Microtubule and mitosis dynamics

Branching, crosslinking and decentralization of microtubules accelerates intracellular assembly

This study uses a computational model to show that decentralized microtubule networks with branching and crosslinking optimize intracellular assembly processes by accelerating vesicle aggregation and spindle formation.

Dynamic remodeling of centrioles and the microtubule cytoskeleton in the lifecycle of chytrid fungi

The paper studies centriole and microtubule cytoskeleton remodeling in chytrid fungi during their lifecycle using bioinformatics, RNA sequencing, and expansion microscopy.

Multi-nucleation in two-cell human embryos stems from spindle and metaphase plate incoherence in the first mitosis

The paper investigates the causes of multi-nucleation in two-cell human embryos and finds that spindle and metaphase plate incoherence during the first mitosis leads to this phenomenon.

Boundary-Sensing Mechanism in Branched Microtubule Networks

This paper demonstrates a boundary-sensing mechanism in branched microtubule networks using a nanotechnology platform, showing how length-dependent branching and dynamic instability enable tunable network formation in confined spaces.

Kif11-haploinsufficient oocytes reveal spatially differential requirements for chromosome biorientation in the spindle

This study shows that Kif11 haploinsufficiency in mouse oocytes leads to impaired spindle bipolarization and spatially differential chromosome biorientation.

Expanding the Tubulin Code: TTLL11 Polyglutamylase Drives Elongation of Primary Tubulin Chains

This study identifies TTLL11 as a polyglutamylase that extends primary tubulin chains, revealing a new mechanism in the tubulin code and its interaction with other tubulin modifications.

Microtubule-dependent cell polarity regulates skin-resident macrophage phagocytosis and directed cell migration

This study shows that microtubules regulate Langerhans cell phagocytosis and directed migration in zebrafish skin by controlling dendrite morphology and navigation.

Monopolar orientation of kinetochores at meiosis is enforced by COHESINS and their regulators, CENP-C, and the deSUMOylase SPF2

This study identifies molecular components enforcing monopolar kinetochore orientation in meiosis I of Arabidopsis thaliana, showing cohesin, CENP-C, and SPF2 are essential.

Coin-tossing by Kinesin-1 Head and Tail Binding Collectively Drives Microtubule Patterns

The paper shows that kinesin-1's head and tail domains collectively drive microtubule motion patterns through a coin-toss mechanism, with the tail acting as an active mechanical component.

CENcyclopedia: Dynamic Landscape of Kinetochore Architecture Throughout the Cell Cycle

This study maps kinetochore protein dynamics across the cell cycle using quantitative immunofluorescence, revealing how composition changes to support chromosome segregation.

A new nanoDSF approach to anti-tubulin compounds screening revealed novel MTAs among approved drugs.

The paper introduces a nanoDSF method to screen for anti-tubulin compounds, identifying novel MTAs among approved drugs and demonstrating its effectiveness in detecting known and new agents.

Force Propagation in Active Cytoskeletal Networks

This paper shows that increasing microtubule bundle length in active networks triggers a percolation transition, enabling global force generation and mechanical work.

Structural insights into SSNA1 self-assembly and its microtubule binding for centriole maintenance

This study determines the cryo-EM structure of SSNA-1 and shows its self-assembly and microtubule-binding role in centriole maintenance and embryonic viability.

Polo-like kinase 1 maintains transcription and chromosomal accessibility during mitosis

This study identifies Polo-like kinase 1 (Plk1) as a regulator of mitotic transcription, showing its role in maintaining chromosomal accessibility and transcriptional activity during mitosis.

Mechanisms of nuclear segregation in a multinucleate multibudding yeast

This study examines mitotic nuclear segregation in the multibudding yeast Aureobasidium pullulans, revealing distinct mechanisms compared to uninucleate yeasts.

Crosstalk between tubulin glutamylation and tyrosination regulates kinesin-3-mediated axonal transport

This study shows that tubulin glutamylation and tyrosination crosstalk regulates kinesin-3 motor function and axonal transport in C. elegans neurons.

A fluid droplet harvests the force generated by shrinking microtubules in living cells

The paper shows that biomolecular condensates in budding yeast capture forces from shrinking microtubules to move the mitotic spindle.

Basal autophagy during meiotic prophase I is required for accurate chromosome segregation in Drosophila oocytes and declines during oocyte aging

This study shows that autophagy decline in aging Drosophila oocytes leads to cohesion loss and segregation errors during meiosis.

Functional genetics with a hypomorphic CENP-C mutant reveals a regulatory system for chromosome congression

The paper identifies KIF18A as a gene whose knockout causes synthetic lethality with a hypomorphic CENP-C mutant, revealing a regulatory system for chromosome congression.

BRD4 regulates Aurora B kinase activity

This study shows BRD4 inhibits Aurora B kinase activity during mitosis by binding to it, and JNK phosphorylates BRD4 to release it, allowing Aurora B activation.

NuMA mechanically reinforces the spindle independently of its partner dynein

This study shows NuMA reinforces the spindle mechanically through microtubule crosslinking, independent of dynein, by bundling microtubules and stabilizing spindle poles under force.

Transcriptional repression of central spindle factors controls endomitosis in the C. elegans intestine

This study identifies transcriptional repression of cytokinesis regulators as a mechanism controlling endomitosis in C. elegans intestinal cells.

A novel mechanism for centrosome expulsion ensures metabolic activity in polyploid cells

The paper identifies a novel centrosome elimination mechanism in Drosophila polyploid cells that supports metabolic activity by preserving mitochondrial function.

Discovery of unique mitotic mechanisms in Paradiplonema papillatum

This study identifies unique mitotic features in Paradiplonema papillatum using fluorescence microscopy and YFP-tagging, revealing novel structures like metaphase rings and potential kinetochore localization.

Cryo-EM captures early intermediate steps in dynein activation by LIS1

The paper uses cryo-EM to identify an early intermediate state in dynein activation by LIS1, termed Pre-Chi, and shows its functional importance through mutational analysis.

Nde1 Promotes Lis1 Binding to Full-Length Autoinhibited Human Dynein-1

The paper shows Nde1 enhances Lis1 binding to autoinhibited dynein, facilitating Phi conformation opening through a novel intermediate state.

Brain biomechanics governs mitotic fidelity of embryonic neural progenitors

This study shows that biomechanical stress in embryonic neural progenitors affects mitotic fidelity through cortical actin and tension.

Synaptic vesicle glycoprotein 2 enables viable aneuploidy following centrosome amplification

The paper identifies a gene that promotes viability in cells with centrosome amplification and multipolar division by positioning centrosomes at the plasma membrane.

Beta-hairpin Mechanism of Autoinhibition and Activation in the Kinesin-2 Family

The paper identifies a beta-hairpin motif in kinesin-2 that regulates motility by sequestering motor domains, revealing its role in activation and coordination with dynein.

Origin of Chromosome 12 Trisomy Surge in Human Induced Pluripotent Stem Cells (iPSCs)

This paper challenges the conventional view of trisomy 12 origin in iPSCs by showing it arises de novo in diploid cells through anaphase bridging and micronuclei formation, leading to rapid dominance of trisomic cells.