based motility, revealing a mechanism for bacterial transport within host cells.

Centromeres in the thermotolerant yeast K. marxianusmediate attachment to a single microtubule

This study shows that K. marxianus has a single centromeric nucleosome that mediates attachment to one microtubule, similar to S. cerevisiae.

Centrosomes act as organizing centers to promote Polo kinase-mediated adaptation to persistent DNA damage

The paper shows centrosomes organize Polo kinase (Cdc5) to adapt to DNA damage by phosphorylating SPB components, bypassing cell cycle arrest.

Anapc5 and Anapc7 as genetic modifiers of KIF18A function in fertility and mitotic progression

This study identifies Anapc5 and Anapc7 as genetic modifiers of KIF18A function in fertility and mitotic progression by analyzing mouse strain differences and cell line models.

PRC1 resists microtubule sliding in two distinct resistive modes due to variations in the separation between overlapping microtubules

The paper develops a computational model of PRC1 crosslinked microtubule pairs to show that braking and coasting modes depend on lateral separation, with braking reducing sliding speed more effectively.

Cell enlargement causes mitotic errors and aneuploidy in cells that evade senescence after CDK4/6 inhibition

The paper shows that CDK4/6 inhibitor-treated cells that evade senescence experience mitotic errors and aneuploidy due to cell enlargement, which can be rescued by restoring cohesion.

Structural basis for the interaction between the bacterial cell division proteins FtsZ and ZapA

This study reports the cryo-EM structure of the ZapA-FtsZ complex, revealing how ZapA interacts with FtsZ to regulate bacterial cell division.

Conformational flexibility of tubulin dimers regulates the transitions of microtubule dynamic instability

This study compares the conformational flexibility of Drosophila and bovine tubulins and links it to differences in microtubule dynamic instability.

14-3-3 epsilon inhibits premature centriole disengagement by inhibiting the activity of Plk1 and Separase.

This study shows that 14-3-3 epsilon inhibits centriole disengagement by suppressing Plk1 and Separase activity, leading to reduced cell proliferation and cell death.

Spectraplakin is required for dendritic microtubule organization via a tip-based mechanism

This study identifies spectraplakin Shot as essential for dendritic microtubule orientation via transient stabilization and tip-based nucleation during Drosophila development.

Spatiotemporal Orchestration of Mitosis by Cyclin-Dependent Kinase

This study shows CDK activation starts in the nucleus, not the cytoplasm, and reveals spatial differences in bistable responses during mitotic onset in fission yeast.

Mechanisms of microtubule dynamics from single-molecule measurements

This study identifies a new tubulin binding state on microtubule ends using single-molecule measurements and shows nucleotide effects on stability are allosteric.

Membrane-bound cargo carried by teams of motors with heterogeneous velocities go faster and further than rigid cargo

The paper uses simulations to show that motor velocity heterogeneity increases lipid cargo transport speed and distance compared to rigid cargo.

The BUB1 and BUBR1 paralogs scaffold the kinetochore corona

This study identifies two non-redundant pathways for kinetochore corona assembly involving BUB1 and BUBR1, revealing their roles in chromosome biorientation and checkpoint signaling.